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- W4363646890 abstract "Abstract Kinesin‐1, a motor protein composed of the kinesin heavy chain (KHC) and the kinesin light chain (KLC), is essential for proper cellular morphogenesis and function. A monoclonal antibody (mAb) called H2 recognizes the KHC in a broad range of species and is one of the most widely used mAbs in cytoskeletal motor research. Here, we present vectors that express recombinant H2 in mammalian cells. We show the recombinant H2 performs as well as the hybridoma‐derived H2 in both western blotting and immunofluorescence assays. Additionally, the recombinant H2 can detect all three human KHC isotypes (KIF5A, KIF5B, and KIF5C) and amyotrophic lateral sclerosis‐associated KIF5A aggregates in cells. In addition, we developed a chickenized version of the H2 mAb's single chain variable fragment, which can be used in immunofluorescence microscopy and expands the potential applications of H2. Overall, our results demonstrate that recombinant H2 is a useful tool for studying the functions of KHCs." @default.
- W4363646890 created "2023-04-11" @default.
- W4363646890 creator A5052207778 @default.
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- W4363646890 date "2023-04-10" @default.
- W4363646890 modified "2023-10-15" @default.
- W4363646890 title "Generation of recombinant and chickenized <scp>scFv</scp> versions of an anti‐kinesin monoclonal antibody <scp>H2</scp>" @default.
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- W4363646890 doi "https://doi.org/10.1002/cm.21756" @default.
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- W4363646890 hasPublicationYear "2023" @default.
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