Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366171864> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W4366171864 endingPage "S144" @default.
- W4366171864 startingPage "S144" @default.
- W4366171864 abstract "Recent studies have revealed the importance of IL-15 signaling in the pathogenesis of vitiligo. Although no SNPs located in genes related to IL-15 signaling have been identified by GWAS studies for vitiligo, a 3D genome analysis revealed that a GWAS-identified SNP (rs706779), located in an intronic region of IL2RA and 70 kb away from IL15RA, could interact with IL15RA in T cells. Since epidermal keratinocytes upregulate expression of IL15RA in vitiligo, we investigated the impact of the SNP in keratinocytes. First, we identified that the region of the SNP supports the expression of IL15RA in keratinocytes by using CRISPR/Cas9-based genome editing. Second, we found the most common functional isoform of IL15RA is more highly expressed in keratinocytes with homozygous risk alleles of rs706779 (T/T) compared to those with homozygous protective alleles (C/C) for vitiligo. Surprisingly, electrophoretic mobility shift assay and chromatin immunoprecipitation assay showed that the risk allele had weaker binding ability of NF-κB p65, which resulted in less recruitment of DDX17, a modifier of RNA processing including pre-mRNA splicing. Further, the knockdown of DDX17 in keratinocytes increased the expression ratio of functional isoforms to a non-functional one that lacks exon 2, responsible for binding IL-15 (Δ2 isoform). In addition, knockdown of the Δ2 isoform of IL15RA in keratinocytes upregulated the functional isoform, and enhanced the activation of the memory phenotype of CD8+T cells. Together, our data support a unique functional role of the rs706779 SNP in keratinocytes, to promote T cell activation by regulating the isoform profile of IL15RA." @default.
- W4366171864 created "2023-04-19" @default.
- W4366171864 creator A5002640734 @default.
- W4366171864 creator A5010001634 @default.
- W4366171864 creator A5014979110 @default.
- W4366171864 creator A5015491256 @default.
- W4366171864 creator A5035000472 @default.
- W4366171864 creator A5043553227 @default.
- W4366171864 creator A5046400781 @default.
- W4366171864 creator A5050388243 @default.
- W4366171864 creator A5053317702 @default.
- W4366171864 creator A5055674018 @default.
- W4366171864 creator A5076574608 @default.
- W4366171864 creator A5088270417 @default.
- W4366171864 date "2023-05-01" @default.
- W4366171864 modified "2023-10-03" @default.
- W4366171864 title "841 A vitiligo-associated SNP (rs706779) controls IL15RA isoforms and T cell activation in epidermal keratinocytes" @default.
- W4366171864 doi "https://doi.org/10.1016/j.jid.2023.03.851" @default.
- W4366171864 hasPublicationYear "2023" @default.
- W4366171864 type Work @default.
- W4366171864 citedByCount "0" @default.
- W4366171864 crossrefType "journal-article" @default.
- W4366171864 hasAuthorship W4366171864A5002640734 @default.
- W4366171864 hasAuthorship W4366171864A5010001634 @default.
- W4366171864 hasAuthorship W4366171864A5014979110 @default.
- W4366171864 hasAuthorship W4366171864A5015491256 @default.
- W4366171864 hasAuthorship W4366171864A5035000472 @default.
- W4366171864 hasAuthorship W4366171864A5043553227 @default.
- W4366171864 hasAuthorship W4366171864A5046400781 @default.
- W4366171864 hasAuthorship W4366171864A5050388243 @default.
- W4366171864 hasAuthorship W4366171864A5053317702 @default.
- W4366171864 hasAuthorship W4366171864A5055674018 @default.
- W4366171864 hasAuthorship W4366171864A5076574608 @default.
- W4366171864 hasAuthorship W4366171864A5088270417 @default.
- W4366171864 hasBestOaLocation W43661718641 @default.
- W4366171864 hasConcept C104317684 @default.
- W4366171864 hasConcept C106208931 @default.
- W4366171864 hasConcept C127561419 @default.
- W4366171864 hasConcept C135763542 @default.
- W4366171864 hasConcept C139275648 @default.
- W4366171864 hasConcept C153209595 @default.
- W4366171864 hasConcept C153911025 @default.
- W4366171864 hasConcept C173396325 @default.
- W4366171864 hasConcept C180754005 @default.
- W4366171864 hasConcept C2777074287 @default.
- W4366171864 hasConcept C2779980618 @default.
- W4366171864 hasConcept C36823959 @default.
- W4366171864 hasConcept C502942594 @default.
- W4366171864 hasConcept C53345823 @default.
- W4366171864 hasConcept C54355233 @default.
- W4366171864 hasConcept C81885089 @default.
- W4366171864 hasConcept C86803240 @default.
- W4366171864 hasConceptScore W4366171864C104317684 @default.
- W4366171864 hasConceptScore W4366171864C106208931 @default.
- W4366171864 hasConceptScore W4366171864C127561419 @default.
- W4366171864 hasConceptScore W4366171864C135763542 @default.
- W4366171864 hasConceptScore W4366171864C139275648 @default.
- W4366171864 hasConceptScore W4366171864C153209595 @default.
- W4366171864 hasConceptScore W4366171864C153911025 @default.
- W4366171864 hasConceptScore W4366171864C173396325 @default.
- W4366171864 hasConceptScore W4366171864C180754005 @default.
- W4366171864 hasConceptScore W4366171864C2777074287 @default.
- W4366171864 hasConceptScore W4366171864C2779980618 @default.
- W4366171864 hasConceptScore W4366171864C36823959 @default.
- W4366171864 hasConceptScore W4366171864C502942594 @default.
- W4366171864 hasConceptScore W4366171864C53345823 @default.
- W4366171864 hasConceptScore W4366171864C54355233 @default.
- W4366171864 hasConceptScore W4366171864C81885089 @default.
- W4366171864 hasConceptScore W4366171864C86803240 @default.
- W4366171864 hasIssue "5" @default.
- W4366171864 hasLocation W43661718641 @default.
- W4366171864 hasOpenAccess W4366171864 @default.
- W4366171864 hasPrimaryLocation W43661718641 @default.
- W4366171864 hasRelatedWork W1935916792 @default.
- W4366171864 hasRelatedWork W2057755081 @default.
- W4366171864 hasRelatedWork W2089212629 @default.
- W4366171864 hasRelatedWork W2161808406 @default.
- W4366171864 hasRelatedWork W2163968639 @default.
- W4366171864 hasRelatedWork W2347229162 @default.
- W4366171864 hasRelatedWork W2363553001 @default.
- W4366171864 hasRelatedWork W2365367142 @default.
- W4366171864 hasRelatedWork W2982228282 @default.
- W4366171864 hasRelatedWork W3113918231 @default.
- W4366171864 hasVolume "143" @default.
- W4366171864 isParatext "false" @default.
- W4366171864 isRetracted "false" @default.
- W4366171864 workType "article" @default.