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- W4365455576 abstract "Nosema is a diverse genus of unicellular microsporidian parasites of insects and other arthropods. Nosema muscidifuracis infects parasitoid wasp species of Muscidifurax zaraptor and M. raptor (Hymenoptera: Pteromalidae), causing ~50% reduction in longevity and ~90% reduction in fecundity.Here, we report the first assembly of the N. muscidifuracis genome (14,397,169 bp in 28 contigs) of high continuity (contig N50 544.3 Kb) and completeness (BUSCO score 97.0%). A total of 2,782 protein-coding genes were annotated, with 66.2% of the genes having two copies and 24.0% of genes having three copies. These duplicated genes are highly similar, with a sequence identity of 99.3%. The complex pattern suggests extensive gene duplications and rearrangements across the genome. We annotated 57 rDNA loci, which are highly GC-rich (37%) in a GC-poor genome (25% genome average). Nosema-specific qPCR primer sets were designed based on 18S rDNA annotation as a diagnostic tool to determine its titer in host samples. We discovered high Nosema titers in Nosema-cured M. raptor and M. zaraptor using heat treatment in 2017 and 2019, suggesting that the remedy did not completely eliminate the Nosema infection. Cytogenetic analyses revealed heavy infections of N. muscidifuracis within the ovaries of M. raptor and M. zaraptor, consistent with the titer determined by qPCR and suggesting a heritable component of infection and per ovum vertical transmission.The parasitoids-Nosema system is laboratory tractable and, therefore, can serve as a model to inform future genome manipulations of Nosema-host system for investigations of Nosemosis." @default.
- W4365455576 created "2023-04-15" @default.
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- W4365455576 date "2023-04-13" @default.
- W4365455576 modified "2023-10-16" @default.
- W4365455576 title "New insights into the genome and transmission of the microsporidian pathogen Nosema muscidifuracis" @default.
- W4365455576 cites W1114690831 @default.
- W4365455576 cites W133678739 @default.
- W4365455576 cites W1554038937 @default.
- W4365455576 cites W1964510698 @default.
- W4365455576 cites W1966795732 @default.
- W4365455576 cites W1975800767 @default.
- W4365455576 cites W1982372752 @default.
- W4365455576 cites W1983039406 @default.
- W4365455576 cites W1996700726 @default.
- W4365455576 cites W2011657487 @default.
- W4365455576 cites W2013805140 @default.
- W4365455576 cites W2016366315 @default.
- W4365455576 cites W2020134788 @default.
- W4365455576 cites W2022802974 @default.
- W4365455576 cites W2024302465 @default.
- W4365455576 cites W2027518719 @default.
- W4365455576 cites W2037869053 @default.
- W4365455576 cites W2041782952 @default.
- W4365455576 cites W2043639460 @default.
- W4365455576 cites W2048590922 @default.
- W4365455576 cites W2052853820 @default.
- W4365455576 cites W2055833101 @default.
- W4365455576 cites W2062315170 @default.
- W4365455576 cites W2062865201 @default.
- W4365455576 cites W2067897418 @default.
- W4365455576 cites W2073795090 @default.
- W4365455576 cites W2074231493 @default.
- W4365455576 cites W2082560252 @default.
- W4365455576 cites W2084708244 @default.
- W4365455576 cites W2097050932 @default.
- W4365455576 cites W2097204912 @default.
- W4365455576 cites W2104121186 @default.
- W4365455576 cites W2108230379 @default.
- W4365455576 cites W2112814753 @default.
- W4365455576 cites W2115011276 @default.
- W4365455576 cites W2119169645 @default.
- W4365455576 cites W2119180969 @default.
- W4365455576 cites W2122137006 @default.
- W4365455576 cites W2126763121 @default.
- W4365455576 cites W2131271579 @default.
- W4365455576 cites W2138480342 @default.
- W4365455576 cites W2141152740 @default.
- W4365455576 cites W2141223573 @default.
- W4365455576 cites W2144268209 @default.
- W4365455576 cites W2151122254 @default.
- W4365455576 cites W2151899848 @default.
- W4365455576 cites W2157248748 @default.
- W4365455576 cites W2158804744 @default.
- W4365455576 cites W2159038577 @default.
- W4365455576 cites W2160378127 @default.
- W4365455576 cites W2169555805 @default.
- W4365455576 cites W2170027074 @default.
- W4365455576 cites W2174027288 @default.
- W4365455576 cites W2174231125 @default.
- W4365455576 cites W2315924176 @default.
- W4365455576 cites W2328180398 @default.
- W4365455576 cites W2345476689 @default.
- W4365455576 cites W2479687279 @default.
- W4365455576 cites W2520898329 @default.
- W4365455576 cites W2604329751 @default.
- W4365455576 cites W2612079819 @default.
- W4365455576 cites W2621778125 @default.
- W4365455576 cites W2888678189 @default.
- W4365455576 cites W2930660192 @default.
- W4365455576 cites W2941072767 @default.
- W4365455576 cites W2949086752 @default.
- W4365455576 cites W2949447642 @default.
- W4365455576 cites W2950682063 @default.
- W4365455576 cites W2980032774 @default.
- W4365455576 cites W3017049640 @default.
- W4365455576 cites W3098477092 @default.
- W4365455576 cites W3121038268 @default.
- W4365455576 cites W3128198169 @default.
- W4365455576 cites W3177800059 @default.
- W4365455576 cites W3211544104 @default.
- W4365455576 cites W4223939585 @default.
- W4365455576 cites W4240865931 @default.
- W4365455576 cites W4293484779 @default.
- W4365455576 cites W2415258310 @default.
- W4365455576 cites W3170389254 @default.
- W4365455576 doi "https://doi.org/10.3389/fmicb.2023.1152586" @default.
- W4365455576 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37125197" @default.
- W4365455576 hasPublicationYear "2023" @default.
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