FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4365514502> ?p ?o ?g. }

• W4365514502 endingPage "908" @default.
• W4365514502 startingPage "908" @default.
• W4365514502 abstract "Cytosine base editors (CBEs) and adenine base editors (ABEs) are recently developed CRISPR-mediated genome-editing tools that do not introduce double-strand breaks. In this study, five ABEs, ABE7.10, ABEmax, NG-ABEmax, ABE8e and NG-ABE8e, were used to generate A-to-G (T-to-C) conversions in five genome loci in porcine fetal fibroblasts (PFFs). Variable yet appreciable editing efficiencies and variable activity windows were observed in these targeting regions via these five editors. The strategy of two sgRNAs in one vector exhibited superior editing efficiency to that of using two separate sgRNA expression vectors. ABE-mediated start-codon mutation in APOE silenced its expression of protein and, unexpectedly, eliminated the vast majority of its mRNA. No off-target DNA site was detected for these editors. Substantial off-target RNA events were present in the ABE-edited cells, but no KEGG pathway was found to be significantly enriched. Our study supports that ABEs are powerful tools for A-to-G (T-to-C) point-mutation modification in porcine cells." @default.
• W4365514502 created "2023-04-15" @default.
• W4365514502 creator A5004183272 @default.
• W4365514502 creator A5011447814 @default.
• W4365514502 creator A5039941759 @default.
• W4365514502 creator A5064274150 @default.
• W4365514502 creator A5069921439 @default.
• W4365514502 creator A5076256981 @default.
• W4365514502 date "2023-04-13" @default.
• W4365514502 modified "2023-09-26" @default.
• W4365514502 title "Highly Efficient A-to-G Editing in PFFs via Multiple ABEs" @default.
• W4365514502 cites W1845272621 @default.
• W4365514502 cites W1983015423 @default.
• W4365514502 cites W1987053150 @default.
• W4365514502 cites W1991339921 @default.
• W4365514502 cites W2026592619 @default.
• W4365514502 cites W2035618305 @default.
• W4365514502 cites W2045435533 @default.
• W4365514502 cites W2070172761 @default.
• W4365514502 cites W2082662254 @default.
• W4365514502 cites W2105583461 @default.
• W4365514502 cites W2123828036 @default.
• W4365514502 cites W2138207763 @default.
• W4365514502 cites W2147736027 @default.
• W4365514502 cites W2179438025 @default.
• W4365514502 cites W2336828812 @default.
• W4365514502 cites W2506364686 @default.
• W4365514502 cites W2766599608 @default.
• W4365514502 cites W2805505209 @default.
• W4365514502 cites W2883697525 @default.
• W4365514502 cites W2883893004 @default.
• W4365514502 cites W2884007288 @default.
• W4365514502 cites W2885661865 @default.
• W4365514502 cites W2897295193 @default.
• W4365514502 cites W2919055546 @default.
• W4365514502 cites W2939749480 @default.
• W4365514502 cites W2946692293 @default.
• W4365514502 cites W2948336721 @default.
• W4365514502 cites W2951233129 @default.
• W4365514502 cites W2981762194 @default.
• W4365514502 cites W2985918969 @default.
• W4365514502 cites W2990758262 @default.
• W4365514502 cites W3012327875 @default.
• W4365514502 cites W3023659887 @default.
• W4365514502 cites W3039159245 @default.
• W4365514502 cites W3039582431 @default.
• W4365514502 cites W3046607175 @default.
• W4365514502 cites W3089035168 @default.
• W4365514502 cites W3093469725 @default.
• W4365514502 cites W3112351068 @default.
• W4365514502 cites W3121977414 @default.
• W4365514502 cites W3134349365 @default.
• W4365514502 cites W3134889339 @default.
• W4365514502 cites W3173485772 @default.
• W4365514502 cites W3194942572 @default.
• W4365514502 cites W3199505060 @default.
• W4365514502 cites W3200094259 @default.
• W4365514502 cites W4210287411 @default.
• W4365514502 cites W4226219421 @default.
• W4365514502 cites W4226342186 @default.
• W4365514502 cites W4229335197 @default.
• W4365514502 cites W4285682548 @default.
• W4365514502 cites W4305015968 @default.
• W4365514502 cites W4308149002 @default.
• W4365514502 doi "https://doi.org/10.3390/genes14040908" @default.
• W4365514502 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37107666" @default.
• W4365514502 hasPublicationYear "2023" @default.
• W4365514502 type Work @default.
• W4365514502 citedByCount "0" @default.
• W4365514502 crossrefType "journal-article" @default.
• W4365514502 hasAuthorship W4365514502A5004183272 @default.
• W4365514502 hasAuthorship W4365514502A5011447814 @default.
• W4365514502 hasAuthorship W4365514502A5039941759 @default.
• W4365514502 hasAuthorship W4365514502A5064274150 @default.
• W4365514502 hasAuthorship W4365514502A5069921439 @default.
• W4365514502 hasAuthorship W4365514502A5076256981 @default.
• W4365514502 hasBestOaLocation W43655145021 @default.
• W4365514502 hasConcept C104317684 @default.
• W4365514502 hasConcept C132455925 @default.
• W4365514502 hasConcept C141231307 @default.
• W4365514502 hasConcept C144501496 @default.
• W4365514502 hasConcept C153911025 @default.
• W4365514502 hasConcept C176944494 @default.
• W4365514502 hasConcept C189819185 @default.
• W4365514502 hasConcept C20580545 @default.
• W4365514502 hasConcept C2776023528 @default.
• W4365514502 hasConcept C501734568 @default.
• W4365514502 hasConcept C54355233 @default.
• W4365514502 hasConcept C552990157 @default.
• W4365514502 hasConcept C67705224 @default.
• W4365514502 hasConcept C70721500 @default.
• W4365514502 hasConcept C86803240 @default.
• W4365514502 hasConcept C98108389 @default.
• W4365514502 hasConceptScore W4365514502C104317684 @default.
• W4365514502 hasConceptScore W4365514502C132455925 @default.
• W4365514502 hasConceptScore W4365514502C141231307 @default.
• W4365514502 hasConceptScore W4365514502C144501496 @default.
• W4365514502 hasConceptScore W4365514502C153911025 @default.

• next