FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366339110> ?p ?o ?g. }

• W4366339110 abstract "Abstract The condensed form of neural extracellular matrix (ECM), perineuronal nets (PNNs), is predominantly associated with parvalbumin-expressing (PV+) interneurons in the cortex and hippocampus. PNNs are enriched in several lecticans, including neurocan (Ncan). A polymorphism in the human Ncan gene has been associated with alterations in hippocampus-dependent memory function, variation of prefrontal cortex structure, and a higher risk for schizophrenia or bipolar disorder. Ncan knockout (KO) mice show related behavioral abnormalities, such as hyperactivity. Here we focused on studying how dysregulation of Ncan specifically in the mPFC may affect cognitive and synaptic functions. Intracortical adeno-associated virus (AAV) delivery was used to express shRNA against Ncan. Analysis of PNNs in Ncan shRNA-injected mice revealed a reduction in PNNs labelling by Wisteria floribunda agglutinin (WFA) around PV+ interneurons. Reduced Ncan expression resulted in a loss of the mPFC-dependent temporal order recognition and impairment of reversal spatial learning in a labyrinth (dry maze) task. As a potential synaptic substrate of these cognitive abnormalities, we report a robust reduction in the perisomatic GABAergic innervation of PV+ cells in Ncan KO and Ncan shRNA-injected mice. We also observed an increase in the density of vGLUT1-immunopositive synaptic puncta in the neuropil of Ncan shRNA-injected mice, which was, however, compensated in Ncan KO mice. Thus, our findings highlight a functional role of Ncan in supporting perisomatic GABAergic inhibition, temporal order recognition memory and cognitive flexibility, as one of the important cognitive resources depleted in neuropsychiatric disorders. Contribution to the field In this study, we asked if the extracellular matrix proteoglycan neurocan (Ncan) plays a functional role in the prefrontal cortex (PFC) of mice. Using viral delivery and expression of shRNA to knock down the expression of Ncan in the PFC, we provide evidence that neuronal Ncan is essential for the maintenance of perineuronal nets enveloping perisomatic interneurons by influencing the expression of glycoepitopes stained with Wisteria floribunda agglutinin and by modulating mRNA expression levels of other PNNs constituents. At the behavioral level, the knockdown of Ncan in mPFC impaired the temporal order recognition memory and consolidation/retrieval of spatial memories after reversal learning in the dry maze task. At the synaptic level, we found that Ncan knockdown reduced perisomatic GABAergic innervation of perisomatic interneurons and increased the density of vGLUT1+ excitatory presynaptic terminals in the neuropil of the PFC. Moreover, knockdown of Ncan changed the expression levels of several genes involved in activity-dependent synaptic remodeling. In summary, we conclude that neuronal Ncan is essential for multiple cognitive flexibility-related synaptic and cognitive functions in the PFC." @default.
• W4366339110 created "2023-04-20" @default.
• W4366339110 creator A5013433379 @default.
• W4366339110 creator A5015886951 @default.
• W4366339110 creator A5017762187 @default.
• W4366339110 creator A5022873707 @default.
• W4366339110 creator A5051588979 @default.
• W4366339110 creator A5054049113 @default.
• W4366339110 creator A5057678663 @default.
• W4366339110 creator A5066244994 @default.
• W4366339110 creator A5090278762 @default.
• W4366339110 creator A5090413925 @default.
• W4366339110 date "2023-04-18" @default.
• W4366339110 modified "2023-10-17" @default.
• W4366339110 title "Depletion of neurocan in the prefrontal cortex impairs temporal order recognition, cognitive flexibility and perisomatic GABAergic innervation" @default.
• W4366339110 cites W1486380944 @default.
• W4366339110 cites W1599255496 @default.
• W4366339110 cites W1760881456 @default.
• W4366339110 cites W1965162553 @default.
• W4366339110 cites W1965564800 @default.
• W4366339110 cites W1965726907 @default.
• W4366339110 cites W1965970232 @default.
• W4366339110 cites W1970963122 @default.
• W4366339110 cites W1972625759 @default.
• W4366339110 cites W1977433237 @default.
• W4366339110 cites W1981553997 @default.
• W4366339110 cites W1983790532 @default.
• W4366339110 cites W1993476760 @default.
• W4366339110 cites W1995007954 @default.
• W4366339110 cites W1996403037 @default.
• W4366339110 cites W1997218140 @default.
• W4366339110 cites W2000696407 @default.
• W4366339110 cites W2003554016 @default.
• W4366339110 cites W2004840471 @default.
• W4366339110 cites W2004855796 @default.
• W4366339110 cites W2009794244 @default.
• W4366339110 cites W2011868036 @default.
• W4366339110 cites W2018576843 @default.
• W4366339110 cites W2019431145 @default.
• W4366339110 cites W2020013805 @default.
• W4366339110 cites W2022932966 @default.
• W4366339110 cites W2026832087 @default.
• W4366339110 cites W2030236179 @default.
• W4366339110 cites W2033205851 @default.
• W4366339110 cites W2033746917 @default.
• W4366339110 cites W2035174549 @default.
• W4366339110 cites W2039539900 @default.
• W4366339110 cites W2044233502 @default.
• W4366339110 cites W2048361100 @default.
• W4366339110 cites W2054982643 @default.
• W4366339110 cites W2058335733 @default.
• W4366339110 cites W2060784568 @default.
• W4366339110 cites W2061272951 @default.
• W4366339110 cites W2061497228 @default.
• W4366339110 cites W2065547544 @default.
• W4366339110 cites W2069502544 @default.
• W4366339110 cites W2070700741 @default.
• W4366339110 cites W2071867187 @default.
• W4366339110 cites W2072652550 @default.
• W4366339110 cites W2074133215 @default.
• W4366339110 cites W2077816975 @default.
• W4366339110 cites W2079850562 @default.
• W4366339110 cites W2081150698 @default.
• W4366339110 cites W2081655757 @default.
• W4366339110 cites W2083248528 @default.
• W4366339110 cites W2083301995 @default.
• W4366339110 cites W2083920907 @default.
• W4366339110 cites W2084617736 @default.
• W4366339110 cites W2087129491 @default.
• W4366339110 cites W2087404956 @default.
• W4366339110 cites W2087592242 @default.
• W4366339110 cites W2088333881 @default.
• W4366339110 cites W2088887263 @default.
• W4366339110 cites W2091187766 @default.
• W4366339110 cites W2092864556 @default.
• W4366339110 cites W2096385890 @default.
• W4366339110 cites W2102038155 @default.
• W4366339110 cites W2106489369 @default.
• W4366339110 cites W2116686272 @default.
• W4366339110 cites W2123320547 @default.
• W4366339110 cites W2123352893 @default.
• W4366339110 cites W2130512993 @default.
• W4366339110 cites W2133757476 @default.
• W4366339110 cites W2139087276 @default.
• W4366339110 cites W2141532154 @default.
• W4366339110 cites W2141616957 @default.
• W4366339110 cites W2156939977 @default.
• W4366339110 cites W2159022089 @default.
• W4366339110 cites W2167279371 @default.
• W4366339110 cites W2167916157 @default.
• W4366339110 cites W2180691148 @default.
• W4366339110 cites W2181693490 @default.
• W4366339110 cites W2290509322 @default.
• W4366339110 cites W2294342312 @default.
• W4366339110 cites W2308600021 @default.
• W4366339110 cites W2414380634 @default.
• W4366339110 cites W2420689037 @default.
• W4366339110 cites W2461584436 @default.
• W4366339110 cites W2486491604 @default.
• W4366339110 cites W2527558243 @default.

• next