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• W4366378533 abstract "Single particle reconstruction has recently emerged in 3D fluorescence microscopy as a powerful technique to improve the axial resolution and the degree of fluorescent labeling. It is based on the reconstruction of an average volume of a biological particle from the acquisition of multiple views with unknown poses. Current methods are limited either by template bias, restriction to 2D data, high computational cost or lack of robustness to low fluorescent labeling. In this work, we propose a single particle reconstruction method dedicated to convolutional models in 3D fluorescence microscopy that overcomes these issues. We address the joint reconstruction and estimation of the poses of the particles, which translates into a challenging non-convex optimization problem. Our approach is based on a multilevel reformulation of this problem, and the development of efficient optimization techniques at each level. We demonstrate on synthetic data that our method outperforms the standard approaches in terms of resolution and reconstruction error, while achieving a low computational cost. We also perform successful reconstruction on real datasets of centrioles to show the potential of our method in concrete applications." @default.
• W4366378533 created "2023-04-21" @default.
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• W4366378533 date "2023-01-01" @default.
• W4366378533 modified "2023-10-14" @default.
• W4366378533 title "Fast and Robust Single Particle Reconstruction in 3D Fluorescence Microscopy" @default.
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• W4366378533 doi "https://doi.org/10.1109/tci.2023.3266996" @default.
• W4366378533 hasPublicationYear "2023" @default.
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