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• W4366259796 startingPage "6828" @default.
• W4366259796 abstract "Aptamers have been employed as the biorecognition element in electrochemical aptamer-based (E-AB) biosensors, for the detection of a diverse range of analyte molecules, on electrodes with sizescales ranging from a few microns to several millimeters. Simultaneous detection of multiple different analytes requires the selective modification of multiple electrode surfaces with different aptamers. This process is typically achieved by incubating separate macroscale electrodes in a solution with the desired aptamer, which is unsuitable for microelectrode arrays in which the electrodes are closely spaced. In this work, we selectively modified electrode surfaces with thiolated aptamers of different single-stranded DNA sequences, by successive removal and addition of thiol monolayers. This was achieved by electrodesorption of thiol monolayers using controlled potential, to expose unmodified gold electrodes to be modified with a different thiolated aptamer, thus enabling multiple different aptamers to be used on the surfaces of closely spaced microelectrodes. All aptamers were methylene blue terminated, allowing redox currents to be measured and used to monitor aptamer probe packing density on the electrode surface and the selectivity of the sensors. Here, we demonstrate the microscale E-AB sensor multianalyte detection method using aptamers for target analytes, adenosine triphosphate, dopamine, and serotonin, which can ultimately be applied to perform localized simultaneous detection using electrode arrays." @default.
• W4366259796 created "2023-04-20" @default.
• W4366259796 creator A5027432595 @default.
• W4366259796 creator A5035638066 @default.
• W4366259796 date "2023-04-18" @default.
• W4366259796 modified "2023-10-16" @default.
• W4366259796 title "Selective Aptamer Modification of Au Surfaces in a Microelectrode Sensor Array for Simultaneous Detection of Multiple Analytes" @default.
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• W4366259796 doi "https://doi.org/10.1021/acs.analchem.2c05335" @default.
• W4366259796 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37071798" @default.
• W4366259796 hasPublicationYear "2023" @default.
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