Matches in SemOpenAlex for { <https://semopenalex.org/work/W4363673732> ?p ?o ?g. }
- W4363673732 abstract "How are ions distributed in the three-dimensional (3D) volume confined in a nanoscale compartment? Regulation of ionic flow in the intracellular milieu has been explained by different theoretical models and experimentally demonstrated for several compartments with microscale dimensions. Most of these models predict a homogeneous distribution of ions seconds or milliseconds after an initial diffusion step formed at the ion translocation site, leaving open questions when it comes to ion/element distribution in spaces/compartments with nanoscale dimensions. Due to the influence of compartment size on the regulation of ionic flow, theoretical variations of classical models have been proposed, suggesting heterogeneous distributions of ions/elements within nanoscale compartments. Nonetheless, such assumptions have not been fully proven for the 3D volume of an organelle. In this work, we used a combination of cutting-edge electron microscopy techniques to map the 3D distribution of diffusible elements within the whole volume of acidocalcisomes in trypanosomes. Cryofixed cells were analyzed by scanning transmission electron microscopy tomography combined with elemental mapping using a high-performance setup of X-ray detectors. Results showed the existence of elemental nanodomains within the acidocalcisomes, where cationic elements display a self-excluding pattern. These were validated by Pearson correlation analysis and in silico molecular dynamic simulations. Formation of element domains within the 3D space of an organelle is demonstrated. Distribution patterns that support the electrodiffusion theory proposed for nanophysiology models have been found. The experimental pipeline shown here can be applied to a variety of models where ion mobilization plays a crucial role in physiological processes." @default.
- W4363673732 created "2023-04-11" @default.
- W4363673732 creator A5014123016 @default.
- W4363673732 creator A5035663498 @default.
- W4363673732 creator A5036531858 @default.
- W4363673732 creator A5041985978 @default.
- W4363673732 creator A5072006546 @default.
- W4363673732 creator A5078076153 @default.
- W4363673732 date "2023-04-10" @default.
- W4363673732 modified "2023-10-12" @default.
- W4363673732 title "A spatially resolved elemental nanodomain organization within acidocalcisomes in <i>Trypanosoma cruzi</i>" @default.
- W4363673732 cites W1567438378 @default.
- W4363673732 cites W1575119316 @default.
- W4363673732 cites W1579542494 @default.
- W4363673732 cites W1933778251 @default.
- W4363673732 cites W1964549513 @default.
- W4363673732 cites W1966521314 @default.
- W4363673732 cites W1971943159 @default.
- W4363673732 cites W1979348931 @default.
- W4363673732 cites W1994941561 @default.
- W4363673732 cites W1997266228 @default.
- W4363673732 cites W1998722531 @default.
- W4363673732 cites W2001923641 @default.
- W4363673732 cites W2002315028 @default.
- W4363673732 cites W2005647067 @default.
- W4363673732 cites W2008002968 @default.
- W4363673732 cites W2010557882 @default.
- W4363673732 cites W2013295580 @default.
- W4363673732 cites W2015688756 @default.
- W4363673732 cites W2024374022 @default.
- W4363673732 cites W2028046413 @default.
- W4363673732 cites W2029101220 @default.
- W4363673732 cites W2031696596 @default.
- W4363673732 cites W2035266068 @default.
- W4363673732 cites W2035687084 @default.
- W4363673732 cites W2039560970 @default.
- W4363673732 cites W2040810713 @default.
- W4363673732 cites W2045683699 @default.
- W4363673732 cites W2051168595 @default.
- W4363673732 cites W2052042258 @default.
- W4363673732 cites W2053099864 @default.
- W4363673732 cites W2053986750 @default.
- W4363673732 cites W2061206540 @default.
- W4363673732 cites W2061496678 @default.
- W4363673732 cites W2067223665 @default.
- W4363673732 cites W2069177804 @default.
- W4363673732 cites W2070330324 @default.
- W4363673732 cites W2075400125 @default.
- W4363673732 cites W2083164919 @default.
- W4363673732 cites W2083884362 @default.
- W4363673732 cites W2092377076 @default.
- W4363673732 cites W2092390379 @default.
- W4363673732 cites W2094785232 @default.
- W4363673732 cites W2110421505 @default.
- W4363673732 cites W2113351822 @default.
- W4363673732 cites W2118233996 @default.
- W4363673732 cites W2121929754 @default.
- W4363673732 cites W2124801955 @default.
- W4363673732 cites W2128124394 @default.
- W4363673732 cites W2133815054 @default.
- W4363673732 cites W2139054237 @default.
- W4363673732 cites W2139066576 @default.
- W4363673732 cites W2157854661 @default.
- W4363673732 cites W2167279371 @default.
- W4363673732 cites W2169872783 @default.
- W4363673732 cites W2171426976 @default.
- W4363673732 cites W2285389784 @default.
- W4363673732 cites W2336127465 @default.
- W4363673732 cites W2336589732 @default.
- W4363673732 cites W2345219027 @default.
- W4363673732 cites W2404924867 @default.
- W4363673732 cites W2499278905 @default.
- W4363673732 cites W2508709614 @default.
- W4363673732 cites W2564539626 @default.
- W4363673732 cites W2590009627 @default.
- W4363673732 cites W2590568317 @default.
- W4363673732 cites W2614044637 @default.
- W4363673732 cites W2766886748 @default.
- W4363673732 cites W2767435910 @default.
- W4363673732 cites W2778338649 @default.
- W4363673732 cites W2785867881 @default.
- W4363673732 cites W2889451506 @default.
- W4363673732 cites W2889738437 @default.
- W4363673732 cites W2944266770 @default.
- W4363673732 cites W2997916175 @default.
- W4363673732 cites W3038124667 @default.
- W4363673732 cites W3047259174 @default.
- W4363673732 cites W3082191187 @default.
- W4363673732 cites W3111148689 @default.
- W4363673732 cites W3119897429 @default.
- W4363673732 cites W3151649211 @default.
- W4363673732 cites W3166654832 @default.
- W4363673732 cites W4205363778 @default.
- W4363673732 cites W4205935230 @default.
- W4363673732 cites W4206924621 @default.
- W4363673732 cites W4210992500 @default.
- W4363673732 cites W4212851483 @default.
- W4363673732 cites W4225684865 @default.
- W4363673732 cites W4229011489 @default.
- W4363673732 cites W4285188369 @default.