FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366334423> ?p ?o ?g. }

• W4366334423 abstract "Abstract Background Argonaute proteins play a central role in RNA silencing by forming protein-small RNA complexes responsible for the silencing process. While most Argonaute proteins have a short N-terminal region, Argonaute2 in Drosophila melanogaster (DmAgo2) harbors a long and unique N-terminal region. Previous in vitro biochemical studies have shown that the loss of this region does not impair the RNA silencing activity of the complex. However, an N-terminal mutant of Drosophila melanogaster has demonstrated abnormal RNA silencing activity. To explore the causes of this discrepancy between in vitro and in vivo studies, we investigated the biophysical properties of the region. The N-terminal region is highly rich in glutamine and glycine residues, which is a well-known property for prion-like domains, a subclass of amyloid-forming peptides. Therefore, the possibility of the N-terminal region functioning as an amyloid was tested. Results Our in silico and biochemical assays demonstrated that the N-terminal region exhibits amyloid-specific properties. The region indeed formed aggregates that were not dissociated even in the presence of sodium dodecyl sulfate. Also, the aggregates enhanced the fluorescence intensity of thioflavin-T, an amyloid detection reagent. The kinetics of the aggregation followed that of typical amyloid formation exhibiting self-propagating activity. Furthermore, we directly visualized the aggregation process of the N-terminal region under fluorescence microscopy and found that the aggregations took fractal or fibril shapes. Together, the results indicate that the N-terminal region can form amyloid-like aggregates. Conclusions Many other amyloid-forming peptides have been reported to modulate the function of proteins through their aggregation. Therefore, our findings raise the possibility that aggregation of the N-terminal region regulates the RNA silencing activity of DmAgo2." @default.
• W4366334423 created "2023-04-20" @default.
• W4366334423 creator A5019735246 @default.
• W4366334423 creator A5045957255 @default.
• W4366334423 creator A5053143143 @default.
• W4366334423 creator A5068110977 @default.
• W4366334423 date "2023-04-19" @default.
• W4366334423 modified "2023-10-15" @default.
• W4366334423 title "N-terminal region of Drosophila melanogaster Argonaute2 forms amyloid-like aggregates" @default.
• W4366334423 cites W1601640901 @default.
• W4366334423 cites W1917144187 @default.
• W4366334423 cites W1966185641 @default.
• W4366334423 cites W1970679679 @default.
• W4366334423 cites W1971674915 @default.
• W4366334423 cites W1977034658 @default.
• W4366334423 cites W1981155219 @default.
• W4366334423 cites W1987154973 @default.
• W4366334423 cites W1992508643 @default.
• W4366334423 cites W2011301426 @default.
• W4366334423 cites W2015159529 @default.
• W4366334423 cites W2028648109 @default.
• W4366334423 cites W2049392988 @default.
• W4366334423 cites W2049476357 @default.
• W4366334423 cites W2053820259 @default.
• W4366334423 cites W2055755496 @default.
• W4366334423 cites W2060303574 @default.
• W4366334423 cites W2067644428 @default.
• W4366334423 cites W2074106269 @default.
• W4366334423 cites W2077790507 @default.
• W4366334423 cites W2080356693 @default.
• W4366334423 cites W2115734610 @default.
• W4366334423 cites W2119651404 @default.
• W4366334423 cites W2121753059 @default.
• W4366334423 cites W2124259497 @default.
• W4366334423 cites W2129602427 @default.
• W4366334423 cites W2132121416 @default.
• W4366334423 cites W2136813339 @default.
• W4366334423 cites W2137450588 @default.
• W4366334423 cites W2137753785 @default.
• W4366334423 cites W2139995722 @default.
• W4366334423 cites W2149077867 @default.
• W4366334423 cites W2149732267 @default.
• W4366334423 cites W2151474826 @default.
• W4366334423 cites W2154824097 @default.
• W4366334423 cites W2165119400 @default.
• W4366334423 cites W2396676392 @default.
• W4366334423 cites W2590568317 @default.
• W4366334423 cites W2606007784 @default.
• W4366334423 cites W2760587614 @default.
• W4366334423 cites W2775487530 @default.
• W4366334423 cites W2802935265 @default.
• W4366334423 cites W2803819558 @default.
• W4366334423 cites W2890980244 @default.
• W4366334423 cites W2950858826 @default.
• W4366334423 cites W2951683236 @default.
• W4366334423 cites W3006870410 @default.
• W4366334423 cites W3013114619 @default.
• W4366334423 cites W3015539733 @default.
• W4366334423 cites W3103145119 @default.
• W4366334423 cites W3164707132 @default.
• W4366334423 cites W3211795435 @default.
• W4366334423 cites W4205867548 @default.
• W4366334423 doi "https://doi.org/10.1186/s12915-023-01569-3" @default.
• W4366334423 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37072852" @default.
• W4366334423 hasPublicationYear "2023" @default.
• W4366334423 type Work @default.
• W4366334423 citedByCount "0" @default.
• W4366334423 crossrefType "journal-article" @default.
• W4366334423 hasAuthorship W4366334423A5019735246 @default.
• W4366334423 hasAuthorship W4366334423A5045957255 @default.
• W4366334423 hasAuthorship W4366334423A5053143143 @default.
• W4366334423 hasAuthorship W4366334423A5068110977 @default.
• W4366334423 hasBestOaLocation W43663344231 @default.
• W4366334423 hasConcept C104317684 @default.
• W4366334423 hasConcept C119056186 @default.
• W4366334423 hasConcept C12554922 @default.
• W4366334423 hasConcept C142724271 @default.
• W4366334423 hasConcept C153911025 @default.
• W4366334423 hasConcept C166703698 @default.
• W4366334423 hasConcept C201853390 @default.
• W4366334423 hasConcept C2777633098 @default.
• W4366334423 hasConcept C2778896982 @default.
• W4366334423 hasConcept C2779134260 @default.
• W4366334423 hasConcept C2780104201 @default.
• W4366334423 hasConcept C502032728 @default.
• W4366334423 hasConcept C55493867 @default.
• W4366334423 hasConcept C59822182 @default.
• W4366334423 hasConcept C67705224 @default.
• W4366334423 hasConcept C71924100 @default.
• W4366334423 hasConcept C86803240 @default.
• W4366334423 hasConcept C95444343 @default.
• W4366334423 hasConceptScore W4366334423C104317684 @default.
• W4366334423 hasConceptScore W4366334423C119056186 @default.
• W4366334423 hasConceptScore W4366334423C12554922 @default.
• W4366334423 hasConceptScore W4366334423C142724271 @default.
• W4366334423 hasConceptScore W4366334423C153911025 @default.
• W4366334423 hasConceptScore W4366334423C166703698 @default.
• W4366334423 hasConceptScore W4366334423C201853390 @default.
• W4366334423 hasConceptScore W4366334423C2777633098 @default.
• W4366334423 hasConceptScore W4366334423C2778896982 @default.

• next