FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366142405> ?p ?o ?g. }

• W4366142405 endingPage "8166" @default.
• W4366142405 startingPage "8153" @default.
• W4366142405 abstract "Blood-brain barrier (BBB) remains one of the critical challenges in developing neurological therapeutics. Short single-stranded DNA/RNA nucleotides forming a three-dimensional structure, called aptamers, have received increasing attention as BBB shuttles for efficient brain drug delivery owing to their practical advantages over Trojan horse antibodies or peptides. Aptamers are typically obtained by combinatorial chemical technology, termed Systemic Evolution of Ligands by EXponential Enrichment (SELEX), against purified targets, living cells, or animal models. However, identifying reliable BBB-penetrating aptamers that perform efficiently under human physiological conditions has been challenging because of the poor physiological relevance in the conventional SELEX process. Here, we report a human BBB shuttle aptamer (hBS) identified using a human microphysiological system (MPS)-based SELEX (MPS-SELEX) method. A two-channel MPS lined with human brain microvascular endothelial cells (BMECs) interfaced with astrocytes and pericytes, recapitulating high-level barrier function of in vivo BBB, was exploited as a screening platform. The MPS-SELEX procedure enabled robust function-based screening of the hBS candidates, which was not achievable in traditional in vitro BBB models. The identified aptamer (hBS01) through five-round of MPS-SELEX exhibited high capability to transport protein cargoes across the human BBB via clathrin-mediated endocytosis and enhanced uptake efficiency in BMECs and brain cells. The enhanced targeting specificity of hBS01 was further validated both in vitro and in vivo, confirming its powerful brain accumulation efficiency. These findings demonstrate that MPS-SELEX has potential in the discovery of aptamers with high target specificity that can be widely utilized to boost the development of drug delivery strategies." @default.
• W4366142405 created "2023-04-19" @default.
• W4366142405 creator A5004732089 @default.
• W4366142405 creator A5014715024 @default.
• W4366142405 creator A5018553594 @default.
• W4366142405 creator A5021191500 @default.
• W4366142405 creator A5040256311 @default.
• W4366142405 creator A5063847168 @default.
• W4366142405 creator A5065142278 @default.
• W4366142405 creator A5075338584 @default.
• W4366142405 creator A5077534985 @default.
• W4366142405 creator A5079746449 @default.
• W4366142405 creator A5090136775 @default.
• W4366142405 date "2023-04-17" @default.
• W4366142405 modified "2023-10-09" @default.
• W4366142405 title "Aptamer Nanoconstructs Crossing Human Blood–Brain Barrier Discovered via Microphysiological System-Based SELEX Technology" @default.
• W4366142405 cites W1964223712 @default.
• W4366142405 cites W1981328487 @default.
• W4366142405 cites W1995195889 @default.
• W4366142405 cites W2000164330 @default.
• W4366142405 cites W2009002151 @default.
• W4366142405 cites W2029440546 @default.
• W4366142405 cites W2035543500 @default.
• W4366142405 cites W2042883482 @default.
• W4366142405 cites W2048428129 @default.
• W4366142405 cites W2055129519 @default.
• W4366142405 cites W2056451525 @default.
• W4366142405 cites W2073262413 @default.
• W4366142405 cites W2074988418 @default.
• W4366142405 cites W2079321730 @default.
• W4366142405 cites W2086700602 @default.
• W4366142405 cites W2091497743 @default.
• W4366142405 cites W2092473496 @default.
• W4366142405 cites W2104525153 @default.
• W4366142405 cites W2125411036 @default.
• W4366142405 cites W2141157874 @default.
• W4366142405 cites W2156935100 @default.
• W4366142405 cites W2563103418 @default.
• W4366142405 cites W2567307082 @default.
• W4366142405 cites W2767330810 @default.
• W4366142405 cites W2793073482 @default.
• W4366142405 cites W2796081672 @default.
• W4366142405 cites W2910531243 @default.
• W4366142405 cites W2953383090 @default.
• W4366142405 cites W2972005444 @default.
• W4366142405 cites W2982242066 @default.
• W4366142405 cites W2989901716 @default.
• W4366142405 cites W3016537665 @default.
• W4366142405 cites W3026044843 @default.
• W4366142405 cites W3044994694 @default.
• W4366142405 cites W3135720699 @default.
• W4366142405 cites W3185034496 @default.
• W4366142405 cites W3203776961 @default.
• W4366142405 cites W4224222045 @default.
• W4366142405 cites W4230362313 @default.
• W4366142405 cites W4256220055 @default.
• W4366142405 doi "https://doi.org/10.1021/acsnano.2c11675" @default.
• W4366142405 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37068137" @default.
• W4366142405 hasPublicationYear "2023" @default.
• W4366142405 type Work @default.
• W4366142405 citedByCount "0" @default.
• W4366142405 crossrefType "journal-article" @default.
• W4366142405 hasAuthorship W4366142405A5004732089 @default.
• W4366142405 hasAuthorship W4366142405A5014715024 @default.
• W4366142405 hasAuthorship W4366142405A5018553594 @default.
• W4366142405 hasAuthorship W4366142405A5021191500 @default.
• W4366142405 hasAuthorship W4366142405A5040256311 @default.
• W4366142405 hasAuthorship W4366142405A5063847168 @default.
• W4366142405 hasAuthorship W4366142405A5065142278 @default.
• W4366142405 hasAuthorship W4366142405A5075338584 @default.
• W4366142405 hasAuthorship W4366142405A5077534985 @default.
• W4366142405 hasAuthorship W4366142405A5079746449 @default.
• W4366142405 hasAuthorship W4366142405A5090136775 @default.
• W4366142405 hasConcept C104317684 @default.
• W4366142405 hasConcept C122682173 @default.
• W4366142405 hasConcept C128972844 @default.
• W4366142405 hasConcept C1491633281 @default.
• W4366142405 hasConcept C153911025 @default.
• W4366142405 hasConcept C28005876 @default.
• W4366142405 hasConcept C55493867 @default.
• W4366142405 hasConcept C67705224 @default.
• W4366142405 hasConcept C70721500 @default.
• W4366142405 hasConcept C86803240 @default.
• W4366142405 hasConceptScore W4366142405C104317684 @default.
• W4366142405 hasConceptScore W4366142405C122682173 @default.
• W4366142405 hasConceptScore W4366142405C128972844 @default.
• W4366142405 hasConceptScore W4366142405C1491633281 @default.
• W4366142405 hasConceptScore W4366142405C153911025 @default.
• W4366142405 hasConceptScore W4366142405C28005876 @default.
• W4366142405 hasConceptScore W4366142405C55493867 @default.
• W4366142405 hasConceptScore W4366142405C67705224 @default.
• W4366142405 hasConceptScore W4366142405C70721500 @default.
• W4366142405 hasConceptScore W4366142405C86803240 @default.
• W4366142405 hasFunder F4320321348 @default.
• W4366142405 hasFunder F4320322034 @default.
• W4366142405 hasFunder F4320322120 @default.
• W4366142405 hasIssue "9" @default.
• W4366142405 hasLocation W43661424051 @default.

• next