FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4365483672> ?p ?o ?g. }

• W4365483672 abstract "Translocation analysis using fluorescence in situ hybridization (FISH) is the method of choice for dose assessment in case of chronic or past exposures to ionizing radiation. Although it is a widespread technique, unlike dicentrics, the number of FISH-based inter-laboratory comparisons is small. For this reason, although the current Running the European Network of Biological and Physical retrospective Dosimetry (RENEB) inter-laboratory comparison 2021 was designed as a fast response to a real emergency scenario, it was considered a good opportunity to perform an inter-laboratory comparison using the FISH technique to gain further experience. The Bundeswehr Institute of Radiobiology provided peripheral blood samples from one healthy human volunteer. Three test samples were irradiated with blinded doses of 0, 1.2, and 3.5 Gy, respectively. Samples were then sent to the seven participating laboratories. The FISH technique was applied according to the standard procedure of each laboratory. Both, the frequency of translocations and the estimated dose for each sample were sent to the coordinator using a special scoring sheet for FISH. All participants sent their results in due time. However, although it was initially requested to send the results based on the full analysis, evaluating 500 equivalent cells, most laboratories only sent the results based on triage, with a smaller number of analyzed cells. In the triage analysis, there was great heterogeneity in the number of equivalent cells scored. On the contrary, for the full analysis, this number was more homogeneous. For all three samples, one laboratory showed outlier yields compared to the other laboratories. Excluding these results, in the triage analysis, the frequency of translocations in sample no. 1 ranged from 0 to 0.013 translocations per cell, and for samples no. 2 and no. 3 the genomic mean frequency were 0.27 ± 0.03 and 1.47 ± 0.14, with a coefficient of variation of 0.29 and 0.23 respectively. Considering only results obtained in the triage analysis for sample no. 1, all laboratories, except one, classified this sample as the non-irradiated one. For sample no. 2, excluding the outlier value, the mean reported dose was 1.74 ± 0.16 Gy indicating a mean deviation of about 0.5 Gy to the delivered dose of 1.2 Gy. For sample no. 3 the mean dose estimated was 4.21 ± 0.21 Gy indicating a mean deviation of about 0.7 Gy to the delivered dose of 3.5 Gy. In the frame of RENEB, this is the second FISH-based inter-laboratory comparison. The whole exercise was planned as a response to an emergency, therefore, a triage analysis was requested for all the biomarkers except for FISH. Although a full analysis was initially requested for FISH, most of the laboratories reported only a triage-based result. The main reason is that it was not clearly stated what was required before starting the exercise. Results show that most of the laboratories successfully discriminated unexposed and irradiated samples from each other without any overlap. A good agreement in the observed frequencies of translocations was observed but there was a tendency to overestimate the delivered doses. Efforts to improve the harmonization of this technique and subsequent exercises to elucidate the reason for this trend should be promoted." @default.
• W4365483672 created "2023-04-15" @default.
• W4365483672 creator A5001818110 @default.
• W4365483672 creator A5008919275 @default.
• W4365483672 creator A5008964744 @default.
• W4365483672 creator A5014957701 @default.
• W4365483672 creator A5017342881 @default.
• W4365483672 creator A5028798185 @default.
• W4365483672 creator A5030200918 @default.
• W4365483672 creator A5032994316 @default.
• W4365483672 creator A5034138334 @default.
• W4365483672 creator A5036129450 @default.
• W4365483672 creator A5048709085 @default.
• W4365483672 creator A5050275493 @default.
• W4365483672 creator A5051784102 @default.
• W4365483672 creator A5053707443 @default.
• W4365483672 creator A5074406651 @default.
• W4365483672 creator A5076184251 @default.
• W4365483672 creator A5076766734 @default.
• W4365483672 creator A5076802927 @default.
• W4365483672 creator A5079974992 @default.
• W4365483672 creator A5087319048 @default.
• W4365483672 creator A5087842073 @default.
• W4365483672 creator A5090555024 @default.
• W4365483672 date "2023-04-13" @default.
• W4365483672 modified "2023-10-18" @default.
• W4365483672 title "RENEB Inter-Laboratory Comparison 2021: The FISH-Based Translocation Assay" @default.
• W4365483672 cites W1919497285 @default.
• W4365483672 cites W1946597462 @default.
• W4365483672 cites W2023055119 @default.
• W4365483672 cites W2025912239 @default.
• W4365483672 cites W2046815582 @default.
• W4365483672 cites W2058892252 @default.
• W4365483672 cites W2064489306 @default.
• W4365483672 cites W2094798673 @default.
• W4365483672 cites W2108994753 @default.
• W4365483672 cites W2116184620 @default.
• W4365483672 cites W2126295706 @default.
• W4365483672 cites W2141403400 @default.
• W4365483672 cites W2160932916 @default.
• W4365483672 cites W2162808964 @default.
• W4365483672 cites W2170963316 @default.
• W4365483672 cites W2179955571 @default.
• W4365483672 cites W2312360776 @default.
• W4365483672 cites W2332752560 @default.
• W4365483672 cites W2333905165 @default.
• W4365483672 cites W2500906989 @default.
• W4365483672 cites W2517224657 @default.
• W4365483672 cites W2528979342 @default.
• W4365483672 cites W2790288013 @default.
• W4365483672 cites W4318945473 @default.
• W4365483672 cites W941095877 @default.
• W4365483672 doi "https://doi.org/10.1667/rade-22-00203.1" @default.
• W4365483672 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37057978" @default.
• W4365483672 hasPublicationYear "2023" @default.
• W4365483672 type Work @default.
• W4365483672 citedByCount "3" @default.
• W4365483672 countsByYear W43654836722023 @default.
• W4365483672 crossrefType "journal-article" @default.
• W4365483672 hasAuthorship W4365483672A5001818110 @default.
• W4365483672 hasAuthorship W4365483672A5008919275 @default.
• W4365483672 hasAuthorship W4365483672A5008964744 @default.
• W4365483672 hasAuthorship W4365483672A5014957701 @default.
• W4365483672 hasAuthorship W4365483672A5017342881 @default.
• W4365483672 hasAuthorship W4365483672A5028798185 @default.
• W4365483672 hasAuthorship W4365483672A5030200918 @default.
• W4365483672 hasAuthorship W4365483672A5032994316 @default.
• W4365483672 hasAuthorship W4365483672A5034138334 @default.
• W4365483672 hasAuthorship W4365483672A5036129450 @default.
• W4365483672 hasAuthorship W4365483672A5048709085 @default.
• W4365483672 hasAuthorship W4365483672A5050275493 @default.
• W4365483672 hasAuthorship W4365483672A5051784102 @default.
• W4365483672 hasAuthorship W4365483672A5053707443 @default.
• W4365483672 hasAuthorship W4365483672A5074406651 @default.
• W4365483672 hasAuthorship W4365483672A5076184251 @default.
• W4365483672 hasAuthorship W4365483672A5076766734 @default.
• W4365483672 hasAuthorship W4365483672A5076802927 @default.
• W4365483672 hasAuthorship W4365483672A5079974992 @default.
• W4365483672 hasAuthorship W4365483672A5087319048 @default.
• W4365483672 hasAuthorship W4365483672A5087842073 @default.
• W4365483672 hasAuthorship W4365483672A5090555024 @default.
• W4365483672 hasConcept C104317684 @default.
• W4365483672 hasConcept C111337013 @default.
• W4365483672 hasConcept C121332964 @default.
• W4365483672 hasConcept C18231593 @default.
• W4365483672 hasConcept C185544564 @default.
• W4365483672 hasConcept C194828623 @default.
• W4365483672 hasConcept C19527891 @default.
• W4365483672 hasConcept C203014093 @default.
• W4365483672 hasConcept C2777120189 @default.
• W4365483672 hasConcept C2777542201 @default.
• W4365483672 hasConcept C2779254040 @default.
• W4365483672 hasConcept C2909208804 @default.
• W4365483672 hasConcept C2989005 @default.
• W4365483672 hasConcept C3017910909 @default.
• W4365483672 hasConcept C30481170 @default.
• W4365483672 hasConcept C505870484 @default.
• W4365483672 hasConcept C55493867 @default.
• W4365483672 hasConcept C66882249 @default.
• W4365483672 hasConcept C71924100 @default.

• next