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- W4366185790 abstract "Tyrosinase catalyzes the initial, rate-limiting step of the melanin synthesis; oxidative conversion of L-tyrosine to L-dopa followed by L-dopaquinone by means of oxygen molecule. Tyrosinase possesses bicopper active center capable of utilizing molecular oxygen. Metabolic conversion of rhododendrol (RD) by tyrosinase causes leukoderma in a certain population of consumers. Along with cytotoxic RD metabolites, reactive oxygen species (ROS) produced during the RD metabolism appears to cause melanocyte death. However, actual source for ROS is still elusive. Some phenolic compounds are known to act as suicide substrates for tyrosinase, and, during the catalytic reaction, one of two copper ions along with hydrogen peroxide is released from tyrosinase. We hypothesized that RD might be a potent suicide substrate for tyrosinase and that the released copper ion is responsible for melanocyte death. Incubation of human melanocytes with RD showed a decrease in tyrosinase activity. Among the three strains of melanocytes, the strain with the highest tyrosinase activity was the most susceptible to RD-induced cell death. Inhibitors for apoptosis or ferroptosis did not significantly alleviate the RD-induced melanocyte death. Both the tyrosinase inhibitor phenylthiourea (PTU) and the copper chelator D-penicillamine (DPA) significantly alleviated the RD-induced melanocyte death. PTU irreversibly inhibited tyrosinase, whereas DPA had no effects on the enzymatic activity. The protective effect of DPA appears to be due to its ability to chelate copper ion rather than direct inhibition of tyrosinase. RD appears to act as the suicide substrate and result in copper ion release along with production of hydrogen peroxides, which collectively cause oxidative damages on melanocyte. These observations suggest that copper chelation may become a general prevention for chemical leukoderma caused by aberrant tyrosinase activity." @default.
- W4366185790 created "2023-04-19" @default.
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- W4366185790 date "2023-05-01" @default.
- W4366185790 modified "2023-09-28" @default.
- W4366185790 title "1242 Suicide substrate properties of rhododendrol are responsible for tyrosinase inactivation and melanocyte death" @default.
- W4366185790 doi "https://doi.org/10.1016/j.jid.2023.03.1256" @default.
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