FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4364354601> ?p ?o ?g. }

• W4364354601 abstract "Horseshoe crab (Tachypleus gigas) amebocytes are useful biomedical components for endotoxin detection, and their growing needs for biomedical purposes cause the horseshoe crab population to decline. Factor C synthesis via genetic engineering offers a solution to replace natural horseshoe crab's factor C and prevent its excessive harvest from nature. In response to these concerns, this study aimed to characterize the amebocyte lysates and factor C protein modeling of T. gigas originated from Banyuasin South Sumatra Estuary.Sampling of T. gigas was carried out in Banyuasin South Sumatra Estuary, Indonesia. The endotoxin test or TAL (Tachypleus amebocyte lysates) assay was performed using gel coagulation method. Protein characterization of protease enzyme was conducted by protease activity, SDS-PAGE, and zymogram analysis. The cDNA of mitochondrial COI gene was amplified for molecular identification followed by cDNA cloning of factor C. Protein modeling was investigated by molecular docking and molecular dynamic (MD) simulation. Endotoxin test results showed that TAL-35 had endotoxin sensitivity in a range of 0.0156-1 EU/ml, while TAL 36 had a sensitivity between 00,625 and 1 EU/ml. T. gigas amebocytes have protease activity in molecular mass sizes less than 60 kDa, with 367 U/ml for TAL 35 and 430 U/ml for TAL 36. The molecular identification revealed 98.68% identity similarity to T. gigas. The docking results suggested three ligands; i.e., diphosphoryl lipid A, core lipid A, and Kdo2 lipid A can be activators of the factor C protein by binding to the region of the receptor to form a ligand-receptor complex.Endotoxins can be detected using horseshoe crab amebocytes. The presence of proteases is considered responsible for this ability, as evidenced by casein zymogram results. According to docking and MD analysis, we found that lipopolysaccharides (LPS) participate to the binding site of factor C." @default.
• W4364354601 created "2023-04-12" @default.
• W4364354601 creator A5013611545 @default.
• W4364354601 creator A5014220387 @default.
• W4364354601 creator A5015615514 @default.
• W4364354601 creator A5017615616 @default.
• W4364354601 creator A5029563273 @default.
• W4364354601 creator A5032465585 @default.
• W4364354601 creator A5036811971 @default.
• W4364354601 creator A5041796227 @default.
• W4364354601 creator A5041967595 @default.
• W4364354601 creator A5052816710 @default.
• W4364354601 creator A5053765301 @default.
• W4364354601 date "2023-04-11" @default.
• W4364354601 modified "2023-09-25" @default.
• W4364354601 title "Characterization, protein modeling, and molecular docking of factor C from Indonesian horseshoe crab (Tachypleus gigas)" @default.
• W4364354601 cites W1572480128 @default.
• W4364354601 cites W1603231983 @default.
• W4364354601 cites W1848636460 @default.
• W4364354601 cites W1975374563 @default.
• W4364354601 cites W197812759 @default.
• W4364354601 cites W2006873867 @default.
• W4364354601 cites W2018175604 @default.
• W4364354601 cites W2044031697 @default.
• W4364354601 cites W2044853461 @default.
• W4364354601 cites W2048233935 @default.
• W4364354601 cites W2054411897 @default.
• W4364354601 cites W2065971267 @default.
• W4364354601 cites W2083617813 @default.
• W4364354601 cites W2104097901 @default.
• W4364354601 cites W2115167726 @default.
• W4364354601 cites W2147443001 @default.
• W4364354601 cites W2202719728 @default.
• W4364354601 cites W2217491380 @default.
• W4364354601 cites W2324791959 @default.
• W4364354601 cites W2369813939 @default.
• W4364354601 cites W2480602172 @default.
• W4364354601 cites W2504621363 @default.
• W4364354601 cites W2561546745 @default.
• W4364354601 cites W2620727311 @default.
• W4364354601 cites W2624970601 @default.
• W4364354601 cites W2801223332 @default.
• W4364354601 cites W2801906762 @default.
• W4364354601 cites W2895703485 @default.
• W4364354601 cites W2964623973 @default.
• W4364354601 cites W3012224671 @default.
• W4364354601 cites W3013572772 @default.
• W4364354601 cites W3014483120 @default.
• W4364354601 cites W3015438624 @default.
• W4364354601 cites W3081464678 @default.
• W4364354601 cites W3177338305 @default.
• W4364354601 cites W3194726633 @default.
• W4364354601 cites W3199742783 @default.
• W4364354601 cites W4281790889 @default.
• W4364354601 cites W4295038067 @default.
• W4364354601 cites W4295165394 @default.
• W4364354601 cites W4308616064 @default.
• W4364354601 doi "https://doi.org/10.1186/s43141-023-00496-8" @default.
• W4364354601 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37040022" @default.
• W4364354601 hasPublicationYear "2023" @default.
• W4364354601 type Work @default.
• W4364354601 citedByCount "0" @default.
• W4364354601 crossrefType "journal-article" @default.
• W4364354601 hasAuthorship W4364354601A5013611545 @default.
• W4364354601 hasAuthorship W4364354601A5014220387 @default.
• W4364354601 hasAuthorship W4364354601A5015615514 @default.
• W4364354601 hasAuthorship W4364354601A5017615616 @default.
• W4364354601 hasAuthorship W4364354601A5029563273 @default.
• W4364354601 hasAuthorship W4364354601A5032465585 @default.
• W4364354601 hasAuthorship W4364354601A5036811971 @default.
• W4364354601 hasAuthorship W4364354601A5041796227 @default.
• W4364354601 hasAuthorship W4364354601A5041967595 @default.
• W4364354601 hasAuthorship W4364354601A5052816710 @default.
• W4364354601 hasAuthorship W4364354601A5053765301 @default.
• W4364354601 hasBestOaLocation W43643546011 @default.
• W4364354601 hasConcept C104317684 @default.
• W4364354601 hasConcept C153911025 @default.
• W4364354601 hasConcept C169627665 @default.
• W4364354601 hasConcept C181199279 @default.
• W4364354601 hasConcept C187882448 @default.
• W4364354601 hasConcept C18903297 @default.
• W4364354601 hasConcept C2776287654 @default.
• W4364354601 hasConcept C2776714187 @default.
• W4364354601 hasConcept C55493867 @default.
• W4364354601 hasConcept C86803240 @default.
• W4364354601 hasConceptScore W4364354601C104317684 @default.
• W4364354601 hasConceptScore W4364354601C153911025 @default.
• W4364354601 hasConceptScore W4364354601C169627665 @default.
• W4364354601 hasConceptScore W4364354601C181199279 @default.
• W4364354601 hasConceptScore W4364354601C187882448 @default.
• W4364354601 hasConceptScore W4364354601C18903297 @default.
• W4364354601 hasConceptScore W4364354601C2776287654 @default.
• W4364354601 hasConceptScore W4364354601C2776714187 @default.
• W4364354601 hasConceptScore W4364354601C55493867 @default.
• W4364354601 hasConceptScore W4364354601C86803240 @default.
• W4364354601 hasIssue "1" @default.
• W4364354601 hasLocation W43643546011 @default.
• W4364354601 hasLocation W43643546012 @default.
• W4364354601 hasLocation W43643546013 @default.
• W4364354601 hasOpenAccess W4364354601 @default.

• next