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- W4366091071 endingPage "598" @default.
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- W4366091071 abstract "Uveal melanoma (UM) is a rare malignant cancer of the eye, with up to 50% of patients dying from metastasis, for which no effective treatment is available. Due to the rarity of the disease, there is a great need to harness the limited material available from primary tumors and metastases for advanced research and preclinical drug screening. We established a platform to isolate, preserve, and transiently recover viable tissues, followed by the generation of spheroid cultures derived from primary UM. All assessed tumor-derived samples formed spheroids in culture within 24 h and stained positive for melanocyte-specific markers, indicating the retention of their melanocytic origin. These short-lived spheroids were only maintained for the duration of the experiment (7 days) or re-established from frozen tumor tissue acquired from the same patient. Intravenous injection of fluorescently labeled UM cells derived from these spheroids into zebrafish yielded a reproducible metastatic phenotype and recapitulated molecular features of the disseminating UM. This approach allowed for the experimental replications required for reliable drug screening (at least 2 individual biological experiments, with n > 20). Drug treatments with navitoclax and everolimus validated the zebrafish patient-derived model as a versatile preclinical tool for screening anti-UM drugs and as a preclinical platform to predict personalized drug responses." @default.
- W4366091071 created "2023-04-19" @default.
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- W4366091071 date "2023-04-15" @default.
- W4366091071 modified "2023-09-26" @default.
- W4366091071 title "Zebrafish Patient-Derived Xenograft Model as a Preclinical Platform for Uveal Melanoma Drug Discovery" @default.
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- W4366091071 cites W2024283519 @default.
- W4366091071 cites W2029345191 @default.
- W4366091071 cites W2038434969 @default.
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- W4366091071 cites W2139132031 @default.
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- W4366091071 cites W2190821820 @default.
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- W4366091071 cites W2331797622 @default.
- W4366091071 cites W2351450082 @default.
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- W4366091071 cites W2976161620 @default.
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- W4366091071 doi "https://doi.org/10.3390/ph16040598" @default.
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