FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4363672359> ?p ?o ?g. }

• W4363672359 abstract "Introduction Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a commonly occurring sequelae of traumatic injury resulting from indirect insults like hypovolemic shock and/or extrapulmonary sepsis. The high lethality rate associated with these pathologies outlines the importance of clarifying the “priming” effects seen in the post-shock lung microenvironment, which are understood to bring about a dysregulated or overt immune response when triggered by a secondary systemic infectious/septic challenge culminating in ALI. In this pilot project, we test the hypothesis that application of a single cell multiomics approach can elucidate novel phenotype specific pathways potentially contributing to shock-induced ALI/ARDS. Methods Hypovolemic shock was induced in C57BL/6 (wild-type), PD-1, PD-L1, or VISTA gene deficient male mice, 8–12 weeks old. Wild-type sham surgeries function as negative controls. A total of 24-h post-shock rodents were sacrificed, their lungs harvested and sectioned, with pools prepared from 2 mice per background, and flash frozen on liquid nitrogen. N = 2 biological replicates (representing 4 mice total) were achieved for all treatment groups across genetic backgrounds. Samples were received by the Boas Center for Genomics and Human Genetics, where single cell multiomics libraries were prepared for RNA/ATAC sequencing. The analysis pipeline Cell Ranger ARC was implemented to attain feature linkage assessments across genes of interest. Results Sham (pre-shock) results suggest high chromatin accessibility around calcitonin receptor like receptor (CALCRL) across cellular phenotypes with 17 and 18 feature links, exhibiting positive correlation with gene expression between biological replicates. Similarity between both sample chromatin profiles/linkage arcs is evident. Post-shock wild-type accessibility is starkly reduced across replicates where the number of feature links drops to 1 and 3, again presenting similar replicate profiles. Samples from shocked gene deficient backgrounds displayed high accessibility and similar profiles to the pre-shock lung microenvironment. Conclusion High pre-shock availability of DNA segments and their positive correlation with CALCRL gene expression suggests an apparent regulatory capacity on transcription. Post-shock gene deficient chromatin profiles presented similar results to that of pre-shock wild-type samples, suggesting an influence on CALCRL accessibility. Key changes illustrated in the pre-ALI context of shock may allow for additional resolution of “priming” and “cellular pre-activation/pre-disposition” processes within the lung microenvironment." @default.
• W4363672359 created "2023-04-11" @default.
• W4363672359 creator A5003446329 @default.
• W4363672359 creator A5041323832 @default.
• W4363672359 creator A5062853597 @default.
• W4363672359 creator A5065918774 @default.
• W4363672359 creator A5072286032 @default.
• W4363672359 creator A5074441316 @default.
• W4363672359 creator A5085134753 @default.
• W4363672359 creator A5087261089 @default.
• W4363672359 date "2023-04-11" @default.
• W4363672359 modified "2023-10-14" @default.
• W4363672359 title "Application of single cell multiomics points to changes in chromatin accessibility near calcitonin receptor like receptor and a possible role for adrenomedullin in the post-shock lung" @default.
• W4363672359 cites W1531821806 @default.
• W4363672359 cites W1572739876 @default.
• W4363672359 cites W1600560134 @default.
• W4363672359 cites W1831064357 @default.
• W4363672359 cites W1976081136 @default.
• W4363672359 cites W1984545852 @default.
• W4363672359 cites W1994174891 @default.
• W4363672359 cites W1994176403 @default.
• W4363672359 cites W2007408888 @default.
• W4363672359 cites W2013443224 @default.
• W4363672359 cites W2041194473 @default.
• W4363672359 cites W2050094423 @default.
• W4363672359 cites W2056893613 @default.
• W4363672359 cites W2061730726 @default.
• W4363672359 cites W2084986980 @default.
• W4363672359 cites W2096098099 @default.
• W4363672359 cites W2106848118 @default.
• W4363672359 cites W2107556275 @default.
• W4363672359 cites W2109108939 @default.
• W4363672359 cites W2111143066 @default.
• W4363672359 cites W2114769524 @default.
• W4363672359 cites W2140668032 @default.
• W4363672359 cites W2162641630 @default.
• W4363672359 cites W2166932124 @default.
• W4363672359 cites W2243947562 @default.
• W4363672359 cites W2280404143 @default.
• W4363672359 cites W2297960835 @default.
• W4363672359 cites W2512365985 @default.
• W4363672359 cites W2560827811 @default.
• W4363672359 cites W2606939149 @default.
• W4363672359 cites W2620241325 @default.
• W4363672359 cites W2791367003 @default.
• W4363672359 cites W2905875983 @default.
• W4363672359 cites W2912390237 @default.
• W4363672359 cites W2938910572 @default.
• W4363672359 cites W2953064750 @default.
• W4363672359 cites W2981600638 @default.
• W4363672359 cites W3006692724 @default.
• W4363672359 cites W3020992950 @default.
• W4363672359 cites W3034102724 @default.
• W4363672359 cites W3037339341 @default.
• W4363672359 cites W3080945506 @default.
• W4363672359 cites W3087169700 @default.
• W4363672359 cites W3122035292 @default.
• W4363672359 cites W3151709979 @default.
• W4363672359 cites W3156113107 @default.
• W4363672359 cites W3214280461 @default.
• W4363672359 cites W4206067467 @default.
• W4363672359 cites W4210368582 @default.
• W4363672359 cites W4211250103 @default.
• W4363672359 cites W4220711471 @default.
• W4363672359 cites W4228999954 @default.
• W4363672359 doi "https://doi.org/10.3389/fmed.2023.1003121" @default.
• W4363672359 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37113606" @default.
• W4363672359 hasPublicationYear "2023" @default.
• W4363672359 type Work @default.
• W4363672359 citedByCount "0" @default.
• W4363672359 crossrefType "journal-article" @default.
• W4363672359 hasAuthorship W4363672359A5003446329 @default.
• W4363672359 hasAuthorship W4363672359A5041323832 @default.
• W4363672359 hasAuthorship W4363672359A5062853597 @default.
• W4363672359 hasAuthorship W4363672359A5065918774 @default.
• W4363672359 hasAuthorship W4363672359A5072286032 @default.
• W4363672359 hasAuthorship W4363672359A5074441316 @default.
• W4363672359 hasAuthorship W4363672359A5085134753 @default.
• W4363672359 hasAuthorship W4363672359A5087261089 @default.
• W4363672359 hasBestOaLocation W43636723591 @default.
• W4363672359 hasConcept C104317684 @default.
• W4363672359 hasConcept C126322002 @default.
• W4363672359 hasConcept C127716648 @default.
• W4363672359 hasConcept C142724271 @default.
• W4363672359 hasConcept C203014093 @default.
• W4363672359 hasConcept C2776348555 @default.
• W4363672359 hasConcept C2777714996 @default.
• W4363672359 hasConcept C54355233 @default.
• W4363672359 hasConcept C71924100 @default.
• W4363672359 hasConcept C86803240 @default.
• W4363672359 hasConceptScore W4363672359C104317684 @default.
• W4363672359 hasConceptScore W4363672359C126322002 @default.
• W4363672359 hasConceptScore W4363672359C127716648 @default.
• W4363672359 hasConceptScore W4363672359C142724271 @default.
• W4363672359 hasConceptScore W4363672359C203014093 @default.
• W4363672359 hasConceptScore W4363672359C2776348555 @default.
• W4363672359 hasConceptScore W4363672359C2777714996 @default.
• W4363672359 hasConceptScore W4363672359C54355233 @default.
• W4363672359 hasConceptScore W4363672359C71924100 @default.
• W4363672359 hasConceptScore W4363672359C86803240 @default.

• next