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- W4366372506 endingPage "339.e7" @default.
- W4366372506 startingPage "324" @default.
- W4366372506 abstract "Transcription factors (TFs) control gene expression, often acting synergistically. Classical thermodynamic models offer a biophysical explanation for synergy based on binding cooperativity and regulated recruitment of RNA polymerase. Because transcription requires polymerase to transition through multiple states, recent work suggests that “kinetic synergy” can arise through TFs acting on distinct steps of the transcription cycle. These types of synergy are not mutually exclusive and are difficult to disentangle conceptually and experimentally. Here, we model and build a synthetic circuit in which TFs bind to a single shared site on DNA, such that TFs cannot synergize by simultaneous binding. We model mRNA production as a function of both TF binding and regulation of the transcription cycle, revealing a complex landscape dependent on TF concentration, DNA binding affinity, and regulatory activity. We use synthetic TFs to confirm that the transcription cycle must be integrated with recruitment for a quantitative understanding of gene regulation." @default.
- W4366372506 created "2023-04-21" @default.
- W4366372506 creator A5001482859 @default.
- W4366372506 creator A5011616524 @default.
- W4366372506 creator A5021408861 @default.
- W4366372506 creator A5023904642 @default.
- W4366372506 creator A5030626594 @default.
- W4366372506 creator A5056777354 @default.
- W4366372506 creator A5069922511 @default.
- W4366372506 creator A5085780797 @default.
- W4366372506 date "2023-04-01" @default.
- W4366372506 modified "2023-10-14" @default.
- W4366372506 title "Transcriptional kinetic synergy: A complex landscape revealed by integrating modeling and synthetic biology" @default.
- W4366372506 cites W1786449125 @default.
- W4366372506 cites W1973462980 @default.
- W4366372506 cites W1974202718 @default.
- W4366372506 cites W1980991473 @default.
- W4366372506 cites W1982886646 @default.
- W4366372506 cites W1984189354 @default.
- W4366372506 cites W1985265438 @default.
- W4366372506 cites W1987390450 @default.
- W4366372506 cites W1990854276 @default.
- W4366372506 cites W1992127633 @default.
- W4366372506 cites W2002149313 @default.
- W4366372506 cites W2008047506 @default.
- W4366372506 cites W2009520959 @default.
- W4366372506 cites W2012451954 @default.
- W4366372506 cites W2013298988 @default.
- W4366372506 cites W2016349604 @default.
- W4366372506 cites W2022891974 @default.
- W4366372506 cites W2031223032 @default.
- W4366372506 cites W2033777731 @default.
- W4366372506 cites W2037489737 @default.
- W4366372506 cites W2043789254 @default.
- W4366372506 cites W2045362835 @default.
- W4366372506 cites W2051167924 @default.
- W4366372506 cites W2054435912 @default.
- W4366372506 cites W2063807804 @default.
- W4366372506 cites W2068962163 @default.
- W4366372506 cites W2072467292 @default.
- W4366372506 cites W2081712178 @default.
- W4366372506 cites W2082874386 @default.
- W4366372506 cites W2086876386 @default.
- W4366372506 cites W2086893629 @default.
- W4366372506 cites W2087234479 @default.
- W4366372506 cites W2088286107 @default.
- W4366372506 cites W2091688007 @default.
- W4366372506 cites W2097035920 @default.
- W4366372506 cites W2098188064 @default.
- W4366372506 cites W2105512180 @default.
- W4366372506 cites W2105924780 @default.
- W4366372506 cites W2106014350 @default.
- W4366372506 cites W2106837952 @default.
- W4366372506 cites W2111472187 @default.
- W4366372506 cites W2111846924 @default.
- W4366372506 cites W2114040881 @default.
- W4366372506 cites W2114151755 @default.
- W4366372506 cites W2114540703 @default.
- W4366372506 cites W2124055046 @default.
- W4366372506 cites W2128029143 @default.
- W4366372506 cites W2132291850 @default.
- W4366372506 cites W2134735802 @default.
- W4366372506 cites W2135229538 @default.
- W4366372506 cites W2140754446 @default.
- W4366372506 cites W2141215651 @default.
- W4366372506 cites W2141688036 @default.
- W4366372506 cites W2144347639 @default.
- W4366372506 cites W2149080058 @default.
- W4366372506 cites W2151715550 @default.
- W4366372506 cites W2159844490 @default.
- W4366372506 cites W2167279371 @default.
- W4366372506 cites W2171710183 @default.
- W4366372506 cites W2345943684 @default.
- W4366372506 cites W2411596856 @default.
- W4366372506 cites W2462656404 @default.
- W4366372506 cites W2465833271 @default.
- W4366372506 cites W2566313315 @default.
- W4366372506 cites W2570668323 @default.
- W4366372506 cites W2575110262 @default.
- W4366372506 cites W2602000457 @default.
- W4366372506 cites W2661487373 @default.
- W4366372506 cites W2742197765 @default.
- W4366372506 cites W2762370213 @default.
- W4366372506 cites W2770530503 @default.
- W4366372506 cites W2784772328 @default.
- W4366372506 cites W2787185499 @default.
- W4366372506 cites W2809057270 @default.
- W4366372506 cites W2811106513 @default.
- W4366372506 cites W2896975129 @default.
- W4366372506 cites W2902825844 @default.
- W4366372506 cites W2908212329 @default.
- W4366372506 cites W2915040067 @default.
- W4366372506 cites W2922894427 @default.
- W4366372506 cites W2923931114 @default.
- W4366372506 cites W2937836868 @default.
- W4366372506 cites W2945278782 @default.
- W4366372506 cites W2946478090 @default.
- W4366372506 cites W2947039670 @default.