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- W4366191123 abstract "The human microbiome has a fundamental role in skin homeostasis, barrier function and host immune networks. Acute and chronic skin conditions are often associated with imbalances in host-microbiome-immune interactions. Despite recent advances in skin microbiome research, there are limited models for studying these interactions in healthy and diseased skin, therefore we have developed a model to investigate how different microbiome profiles alter epidermal homeostasis and T-cell responses. Full-thickness skin models were colonized with five human microbiome species:Staphylococcus epidermidis (SE), Staphylococcus capitis (SC), Cutibacterium acnes (CA), Malassezia restricta (MR) and Malassezia globosa (MG), in singular or combined (5M) inoculations.The effect of colonization on epidermal differentiation and barrier formation was observed via the expression of K14, K10, E-cadherin and Ki67. Increased expression of K14 was observed in models colonized with SE, SC (p=0.0062) and 5M. MG colonization significantly reduced K10 expression (p=0.0180). Expression of E-cadherin significantly decreased in models colonised with MR (p=0.0489). Despite significant changes in epidermal thickness, Ki67 expression was not altered across conditions. Commensal microbes have been shown to regulate epidermal barrier function via the aryl hydrocarbon receptor(AhR). Increased nuclear expression of AhR was observed in response to SC, CA and 5M, suggesting a greater role in AhR activation. Model colonization influenced the expression of CD69, cutaneous lymphocyte antigen (CLA) and HLA-DR on T cells in response to incubating PBMCs with conditioned media from inoculated models. SE caused a significant increase in CD3+/CLA+ cells (p=0.0362) and 5M significantly reduced CD3+/HLA-DR+ cells (p=0.0304). RNA-sequencing aims to support current analysis and elucidate any more targets to be explored. Our findings suggest different microbial profiles alter epidermal barrier formation and immune responses." @default.
- W4366191123 created "2023-04-19" @default.
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- W4366191123 date "2023-05-01" @default.
- W4366191123 modified "2023-09-28" @default.
- W4366191123 title "910 Exploring host-microbiome interactions in optimised 3D skin models" @default.
- W4366191123 doi "https://doi.org/10.1016/j.jid.2023.03.921" @default.
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