FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4363651567> ?p ?o ?g. }

• W4363651567 abstract "Immune cells and immune microenvironment play important in the evolution of sepsis. This study aimed to explore hub genes related to the abundance of immune cell infiltration in sepsis. The GEOquery package is used to download and organize data from the GEO database. A total of 61 differentially expressed genes (DEGs) between sepsis samples and normal samples were obtained through the ‘limma’ package. T cells, natural killer (NK) cells, monocytes, megakaryocytes, dendritic cells (DCs), and B cells formed six distinct clusters on the t-distributed stochastic neighbor embedding (t-SNE) plot generated using the Seurat R package. Gene set enrichment analysis (GSEA) enrichment analysis showed that sepsis samples and normal samples were related to Neutrophil Degranulation, Modulators of Tcr Signaling and T Cell Activation, IL 17 Pathway, T Cell Receptor Signaling Pathway, Ctl Pathway, Immunoregulatory Interactions Between a Lymphoid and A Non-Lymphoid Cell. GO analysis and KEGG analysis of immune-related genes showed that the intersection genes were mainly associated with Immune-related signaling pathways. Seven hub genes (CD28, CD3D, CD2, CD4, IL7R, LCK, and CD3E) were screened using Maximal Clique Centrality, Maximum neighborhood component, and Density of Maximum Neighborhood Component algorithms. The lower expression of the six hub genes (CD28, CD3D, CD4, IL7R, LCK, and CD3E) was observed in sepsis samples. We observed the significant difference of several immune cell between sepsis samples and control samples. Finally, we carried out in vivo animal experiments, including Western blotting, flow cytometry, Elisa, and qPCR assays to detect the concentration and the expression of several immune factors." @default.
• W4363651567 created "2023-04-11" @default.
• W4363651567 creator A5000326646 @default.
• W4363651567 creator A5002592823 @default.
• W4363651567 creator A5003288592 @default.
• W4363651567 creator A5032577218 @default.
• W4363651567 creator A5072960229 @default.
• W4363651567 date "2023-04-11" @default.
• W4363651567 modified "2023-09-30" @default.
• W4363651567 title "Single-cell transcriptomics reveals immune infiltrate in sepsis" @default.
• W4363651567 cites W1984883254 @default.
• W4363651567 cites W2061531106 @default.
• W4363651567 cites W2083513831 @default.
• W4363651567 cites W2085316561 @default.
• W4363651567 cites W2093260830 @default.
• W4363651567 cites W2100239923 @default.
• W4363651567 cites W2112798365 @default.
• W4363651567 cites W2114704109 @default.
• W4363651567 cites W2116634113 @default.
• W4363651567 cites W2146512944 @default.
• W4363651567 cites W2159707944 @default.
• W4363651567 cites W2330589594 @default.
• W4363651567 cites W2332078202 @default.
• W4363651567 cites W2386572545 @default.
• W4363651567 cites W2737162863 @default.
• W4363651567 cites W2790790020 @default.
• W4363651567 cites W2794480084 @default.
• W4363651567 cites W2905194441 @default.
• W4363651567 cites W2905983446 @default.
• W4363651567 cites W2907514116 @default.
• W4363651567 cites W2915104822 @default.
• W4363651567 cites W2949177718 @default.
• W4363651567 cites W3004780794 @default.
• W4363651567 cites W3006033724 @default.
• W4363651567 cites W3007094285 @default.
• W4363651567 cites W3034168389 @default.
• W4363651567 cites W3041978440 @default.
• W4363651567 cites W3047284551 @default.
• W4363651567 cites W3048884493 @default.
• W4363651567 cites W3081449785 @default.
• W4363651567 cites W3088028359 @default.
• W4363651567 cites W3091447756 @default.
• W4363651567 cites W3102381532 @default.
• W4363651567 cites W3103081155 @default.
• W4363651567 cites W3128961456 @default.
• W4363651567 cites W3133926930 @default.
• W4363651567 cites W3164692211 @default.
• W4363651567 cites W3180328126 @default.
• W4363651567 cites W3210860076 @default.
• W4363651567 cites W3214720083 @default.
• W4363651567 cites W4206833715 @default.
• W4363651567 cites W638613648 @default.
• W4363651567 doi "https://doi.org/10.3389/fphar.2023.1133145" @default.
• W4363651567 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37113759" @default.
• W4363651567 hasPublicationYear "2023" @default.
• W4363651567 type Work @default.
• W4363651567 citedByCount "0" @default.
• W4363651567 crossrefType "journal-article" @default.
• W4363651567 hasAuthorship W4363651567A5000326646 @default.
• W4363651567 hasAuthorship W4363651567A5002592823 @default.
• W4363651567 hasAuthorship W4363651567A5003288592 @default.
• W4363651567 hasAuthorship W4363651567A5032577218 @default.
• W4363651567 hasAuthorship W4363651567A5072960229 @default.
• W4363651567 hasBestOaLocation W43636515671 @default.
• W4363651567 hasConcept C104317684 @default.
• W4363651567 hasConcept C123982805 @default.
• W4363651567 hasConcept C148125776 @default.
• W4363651567 hasConcept C150194340 @default.
• W4363651567 hasConcept C162317418 @default.
• W4363651567 hasConcept C19317047 @default.
• W4363651567 hasConcept C203014093 @default.
• W4363651567 hasConcept C2776090121 @default.
• W4363651567 hasConcept C54355233 @default.
• W4363651567 hasConcept C553184892 @default.
• W4363651567 hasConcept C79484868 @default.
• W4363651567 hasConcept C86803240 @default.
• W4363651567 hasConcept C8891405 @default.
• W4363651567 hasConcept C95444343 @default.
• W4363651567 hasConceptScore W4363651567C104317684 @default.
• W4363651567 hasConceptScore W4363651567C123982805 @default.
• W4363651567 hasConceptScore W4363651567C148125776 @default.
• W4363651567 hasConceptScore W4363651567C150194340 @default.
• W4363651567 hasConceptScore W4363651567C162317418 @default.
• W4363651567 hasConceptScore W4363651567C19317047 @default.
• W4363651567 hasConceptScore W4363651567C203014093 @default.
• W4363651567 hasConceptScore W4363651567C2776090121 @default.
• W4363651567 hasConceptScore W4363651567C54355233 @default.
• W4363651567 hasConceptScore W4363651567C553184892 @default.
• W4363651567 hasConceptScore W4363651567C79484868 @default.
• W4363651567 hasConceptScore W4363651567C86803240 @default.
• W4363651567 hasConceptScore W4363651567C8891405 @default.
• W4363651567 hasConceptScore W4363651567C95444343 @default.
• W4363651567 hasLocation W43636515671 @default.
• W4363651567 hasLocation W43636515672 @default.
• W4363651567 hasLocation W43636515673 @default.
• W4363651567 hasOpenAccess W4363651567 @default.
• W4363651567 hasPrimaryLocation W43636515671 @default.
• W4363651567 hasRelatedWork W1542374316 @default.
• W4363651567 hasRelatedWork W1995507496 @default.
• W4363651567 hasRelatedWork W2037024992 @default.

• next