Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366085266> ?p ?o ?g. }
- W4366085266 endingPage "104724" @default.
- W4366085266 startingPage "104724" @default.
- W4366085266 abstract "Bacterial pathogens like Mycobacterium tuberculosis (Mtb) employ transcription factors to adapt their physiology to the diverse environments within their host. CarD is a conserved bacterial transcription factor that is essential for viability in Mtb. Unlike classical transcription factors that recognize promoters by binding to specific DNA sequence motifs, CarD binds directly to the RNA polymerase to stabilize the open complex intermediate (RPo) during transcription initiation. We previously showed using RNA-sequencing that CarD is capable of both activating and repressing transcription in vivo. However, it is unknown how CarD achieves promoter-specific regulatory outcomes in Mtb despite binding indiscriminate of DNA sequence. We propose a model where CarD's regulatory outcome depends on the promoter's basal RPo stability and test this model using in vitro transcription from a panel of promoters with varying levels of RPo stability. We show that CarD directly activates full-length transcript production from the Mtb ribosomal RNA promoter rrnAP3 (AP3) and that the degree of transcription activation by CarD is negatively correlated with RPo stability. Using targeted mutations in the extended -10 and discriminator region of AP3, we show that CarD directly represses transcription from promoters that form relatively stable RPo. DNA supercoiling also influenced RPo stability and affected the direction of CarD regulation, indicating that the outcome of CarD activity can be regulated by factors beyond promoter sequence. Our results provide experimental evidence for how RNA polymerase-binding transcription factors like CarD can exert specific regulatory outcomes based on the kinetic properties of a promoter." @default.
- W4366085266 created "2023-04-19" @default.
- W4366085266 creator A5030633317 @default.
- W4366085266 creator A5079849215 @default.
- W4366085266 date "2023-06-01" @default.
- W4366085266 modified "2023-09-26" @default.
- W4366085266 title "Transcription regulation by CarD in mycobacteria is guided by basal promoter kinetics" @default.
- W4366085266 cites W1581263435 @default.
- W4366085266 cites W1762025233 @default.
- W4366085266 cites W1912050508 @default.
- W4366085266 cites W1945781696 @default.
- W4366085266 cites W1952298091 @default.
- W4366085266 cites W1972587297 @default.
- W4366085266 cites W1975958238 @default.
- W4366085266 cites W1976222270 @default.
- W4366085266 cites W1994265058 @default.
- W4366085266 cites W1999434982 @default.
- W4366085266 cites W2018349754 @default.
- W4366085266 cites W2028812045 @default.
- W4366085266 cites W2034500668 @default.
- W4366085266 cites W2036758334 @default.
- W4366085266 cites W2042329136 @default.
- W4366085266 cites W2043761418 @default.
- W4366085266 cites W2046228091 @default.
- W4366085266 cites W2052660926 @default.
- W4366085266 cites W2065015169 @default.
- W4366085266 cites W2074293686 @default.
- W4366085266 cites W2075716499 @default.
- W4366085266 cites W2083632051 @default.
- W4366085266 cites W2103314018 @default.
- W4366085266 cites W2105786825 @default.
- W4366085266 cites W2106494572 @default.
- W4366085266 cites W2109467694 @default.
- W4366085266 cites W2111441093 @default.
- W4366085266 cites W2113722430 @default.
- W4366085266 cites W2131271579 @default.
- W4366085266 cites W2132522708 @default.
- W4366085266 cites W2138068417 @default.
- W4366085266 cites W2138207763 @default.
- W4366085266 cites W2143726250 @default.
- W4366085266 cites W2146120613 @default.
- W4366085266 cites W2154583792 @default.
- W4366085266 cites W2155347381 @default.
- W4366085266 cites W2168639903 @default.
- W4366085266 cites W2168676463 @default.
- W4366085266 cites W2171451207 @default.
- W4366085266 cites W2179438025 @default.
- W4366085266 cites W2314357684 @default.
- W4366085266 cites W2337253598 @default.
- W4366085266 cites W2434616321 @default.
- W4366085266 cites W2461585639 @default.
- W4366085266 cites W2552715737 @default.
- W4366085266 cites W2560522998 @default.
- W4366085266 cites W2569618521 @default.
- W4366085266 cites W2597915083 @default.
- W4366085266 cites W2735563253 @default.
- W4366085266 cites W2742975915 @default.
- W4366085266 cites W2792532307 @default.
- W4366085266 cites W2800367049 @default.
- W4366085266 cites W2802032176 @default.
- W4366085266 cites W2850292602 @default.
- W4366085266 cites W2891742319 @default.
- W4366085266 cites W2901183331 @default.
- W4366085266 cites W2910191461 @default.
- W4366085266 cites W2922948832 @default.
- W4366085266 cites W2936521062 @default.
- W4366085266 cites W2946228571 @default.
- W4366085266 cites W2952565690 @default.
- W4366085266 cites W2963716098 @default.
- W4366085266 cites W2964849163 @default.
- W4366085266 cites W2996650908 @default.
- W4366085266 cites W3001486028 @default.
- W4366085266 cites W3012214096 @default.
- W4366085266 cites W3045966928 @default.
- W4366085266 cites W3096375668 @default.
- W4366085266 cites W3098407573 @default.
- W4366085266 cites W3108310633 @default.
- W4366085266 cites W3176739454 @default.
- W4366085266 cites W3185949378 @default.
- W4366085266 cites W3204646384 @default.
- W4366085266 cites W4200135473 @default.
- W4366085266 cites W4220913710 @default.
- W4366085266 cites W4280527591 @default.
- W4366085266 doi "https://doi.org/10.1016/j.jbc.2023.104724" @default.
- W4366085266 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37075846" @default.
- W4366085266 hasPublicationYear "2023" @default.
- W4366085266 type Work @default.
- W4366085266 citedByCount "0" @default.
- W4366085266 crossrefType "journal-article" @default.
- W4366085266 hasAuthorship W4366085266A5030633317 @default.
- W4366085266 hasAuthorship W4366085266A5079849215 @default.
- W4366085266 hasBestOaLocation W43660852661 @default.
- W4366085266 hasConcept C101762097 @default.
- W4366085266 hasConcept C104317684 @default.
- W4366085266 hasConcept C132376346 @default.
- W4366085266 hasConcept C138885662 @default.
- W4366085266 hasConcept C144825837 @default.
- W4366085266 hasConcept C150194340 @default.