Matches in SemOpenAlex for { <https://semopenalex.org/work/W4365510059> ?p ?o ?g. }
- W4365510059 endingPage "7298" @default.
- W4365510059 startingPage "7298" @default.
- W4365510059 abstract "Low serum levels of 1α, 25-dihydroxyvitamin D3 (VD3) are associated with a higher mortality in trauma patients with sepsis or ARDS. However, the molecular mechanisms behind this observation are not yet understood. VD3 is known to stimulate lung maturity, alveolar type II cell differentiation, or pulmonary surfactant synthesis and guides epithelial defense during infection. In this study, we investigated the impact of VD3 on the alveolar–capillary barrier in a co-culture model of alveolar epithelial cells and microvascular endothelial cells respectively in the individual cell types. After stimulation with bacterial LPS (lipopolysaccharide), gene expression of inflammatory cytokines, surfactant proteins, transport proteins, antimicrobial peptide, and doublecortin-like kinase 1 (DCLK1) were analyzed by real-time PCR, while corresponding proteins were evaluated by ELISA, immune-fluorescence, or Western blot. The effect of VD3 on the intracellular protein composition in H441 cells was analyzed by quantitative liquid chromatography-mass spectrometry-based proteomics. VD3 effectively protected the alveolar–capillary barrier against LPS treatment, as indicated by TEER measurement and morphological assessment. VD3 did not inhibit the IL-6 secretion by H441 and OEC but restricted the diffusion of IL-6 to the epithelial compartment. Further, VD3 could significantly suppress the surfactant protein A expression induced in the co-culture system by LPS treatment. VD3 induced high levels of the antimicrobial peptide LL-37, which counteracted effects by LPS and strengthened the barrier. Quantitative proteomics identified VD3-dependent protein abundance changes ranging from constitutional extracellular matrix components and surfactant-associated proteins to immune-regulatory molecules. DCLK1, as a newly described target molecule for VD3, was prominently stimulated by VD3 (10 nM) and seems to influence the alveolar–epithelial cell barrier and regeneration." @default.
- W4365510059 created "2023-04-15" @default.
- W4365510059 creator A5015647125 @default.
- W4365510059 creator A5016648966 @default.
- W4365510059 creator A5032216035 @default.
- W4365510059 creator A5032935690 @default.
- W4365510059 creator A5055531816 @default.
- W4365510059 creator A5058842854 @default.
- W4365510059 creator A5059498166 @default.
- W4365510059 creator A5066052190 @default.
- W4365510059 creator A5081714031 @default.
- W4365510059 creator A5083280015 @default.
- W4365510059 creator A5090131235 @default.
- W4365510059 date "2023-04-14" @default.
- W4365510059 modified "2023-10-14" @default.
- W4365510059 title "Alveolar–Capillary Barrier Protection In Vitro: Lung Cell Type-Specific Effects and Molecular Mechanisms Induced by 1α, 25-Dihydroxyvitamin D3" @default.
- W4365510059 cites W100256784 @default.
- W4365510059 cites W1526837316 @default.
- W4365510059 cites W1548718770 @default.
- W4365510059 cites W1582276041 @default.
- W4365510059 cites W1761203024 @default.
- W4365510059 cites W1918296015 @default.
- W4365510059 cites W1929210191 @default.
- W4365510059 cites W1931163389 @default.
- W4365510059 cites W1963614683 @default.
- W4365510059 cites W1964243778 @default.
- W4365510059 cites W1964391821 @default.
- W4365510059 cites W1967069761 @default.
- W4365510059 cites W1970909871 @default.
- W4365510059 cites W1970966716 @default.
- W4365510059 cites W1974587361 @default.
- W4365510059 cites W1976133370 @default.
- W4365510059 cites W1979508193 @default.
- W4365510059 cites W1983475529 @default.
- W4365510059 cites W2000142592 @default.
- W4365510059 cites W2005604209 @default.
- W4365510059 cites W2006986579 @default.
- W4365510059 cites W2010061535 @default.
- W4365510059 cites W2011710911 @default.
- W4365510059 cites W2013252828 @default.
- W4365510059 cites W2014699342 @default.
- W4365510059 cites W2016317398 @default.
- W4365510059 cites W20182063 @default.
- W4365510059 cites W2020012047 @default.
- W4365510059 cites W2023010129 @default.
- W4365510059 cites W2026644677 @default.
- W4365510059 cites W2029675812 @default.
- W4365510059 cites W2042577267 @default.
- W4365510059 cites W2044310665 @default.
- W4365510059 cites W2047680613 @default.
- W4365510059 cites W2047688409 @default.
- W4365510059 cites W2048280438 @default.
- W4365510059 cites W2048950527 @default.
- W4365510059 cites W2051244361 @default.
- W4365510059 cites W2056315961 @default.
- W4365510059 cites W2062097568 @default.
- W4365510059 cites W2062345026 @default.
- W4365510059 cites W2065967553 @default.
- W4365510059 cites W2073740870 @default.
- W4365510059 cites W2075194927 @default.
- W4365510059 cites W2082495239 @default.
- W4365510059 cites W2082757239 @default.
- W4365510059 cites W2084126190 @default.
- W4365510059 cites W2085124463 @default.
- W4365510059 cites W2103017472 @default.
- W4365510059 cites W2106606013 @default.
- W4365510059 cites W2107941234 @default.
- W4365510059 cites W2110256992 @default.
- W4365510059 cites W2117224932 @default.
- W4365510059 cites W2121348586 @default.
- W4365510059 cites W2122662583 @default.
- W4365510059 cites W2125663148 @default.
- W4365510059 cites W2127675927 @default.
- W4365510059 cites W2131264460 @default.
- W4365510059 cites W2134495681 @default.
- W4365510059 cites W2136083733 @default.
- W4365510059 cites W2136619797 @default.
- W4365510059 cites W2141978072 @default.
- W4365510059 cites W2143657892 @default.
- W4365510059 cites W2145082642 @default.
- W4365510059 cites W2157508179 @default.
- W4365510059 cites W2164133446 @default.
- W4365510059 cites W2164138095 @default.
- W4365510059 cites W2168945351 @default.
- W4365510059 cites W2181566983 @default.
- W4365510059 cites W2191813810 @default.
- W4365510059 cites W2204037601 @default.
- W4365510059 cites W2224056471 @default.
- W4365510059 cites W2416372391 @default.
- W4365510059 cites W2463195069 @default.
- W4365510059 cites W2577816239 @default.
- W4365510059 cites W2728214347 @default.
- W4365510059 cites W2745730349 @default.
- W4365510059 cites W2753103551 @default.
- W4365510059 cites W2768562036 @default.
- W4365510059 cites W2773972374 @default.
- W4365510059 cites W2909263085 @default.
- W4365510059 cites W2911784321 @default.