Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366439425> ?p ?o ?g. }
- W4366439425 abstract "Osteoarthritis (OA) is a chronic degenerative joint disease characterized by loss of joint function, which seriously reduces the quality of life of the elderly and imposes a heavy socioeconomic burden worldwide. Monotropein (MON), the main active ingredient of Morinda officinalis F.C. How, has exhibited therapeutic effects in different disease models. However, its potential effects on chondrocytes in an arthritic model remain unclear. This study aimed to evaluate the effects of MON in chondrocytes and a mouse model of OA, and explore the potential mechanisms.Murine primary chondrocytes were pretreated with 10 ng/ml interleukin (IL)-1β for 24 h to establish an in vitro model of OA, and then treated with different concentrations of MON (0, 25, 50 and 100 μM) for 24 h. The proliferation of the chondrocytes was assayed using ethynyl-deoxyuridine (EdU) staining. Immunofluorescence staining, western blotting and TUNEL staining were performed to assess the effects of MON on cartilage matrix degradation, apoptosis and pyroptosis. The mouse model of OA was constructed by surgical destabilization of the medial meniscus (DMM), and the animals were randomly divided into the sham-operated, OA and OA + MON groups. Following OA induction, the mice were given intraarticular injection of 100 μM MON or equal volume of normal saline twice a week for 8 weeks. The effects of MON on cartilage matrix degradation, apoptosis and pyroptosis were assessed as indicated.MON significantly accelerated the proliferation of chondrocytes, and inhibited cartilage matrix degradation, apoptosis and pyroptosis in the IL-1β-stimulated cells by blocking the nuclear factor-kappa B (NF-κB) signaling pathway. In the mouse model as well, MON treatment alleviated OA progression and promoted cartilage repair by inhibiting cartilage matrix degradation, and chondrocyte apoptosis and pyroptosis through the inactivation of the NF-κB signaling pathway. Furthermore, the MON-treated arthritic mice exhibited better articular tissue morphology and lower OARSI scores.MON alleviated OA progression by inhibiting cartilage matrix degradation, and the apoptosis and pyroptosis of chondrocytes via NF-κB pathway inactivation, and is a promising alternative for the treatment of OA." @default.
- W4366439425 created "2023-04-21" @default.
- W4366439425 creator A5004726461 @default.
- W4366439425 creator A5005531885 @default.
- W4366439425 creator A5006128221 @default.
- W4366439425 creator A5009331454 @default.
- W4366439425 creator A5010444377 @default.
- W4366439425 creator A5022239024 @default.
- W4366439425 creator A5046364934 @default.
- W4366439425 creator A5076620241 @default.
- W4366439425 creator A5086844372 @default.
- W4366439425 creator A5026393205 @default.
- W4366439425 date "2023-04-19" @default.
- W4366439425 modified "2023-10-07" @default.
- W4366439425 title "Monotropein attenuates apoptosis and pyroptosis in chondrocytes and alleviates osteoarthritis progression in mice" @default.
- W4366439425 cites W1561041814 @default.
- W4366439425 cites W2012787784 @default.
- W4366439425 cites W2043344789 @default.
- W4366439425 cites W2044431971 @default.
- W4366439425 cites W2058435889 @default.
- W4366439425 cites W2100261712 @default.
- W4366439425 cites W2103495251 @default.
- W4366439425 cites W2135919238 @default.
- W4366439425 cites W2277908459 @default.
- W4366439425 cites W2303100670 @default.
- W4366439425 cites W2560541542 @default.
- W4366439425 cites W2627048017 @default.
- W4366439425 cites W2740118744 @default.
- W4366439425 cites W2776618650 @default.
- W4366439425 cites W2788239455 @default.
- W4366439425 cites W2902807504 @default.
- W4366439425 cites W2961082130 @default.
- W4366439425 cites W2969810988 @default.
- W4366439425 cites W2971741260 @default.
- W4366439425 cites W2972897928 @default.
- W4366439425 cites W2975310690 @default.
- W4366439425 cites W2979872515 @default.
- W4366439425 cites W2987679659 @default.
- W4366439425 cites W2990473002 @default.
- W4366439425 cites W2996365566 @default.
- W4366439425 cites W3000229516 @default.
- W4366439425 cites W3005090739 @default.
- W4366439425 cites W3015367575 @default.
- W4366439425 cites W3036138949 @default.
- W4366439425 cites W3044600719 @default.
- W4366439425 cites W3044835983 @default.
- W4366439425 cites W3088959920 @default.
- W4366439425 cites W3089646414 @default.
- W4366439425 cites W3091390800 @default.
- W4366439425 cites W3095012295 @default.
- W4366439425 cites W3108356963 @default.
- W4366439425 cites W3115872251 @default.
- W4366439425 cites W3120339302 @default.
- W4366439425 cites W3121427554 @default.
- W4366439425 cites W3128653999 @default.
- W4366439425 cites W3136319216 @default.
- W4366439425 cites W3138493921 @default.
- W4366439425 cites W3142080073 @default.
- W4366439425 cites W3153298114 @default.
- W4366439425 cites W3215729300 @default.
- W4366439425 cites W4200459954 @default.
- W4366439425 cites W4205899910 @default.
- W4366439425 cites W4220772909 @default.
- W4366439425 cites W4232024554 @default.
- W4366439425 cites W4248592944 @default.
- W4366439425 doi "https://doi.org/10.1186/s13020-023-00748-2" @default.
- W4366439425 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37076903" @default.
- W4366439425 hasPublicationYear "2023" @default.
- W4366439425 type Work @default.
- W4366439425 citedByCount "0" @default.
- W4366439425 crossrefType "journal-article" @default.
- W4366439425 hasAuthorship W4366439425A5004726461 @default.
- W4366439425 hasAuthorship W4366439425A5005531885 @default.
- W4366439425 hasAuthorship W4366439425A5006128221 @default.
- W4366439425 hasAuthorship W4366439425A5009331454 @default.
- W4366439425 hasAuthorship W4366439425A5010444377 @default.
- W4366439425 hasAuthorship W4366439425A5022239024 @default.
- W4366439425 hasAuthorship W4366439425A5026393205 @default.
- W4366439425 hasAuthorship W4366439425A5046364934 @default.
- W4366439425 hasAuthorship W4366439425A5076620241 @default.
- W4366439425 hasAuthorship W4366439425A5086844372 @default.
- W4366439425 hasBestOaLocation W43664394251 @default.
- W4366439425 hasConcept C105702510 @default.
- W4366439425 hasConcept C142724271 @default.
- W4366439425 hasConcept C17172800 @default.
- W4366439425 hasConcept C185592680 @default.
- W4366439425 hasConcept C190283241 @default.
- W4366439425 hasConcept C196795494 @default.
- W4366439425 hasConcept C203014093 @default.
- W4366439425 hasConcept C204787440 @default.
- W4366439425 hasConcept C2776164576 @default.
- W4366439425 hasConcept C2777077863 @default.
- W4366439425 hasConcept C2780550940 @default.
- W4366439425 hasConcept C2781403057 @default.
- W4366439425 hasConcept C31573885 @default.
- W4366439425 hasConcept C55493867 @default.
- W4366439425 hasConcept C71924100 @default.
- W4366439425 hasConcept C98274493 @default.
- W4366439425 hasConceptScore W4366439425C105702510 @default.
- W4366439425 hasConceptScore W4366439425C142724271 @default.