FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4365511660> ?p ?o ?g. }

• W4365511660 endingPage "LB107" @default.
• W4365511660 startingPage "LB107" @default.
• W4365511660 abstract "Abstract Cervical cancer is one of the most common cancers in women. Despite progress in prevention through Human Papilloma Virus (HPV) vaccination and success in early detection of cervical cancer through cytologic screening and HPV detection, there remains an unequal cervical cancer burden in low-resource settings, both in developed and developing countries. We have previously shown that the CervicalMethDx test can provide a Cervical Intraepithelial Neoplasia (CIN) grade 2-3 risk score, by assessing DNA methylation in a panel of three human genes (ZNF516, FKBP6 and INTS1) in samples from the United States (US), Puerto Rico, and Chile. We now tested the performance of the CervicalMethDx test on HPV-positive CIN2 and CIN3 cases (n=113) from Honduras collected from participants in the ESTAMPA clinical trial (NCT01881659). ESTAMPA (EStudio multicéntrico de TAMizaje y triaje de cáncer de cuello uterino con pruebas del virus del PApiloma humano; Spanish acronym) is a multicentric study of cervical cancer screening with HPV testing and assessment of triage methods in Latin America, which has accrued close to 50,000 participants from twelve recruitment centers in nine Latin American countries since 2013. We hypothesized that the CervicalMethDx test can identify HPV positive women most likely to be diagnosed with CIN grades 2 and 3 by anatomic pathologists, before they are referred to colposcopy-driven biopsies. We assessed DNA methylation by quantitative Real Time Methylation Specific PCR (qMSP) analysis of sodium bisulfite-modified genomic DNA. Primers and probes were previously designed to specifically amplify the promoters of the 3 genes of interest and the promoter of a reference gene, β-actin, to assess DNA input. We performed blinded retrospective studies on well-characterized clinical samples in PreservCyt sample transport media (ThinPrep, Hologic), comparing DNA methylation levels in samples from Honduras and 88 HPV-positive previously tested US samples with No Intraepithelial Lesions or Malignancy (NILM), as controls. Our results showed that the CervicalMethDx test can correctly classify 96% of CIN2 (n=62) samples with 92% Sensitivity, 98% Specificity, and an AUC of 0.96 as well as 97% of CIN3 (n=51) samples with 96% Sensitivity, 98% Specificity, and an AUC of 0.97. Moreover, the assay correctly classified 96% of CIN2-CIN3 samples combined (n=113) with 95% Sensitivity, 98% Specificity, and AUC of 0.97, when compared to samples with NILM (n=88). Our results suggest that the CervicalMethDx test is a new and valuable tool to stratify HPV positive women prior to colposcopy-driven biopsies in developed countries and ablative treatment in developing countries, most of which are unnecessary. These results warrant further evaluation of the CervicalMethDX test in prospective, population-based studies, assessing the use of precision DNA methylation algorithms to triage HPV positive women before referral to colposcopy-driven biopsies or ablative treatments in cervical cancer screening clinics world-wide. Citation Format: Laura Palmieri, Fernando Zamuner, Yessy Cabrera, Jafet Ortiz-Quintero, Dieila Giomo De Lima, Ana Purcell-Wiltz, Amanda García-Negrón, Ashley Ramos-López, Mariana Brait, David Sidransky, Annabelle Ferrera, Rafael E. Guerrero-Preston. A precision DNA methylation test to triage HPV positive women before referral to colposcopy-driven biopsies or ablative treatment in cervical cancer screening clinics worldwide [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 2 (Clinical Trials and Late-Breaking Research); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(8_Suppl):Abstract nr LB107." @default.
• W4365511660 created "2023-04-15" @default.
• W4365511660 creator A5010778147 @default.
• W4365511660 creator A5016484824 @default.
• W4365511660 creator A5017828901 @default.
• W4365511660 creator A5018963012 @default.
• W4365511660 creator A5027327944 @default.
• W4365511660 creator A5037832079 @default.
• W4365511660 creator A5048533064 @default.
• W4365511660 creator A5049209796 @default.
• W4365511660 creator A5053907228 @default.
• W4365511660 creator A5055159944 @default.
• W4365511660 creator A5060824460 @default.
• W4365511660 creator A5082055232 @default.
• W4365511660 date "2023-04-14" @default.
• W4365511660 modified "2023-09-29" @default.
• W4365511660 title "Abstract LB107: A precision DNA methylation test to triage HPV positive women before referral to colposcopy-driven biopsies or ablative treatment in cervical cancer screening clinics worldwide" @default.
• W4365511660 doi "https://doi.org/10.1158/1538-7445.am2023-lb107" @default.
• W4365511660 hasPublicationYear "2023" @default.
• W4365511660 type Work @default.
• W4365511660 citedByCount "0" @default.
• W4365511660 crossrefType "journal-article" @default.
• W4365511660 hasAuthorship W4365511660A5010778147 @default.
• W4365511660 hasAuthorship W4365511660A5016484824 @default.
• W4365511660 hasAuthorship W4365511660A5017828901 @default.
• W4365511660 hasAuthorship W4365511660A5018963012 @default.
• W4365511660 hasAuthorship W4365511660A5027327944 @default.
• W4365511660 hasAuthorship W4365511660A5037832079 @default.
• W4365511660 hasAuthorship W4365511660A5048533064 @default.
• W4365511660 hasAuthorship W4365511660A5049209796 @default.
• W4365511660 hasAuthorship W4365511660A5053907228 @default.
• W4365511660 hasAuthorship W4365511660A5055159944 @default.
• W4365511660 hasAuthorship W4365511660A5060824460 @default.
• W4365511660 hasAuthorship W4365511660A5082055232 @default.
• W4365511660 hasConcept C104317684 @default.
• W4365511660 hasConcept C121608353 @default.
• W4365511660 hasConcept C126322002 @default.
• W4365511660 hasConcept C131872663 @default.
• W4365511660 hasConcept C143998085 @default.
• W4365511660 hasConcept C150194340 @default.
• W4365511660 hasConcept C190727270 @default.
• W4365511660 hasConcept C194828623 @default.
• W4365511660 hasConcept C2776117191 @default.
• W4365511660 hasConcept C2776135927 @default.
• W4365511660 hasConcept C2777120189 @default.
• W4365511660 hasConcept C2777343196 @default.
• W4365511660 hasConcept C2778220009 @default.
• W4365511660 hasConcept C2779742066 @default.
• W4365511660 hasConcept C29456083 @default.
• W4365511660 hasConcept C2993522905 @default.
• W4365511660 hasConcept C512399662 @default.
• W4365511660 hasConcept C55493867 @default.
• W4365511660 hasConcept C71924100 @default.
• W4365511660 hasConcept C86803240 @default.
• W4365511660 hasConceptScore W4365511660C104317684 @default.
• W4365511660 hasConceptScore W4365511660C121608353 @default.
• W4365511660 hasConceptScore W4365511660C126322002 @default.
• W4365511660 hasConceptScore W4365511660C131872663 @default.
• W4365511660 hasConceptScore W4365511660C143998085 @default.
• W4365511660 hasConceptScore W4365511660C150194340 @default.
• W4365511660 hasConceptScore W4365511660C190727270 @default.
• W4365511660 hasConceptScore W4365511660C194828623 @default.
• W4365511660 hasConceptScore W4365511660C2776117191 @default.
• W4365511660 hasConceptScore W4365511660C2776135927 @default.
• W4365511660 hasConceptScore W4365511660C2777120189 @default.
• W4365511660 hasConceptScore W4365511660C2777343196 @default.
• W4365511660 hasConceptScore W4365511660C2778220009 @default.
• W4365511660 hasConceptScore W4365511660C2779742066 @default.
• W4365511660 hasConceptScore W4365511660C29456083 @default.
• W4365511660 hasConceptScore W4365511660C2993522905 @default.
• W4365511660 hasConceptScore W4365511660C512399662 @default.
• W4365511660 hasConceptScore W4365511660C55493867 @default.
• W4365511660 hasConceptScore W4365511660C71924100 @default.
• W4365511660 hasConceptScore W4365511660C86803240 @default.
• W4365511660 hasIssue "8_Supplement" @default.
• W4365511660 hasLocation W43655116601 @default.
• W4365511660 hasOpenAccess W4365511660 @default.
• W4365511660 hasPrimaryLocation W43655116601 @default.
• W4365511660 hasRelatedWork W2028708967 @default.
• W4365511660 hasRelatedWork W2039633905 @default.
• W4365511660 hasRelatedWork W2085678497 @default.
• W4365511660 hasRelatedWork W2395108346 @default.
• W4365511660 hasRelatedWork W2518434407 @default.
• W4365511660 hasRelatedWork W2530876345 @default.
• W4365511660 hasRelatedWork W2759875907 @default.
• W4365511660 hasRelatedWork W2903748704 @default.
• W4365511660 hasRelatedWork W3132092335 @default.
• W4365511660 hasRelatedWork W2784924283 @default.
• W4365511660 hasVolume "83" @default.
• W4365511660 isParatext "false" @default.
• W4365511660 isRetracted "false" @default.
• W4365511660 workType "article" @default.