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- W4366185807 abstract "Extracorporeal photopheresis (ECP), a therapy using extracorporeal blood circulation and ultraviolet light, is effective for cutaneous T-cell lymphoma, etc. The mechanism of action of ECP is T cell apoptosis and induction of T regulatory cells. In conventional ECP, leukocytes are separated from peripheral blood and 8-methoxypsoralen is added as a photosensitizer. After UVA irradiation, the leukocytes are returned to the patient's circulating blood. The size and complexity of the device and the need for shading after treatment, however, are problematic. Therefore, we aimed to develop a new technology for ECP that does not use centrifugation or a photosensitizer. First, the optimal inner diameter of the quartz tube was determined using peripheral blood and quartz tubes with different inner diameters ranging from 200μm to 900μm. The smaller the inner diameter, the more apoptosis was induced, but the optimal inner diameter was 300μm because 200μm would cause problems such as coagulation. Next, Jurkat cells labeled by a cell proliferation assay were mixed with peripheral blood. The mixed Jurkat cells were passed through a flow system composed of a quartz capillary tube and a peristaltic pump, and irradiated with an LED (λ: 290 nm) as they passed through the quartz capillary tube. Irradiated Jurkat cells were cultured for 24 h after adding a medium, and apoptosis was measured by fluorescein-activation cell sorting. Peripheral blood was also irradiated in the same system and live / dead cells were measured by fluorescein-activation cell sorting. Our findings confirmed that apoptosis can be induced even in a flow irradiation system, and the apoptosis rate increases depending on the irradiation dose. We further confirmed that CD4 in the irradiated peripheral blood induced cell death. These results suggest that apoptosis of targeted cells can be induced even in systems that do not use centrifugation or photosensitizers." @default.
- W4366185807 created "2023-04-19" @default.
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- W4366185807 date "2023-05-01" @default.
- W4366185807 modified "2023-09-28" @default.
- W4366185807 title "1149 Extracorporeal photopheresis with quartz capillary tubes and UVC light emitting diodes does not require photosensitizers and centrifugation" @default.
- W4366185807 doi "https://doi.org/10.1016/j.jid.2023.03.1162" @default.
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