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- W4366088124 abstract "Desmoplasia is a highly aggressive histopathologic feature of cutaneous squamous cell carcinoma CSCC independently associated with death from CSCC. It is the deposition of abnormal collagen around a tumor as seen on H&E. However, the structure of this abnormal collagen is unknown. Herein, early immunofluorescence (IF) studies to define differences between normal and desmoplastic collagen structures are reported. Tumor samples from 27 CSCC patients were graded for desmoplasia using a semi-quantitative grading scale. Samples with prominent desmoplasia versus no desmoplasia were analyzed with bulk RNA-seq analysis by Nanostring technology. Additionally, the 27 CSCC and 7 normal skin samples were analyzed with the multispectral IF. K14 identified keratinocytes, and vimentin (VIM) identified fibroblasts. COL1A1 was a significantly upregulated gene in desmoplastic CSCC, as compared to non-desmoplasia (Log2FC=2, p<0.001). COL1 IF intensity was significantly decreased in desmoplastic stroma compared to non-desmoplasia (p<0.0001) and normal skin (p<0.0001). The IF staining pattern showed a markedly altered collagen I structure in desmoplastic stroma comprised of thin, wispy strands, in keeping with the H & E appearance of desmoplastic collagen. Surprisingly, COL1 intensity was significantly increased in tumor (keratinocyte) regions of desmoplastic CSCCs compared to tumor regions in non-desmoplastic CSCCs (p<0.0001) and normal epidermis (P<0.0001). Tumor degeneration was present in desmoplasia with COL1 staining in some tumor cells. This COL1-positive tumor phenotype was significantly increased in number cells/mm2compared to non-desmoplasia (413 vs 1101, p<0.0001). These findings indicate that collagen I expression in desmoplastic CSCC is quantifiably and structurally different from non-desmoplastic CSCC and native collagens. Desmoplastic tumor keratinocytes may produce the abnormal form of COL1 in desmoplastic CSCC." @default.
- W4366088124 created "2023-04-19" @default.
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- W4366088124 date "2023-05-01" @default.
- W4366088124 modified "2023-10-16" @default.
- W4366088124 title "294 Abnormal collagen I structure and protein expression in desmoplastic cutaneous squamous cell carcinoma" @default.
- W4366088124 doi "https://doi.org/10.1016/j.jid.2023.03.298" @default.
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