Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366234881> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W4366234881 abstract "Fluorescent proteins (FPs) have become an essential tool for biological research. Since the isolation and description of green FP, hundreds of FPs have been discovered and created with various characteristics. The excitation of these proteins ranges from ultraviolet (UV) up to near infrared (NIR). Using conventional cytometry, with each detector assigned to a fluorochrome, great care must be taken when selecting the optimal bandpass filters to minimalize the spectral overlap as the emission spectra of FPs are broad. Full-spectrum flow cytometers eliminate the need to change optical filters for analyzing FPs, which simplifies instrument setup. In experiments where more than one FP is used, single-color controls are required. These can be cells expressing each of the proteins separately. In the case of the confetti system, for instance, when four FPs are used, all these proteins will need to be expressed separately so that compensation or spectral unmixing can be performed, and this can be inconvenient and expensive. An appealing alternative is to produce FPs in Escherichia coli, purify them, and covalently couple them to carboxylate polystyrene microspheres. Such microspheres are ready to use and can be stored at 4°C for months or even years without any deterioration in fluorescence. The same procedure can be used to couple antibodies or other proteins to these particles. Here, we describe how to express and purify FPs, how to couple them to microspheres, and how to evaluate the fluorescent properties of the particles. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Escherichia coli expression and purification of recombinant mPlum Basic Protocol 2: Coupling fluorescent proteins to polystyrene microspheres Support Protocol 1: Comparing the cell-bound and bead-bound fluorescence signatures Support Protocol 2: Comparing spectral signatures via the similarity index, complexity matrix, and spillover spread matrix of fluorescent protein-coupled beads." @default.
- W4366234881 created "2023-04-20" @default.
- W4366234881 creator A5034159414 @default.
- W4366234881 creator A5079055272 @default.
- W4366234881 creator A5086802226 @default.
- W4366234881 date "2023-04-01" @default.
- W4366234881 modified "2023-09-26" @default.
- W4366234881 title "Fluorescent Protein Production, Purification, and Coupling to Microspheres" @default.
- W4366234881 cites W2007981920 @default.
- W4366234881 cites W2032579724 @default.
- W4366234881 cites W2055686479 @default.
- W4366234881 cites W2058211348 @default.
- W4366234881 cites W2084312697 @default.
- W4366234881 cites W2087537126 @default.
- W4366234881 cites W2139414608 @default.
- W4366234881 cites W2140300773 @default.
- W4366234881 cites W2548873975 @default.
- W4366234881 cites W4223478145 @default.
- W4366234881 doi "https://doi.org/10.1002/cpz1.745" @default.
- W4366234881 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37071053" @default.
- W4366234881 hasPublicationYear "2023" @default.
- W4366234881 type Work @default.
- W4366234881 citedByCount "0" @default.
- W4366234881 crossrefType "journal-article" @default.
- W4366234881 hasAuthorship W4366234881A5034159414 @default.
- W4366234881 hasAuthorship W4366234881A5079055272 @default.
- W4366234881 hasAuthorship W4366234881A5086802226 @default.
- W4366234881 hasBestOaLocation W43662348811 @default.
- W4366234881 hasConcept C104317684 @default.
- W4366234881 hasConcept C120665830 @default.
- W4366234881 hasConcept C121332964 @default.
- W4366234881 hasConcept C127413603 @default.
- W4366234881 hasConcept C142613039 @default.
- W4366234881 hasConcept C159985019 @default.
- W4366234881 hasConcept C171250308 @default.
- W4366234881 hasConcept C185592680 @default.
- W4366234881 hasConcept C186060115 @default.
- W4366234881 hasConcept C189942447 @default.
- W4366234881 hasConcept C192562407 @default.
- W4366234881 hasConcept C2776798109 @default.
- W4366234881 hasConcept C2777922577 @default.
- W4366234881 hasConcept C3019336641 @default.
- W4366234881 hasConcept C41008148 @default.
- W4366234881 hasConcept C42360764 @default.
- W4366234881 hasConcept C49040817 @default.
- W4366234881 hasConcept C521977710 @default.
- W4366234881 hasConcept C547475151 @default.
- W4366234881 hasConcept C55493867 @default.
- W4366234881 hasConcept C86803240 @default.
- W4366234881 hasConcept C91881484 @default.
- W4366234881 hasConceptScore W4366234881C104317684 @default.
- W4366234881 hasConceptScore W4366234881C120665830 @default.
- W4366234881 hasConceptScore W4366234881C121332964 @default.
- W4366234881 hasConceptScore W4366234881C127413603 @default.
- W4366234881 hasConceptScore W4366234881C142613039 @default.
- W4366234881 hasConceptScore W4366234881C159985019 @default.
- W4366234881 hasConceptScore W4366234881C171250308 @default.
- W4366234881 hasConceptScore W4366234881C185592680 @default.
- W4366234881 hasConceptScore W4366234881C186060115 @default.
- W4366234881 hasConceptScore W4366234881C189942447 @default.
- W4366234881 hasConceptScore W4366234881C192562407 @default.
- W4366234881 hasConceptScore W4366234881C2776798109 @default.
- W4366234881 hasConceptScore W4366234881C2777922577 @default.
- W4366234881 hasConceptScore W4366234881C3019336641 @default.
- W4366234881 hasConceptScore W4366234881C41008148 @default.
- W4366234881 hasConceptScore W4366234881C42360764 @default.
- W4366234881 hasConceptScore W4366234881C49040817 @default.
- W4366234881 hasConceptScore W4366234881C521977710 @default.
- W4366234881 hasConceptScore W4366234881C547475151 @default.
- W4366234881 hasConceptScore W4366234881C55493867 @default.
- W4366234881 hasConceptScore W4366234881C86803240 @default.
- W4366234881 hasConceptScore W4366234881C91881484 @default.
- W4366234881 hasIssue "4" @default.
- W4366234881 hasLocation W43662348811 @default.
- W4366234881 hasLocation W43662348812 @default.
- W4366234881 hasOpenAccess W4366234881 @default.
- W4366234881 hasPrimaryLocation W43662348811 @default.
- W4366234881 hasRelatedWork W1796081043 @default.
- W4366234881 hasRelatedWork W2072868568 @default.
- W4366234881 hasRelatedWork W2356763595 @default.
- W4366234881 hasRelatedWork W2533770197 @default.
- W4366234881 hasRelatedWork W2619614270 @default.
- W4366234881 hasRelatedWork W2748952813 @default.
- W4366234881 hasRelatedWork W2899084033 @default.
- W4366234881 hasRelatedWork W2969104560 @default.
- W4366234881 hasRelatedWork W2981745317 @default.
- W4366234881 hasRelatedWork W3167440593 @default.
- W4366234881 hasVolume "3" @default.
- W4366234881 isParatext "false" @default.
- W4366234881 isRetracted "false" @default.
- W4366234881 workType "article" @default.