FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366338975> ?p ?o ?g. }

• W4366338975 abstract "Abstract Background Sorafenib is a first-line chemotherapy drug for treating advanced hepatocellular carcinoma (HCC). However, its therapeutic effect has been seriously affected by the emergence of sorafenib resistance in HCC patients. The underlying mechanism of sorafenib resistance is unclear. Here, we report a circular RNA, cDCBLD2, which plays an important role in sorafenib resistance in HCC. Methods Use in vitro and in vivo experiments to study the function of cDCBLD2. Immunohistochemical detection of type IIA topoisomerase (TOP2A) expression in HCC tissue. The downstream molecules of cDCBLD2 were identified by differential gene analysis after knocking down cDCBLD2. QPCR, pull-down assay, FISH, Western blotting, dual-Luciferase reporter, cck8 assay, flow cytometry and patient-derived xenograft (PDX) mice were used to determine the molecular mechanism. Results We found that cDCBLD2 was upregulated in sorafenib-resistant HCC cells, and knocking down cDCBLD2 expression could significantly increase sorafenib-related cytotoxicity. Further evidence showed that cDCBLD2 can bind to microRNA (miR)-345-5p through a competing endogenous RNA mechanism, increase type TOP2A mRNA stability through a miRNA sponge mechanism, and reduce the effects of sorafenib treatment on HCC by inhibiting apoptosis. Our findings also suggest that miR-345-5p can negatively regulate TOP2A levels by binding to the coding sequence region of its mRNA. Additionally, targeting cDCBLD2 by injecting a specific small interfering RNA (siRNA) could significantly overcome sorafenib resistance in a PDX mouse model of liver cancer. Conclusion We found that cDCBLD2 can competitively bind to the TOP2A coding sequence region through the miR-345-5p sponge mechanism, and thus affect sorafenib resistance and caspase-3 mediated apoptosis of HCC cells. Taken together, our study provides a proof-of-concept for a potential strategy to overcome sorafenib resistance in HCC patients by targeting cDCBLD2 or TOP2A." @default.
• W4366338975 created "2023-04-20" @default.
• W4366338975 creator A5012152127 @default.
• W4366338975 creator A5020830182 @default.
• W4366338975 creator A5045801071 @default.
• W4366338975 creator A5053051398 @default.
• W4366338975 creator A5073199916 @default.
• W4366338975 creator A5074676756 @default.
• W4366338975 creator A5077783491 @default.
• W4366338975 creator A5086510500 @default.
• W4366338975 creator A5090911302 @default.
• W4366338975 creator A5091069161 @default.
• W4366338975 date "2023-04-18" @default.
• W4366338975 modified "2023-10-18" @default.
• W4366338975 title "cDCBLD2 mediates sorafenib resistance in hepatocellular carcinoma by sponging miR-345-5p binding to the TOP2A coding sequence" @default.
• W4366338975 cites W1593934359 @default.
• W4366338975 cites W1821077834 @default.
• W4366338975 cites W2022370669 @default.
• W4366338975 cites W2043510752 @default.
• W4366338975 cites W2046314136 @default.
• W4366338975 cites W2053490054 @default.
• W4366338975 cites W2064165694 @default.
• W4366338975 cites W2157916750 @default.
• W4366338975 cites W2158025958 @default.
• W4366338975 cites W2166550586 @default.
• W4366338975 cites W2568292531 @default.
• W4366338975 cites W2592988313 @default.
• W4366338975 cites W2600519390 @default.
• W4366338975 cites W2751143162 @default.
• W4366338975 cites W2982161529 @default.
• W4366338975 cites W2983138788 @default.
• W4366338975 cites W2999483628 @default.
• W4366338975 cites W3016976918 @default.
• W4366338975 cites W3025921786 @default.
• W4366338975 cites W3037315173 @default.
• W4366338975 cites W3044534510 @default.
• W4366338975 cites W3095202855 @default.
• W4366338975 cites W3100629716 @default.
• W4366338975 cites W3109268452 @default.
• W4366338975 cites W3117640930 @default.
• W4366338975 cites W3128646645 @default.
• W4366338975 cites W3134776089 @default.
• W4366338975 cites W3163118460 @default.
• W4366338975 cites W4220739183 @default.
• W4366338975 doi "https://doi.org/10.21203/rs.3.rs-2795751/v1" @default.
• W4366338975 hasPublicationYear "2023" @default.
• W4366338975 type Work @default.
• W4366338975 citedByCount "0" @default.
• W4366338975 crossrefType "posted-content" @default.
• W4366338975 hasAuthorship W4366338975A5012152127 @default.
• W4366338975 hasAuthorship W4366338975A5020830182 @default.
• W4366338975 hasAuthorship W4366338975A5045801071 @default.
• W4366338975 hasAuthorship W4366338975A5053051398 @default.
• W4366338975 hasAuthorship W4366338975A5073199916 @default.
• W4366338975 hasAuthorship W4366338975A5074676756 @default.
• W4366338975 hasAuthorship W4366338975A5077783491 @default.
• W4366338975 hasAuthorship W4366338975A5086510500 @default.
• W4366338975 hasAuthorship W4366338975A5090911302 @default.
• W4366338975 hasAuthorship W4366338975A5091069161 @default.
• W4366338975 hasBestOaLocation W43663389751 @default.
• W4366338975 hasConcept C104317684 @default.
• W4366338975 hasConcept C127561419 @default.
• W4366338975 hasConcept C145059251 @default.
• W4366338975 hasConcept C150903083 @default.
• W4366338975 hasConcept C153911025 @default.
• W4366338975 hasConcept C185592680 @default.
• W4366338975 hasConcept C207001950 @default.
• W4366338975 hasConcept C22615655 @default.
• W4366338975 hasConcept C2778019345 @default.
• W4366338975 hasConcept C2778695046 @default.
• W4366338975 hasConcept C502942594 @default.
• W4366338975 hasConcept C55493867 @default.
• W4366338975 hasConcept C60365752 @default.
• W4366338975 hasConcept C62203573 @default.
• W4366338975 hasConcept C67705224 @default.
• W4366338975 hasConcept C86803240 @default.
• W4366338975 hasConceptScore W4366338975C104317684 @default.
• W4366338975 hasConceptScore W4366338975C127561419 @default.
• W4366338975 hasConceptScore W4366338975C145059251 @default.
• W4366338975 hasConceptScore W4366338975C150903083 @default.
• W4366338975 hasConceptScore W4366338975C153911025 @default.
• W4366338975 hasConceptScore W4366338975C185592680 @default.
• W4366338975 hasConceptScore W4366338975C207001950 @default.
• W4366338975 hasConceptScore W4366338975C22615655 @default.
• W4366338975 hasConceptScore W4366338975C2778019345 @default.
• W4366338975 hasConceptScore W4366338975C2778695046 @default.
• W4366338975 hasConceptScore W4366338975C502942594 @default.
• W4366338975 hasConceptScore W4366338975C55493867 @default.
• W4366338975 hasConceptScore W4366338975C60365752 @default.
• W4366338975 hasConceptScore W4366338975C62203573 @default.
• W4366338975 hasConceptScore W4366338975C67705224 @default.
• W4366338975 hasConceptScore W4366338975C86803240 @default.
• W4366338975 hasLocation W43663389751 @default.
• W4366338975 hasOpenAccess W4366338975 @default.
• W4366338975 hasPrimaryLocation W43663389751 @default.
• W4366338975 hasRelatedWork W2094049211 @default.
• W4366338975 hasRelatedWork W2392866045 @default.
• W4366338975 hasRelatedWork W2888296588 @default.
• W4366338975 hasRelatedWork W2981291069 @default.
• W4366338975 hasRelatedWork W3011149411 @default.

• next