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- W4366067874 abstract "<h3>BACKGROUND</h3> Microbiological investigations of vitreous fluid (VF) and aqueous humour (AH) specimens have often failed to detect the infecting agent in infectious endophthalmitis, resulting in a clinical dilemma regarding therapy. In this study, the polymerase chain reaction (PCR) was evaluated in the diagnosis of bacterial and<i>Propionibacterium acnes</i> endophthalmitis. <h3>METHODS</h3> 58 intraocular specimens (30 VF and 28 AH) from 55 cases of endophthalmitis and 20 specimens (14 VF and 6 AH) as controls from non-infective disorders were processed for microbiological investigations. Nested PCR directed at the 16S rDNA using universal primers for eubacterial genome was done. PCR for <i>P acnes</i> was performed on specimens microbiologically negative by conventional techniques but eubacterial genome positive. <h3>RESULTS</h3> Of the 20 controls from non-infective cases, one (5%) was positive using eubacterial primers and none with<i>P acnes</i> primers. PCR for eubacterial genome showed 100% correlation with 20 (34.5%) bacteriologically positive specimens. Eubacterial genome, was detected in 17 (44.7%) of 38 bacteriologically negative specimens and nine (52.9%) out of the 17 were positive for <i>P acnes</i> genome. Among the 21 eubacterial PCR negative specimens, seven were fungus positive. By inclusion of PCR, microbiologically positive specimens increased from 46.5% to 75.8%. PCR on AH was as sensitive as that on VF for the detection of both eubacterial and the <i>P acnes</i> genome. <h3>CONCLUSION</h3> PCR performed on AH and VF is a reliable tool for the diagnosis of bacterial and <i>P acnes</i>endophthalmitis particularly in smear and culture negative specimens." @default.
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- W4366067874 date "1998-09-01" @default.
- W4366067874 modified "2023-10-10" @default.
- W4366067874 title "Polymerase chain reaction in the diagnosis of bacterial endophthalmitis" @default.
- W4366067874 doi "https://doi.org/10.1136/bjo.82.9.1078" @default.
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