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• W4366442958 abstract "Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Control of neurotransmission efficacy is central to theories of how the brain computes and stores information. Presynaptic G-protein coupled receptors (GPCRs) are critical in this problem as they locally influence synaptic strength and can operate on a wide range of time scales. Among the mechanisms by which GPCRs impact neurotransmission is by inhibiting voltage-gated calcium (Ca2+) influx in the active zone. Here, using quantitative analysis of both single bouton Ca2+ influx and exocytosis, we uncovered an unexpected non-linear relationship between the magnitude of action potential driven Ca2+ influx and the concentration of external Ca2+ ([Ca2+]e). We find that this unexpected relationship is leveraged by GPCR signaling when operating at the nominal physiological set point for [Ca2+]e, 1.2 mM, to achieve complete silencing of nerve terminals. These data imply that the information throughput in neural circuits can be readily modulated in an all-or-none fashion at the single synapse level when operating at the physiological set point. Editor's evaluation In this important study Cook and Ryan find that at physiological temperatures, small changes in extracellular calcium concentrations shift individual presynaptic terminals between active and inactive states. This discovery is extended to the action of inhibitory synaptic modulation via GABA, emphasizing potentially broad relevance for our understanding of how synaptic transmission is stabilized and modulated throughout the brain. The evidence for the observed phenomenon is compelling, given the use of multiple functional reporters and rigorous analyses. https://doi.org/10.7554/eLife.83530.sa0 Decision letter Reviews on Sciety eLife's review process Introduction GPCRs serve a key control function in the brain, modifying the efficacy or time course of neurotransmission through pre- and postsynaptic mechanisms (Nadim and Bucher, 2014; Lovinger et al., 2022). Ligands of GPCRs are often secreted near synapses and exert their effects locally (van den Pol, 2012; van Westen et al., 2021; Ding et al., 2019). They can thereby modify region-specific synaptic properties and change the weighting of targeted synapses, in turn altering local computation. A canonical mode of presynaptic modulation occurs via the binding of voltage-gated calcium channels (VGCCs) by G-protein βγ subunits after they dissociate from the trimeric G-protein complex following GPCR activation (Herlitze et al., 1996). The impact of GPCR-mediated inhibition of VGCCs on neurotransmission (Kreitzer and Regehr, 2000; Yamada et al., 1999) is amplified by the exquisite sensitivity of exocytosis to the magnitude of Ca2+ influx, which underlies the ability to rapidly change exocytotic rates at sites of neurotransmitter release. This sensitivity is driven by the cooperative binding of multiple Ca2+ ions to one or more Ca2+ sensors that form core elements of the exocytic machinery (Heidelberger et al., 1994; Schneggenburger and Neher, 2000; Bollmann et al., 2000; Ariel and Ryan, 2010). Modifying the open probability of VGCCs, therefore, serves as a potent means of presynaptic control, as small changes in Ca2+ influx can lead to large changes in neurotransmitter release. The impact of Ca2+ on exocytosis depends on local changes in Ca2+ concentration near the active zone, which in turn depend on the external Ca2+ concentration bathing the nerve terminal (Dittman and Ryan, 2019). Thus, the steepness of the relationship between exocytosis rates and Ca2+ influx depends crucially on resting [Ca2+]e. In vivo, plasma [Ca2+] is strictly regulated, and the CNS is very sensitive to plasma [Ca2+] perturbations, with seizures precipitated by hypocalcemia and psychiatric symptoms progressing to lethargy or coma with worsening hypercalcemia (Riggs, 2002). Feedback loops controlled by Ca2+ sensors of the parathyroid gland act to maintain plasma [Ca2+] at a set point of 1.2 mM in most mammals (Hannan et al., 2019). Here, we used quantitative optical tools to investigate the sensitivity of exocytosis rates at the single-synapse level when operating at the physiological set point for [Ca2+]e. In a narrow range of [Ca2+]e near the physiologic concentration, our experiments revealed an unexpected sub-proportionality of Ca2+ influx relative to [Ca2+]e, indicating that a minimal Ca2+ entry is needed to sustain robust Ca2+ influx during AP firing. We show that three different approaches to modestly decrease Ca2+ influx, (1) blocking a fraction of Ca2+ channels, (2) lowering [Ca2+]e, or (3) application of a GPCR agonist, baclofen, known to reduce Ca2+ influx lead to complete silencing of a subset of nerve terminals. Thus, in this operating regime, decreasing presynaptic Ca2+ current may act as a digital switch to eliminate single bouton synaptic throughput. Results Ca2+ influx into nerve terminals is not 1:1 proportional to [Ca2+]e near the physiologic set point In order to examine how Ca2+ influx driven by action potentials (APs) at nerve terminals is impacted by changes in [Ca2+]e, we used the genetically-encoded Ca2+ indicator physin-GCaMP6f transfected sparsely into primary cultures of dissociated rat hippocampal neurons (Figure 1). We measured the changes in the GCaMP6f signal in response to a 20 AP burst stimulus (20 Hz) at 0.8, 1.2, and 2.0 mM [Ca2+]e (Figure 1A and B). Signals were corrected for the local abundance of the protein by determining the GCaMP6f fluorescence under saturating Ca2+ binding (determined following ionomycin application) and converted to absolute intracellular Ca2+ concentrations ([Ca2+]i, see methods). Previous studies investigating the dependency of Δ[Ca2+]i to [Ca2+]e in nerve terminals of the CNS found the relationship obeyed a simple saturable pore model with a Kd of [Ca2+]e ~2.5 mM (Ariel and Ryan, 2010; Schneggenburger et al., 1999). This model predicts that changes in Δ[Ca2+]i will exhibit 1-to-1 proportionality to [Ca2+]e at concentrations well below Kd, which is substantially higher than the physiologic set point ([Ca2+]e = 1.2 mM). One important confound, however, is that previous measurements were not carried out at physiological temperatures. Here, where the temperature was set to 37 °C, we observed an unexpected sub-proportional relationship of Δ[Ca2+]i to [Ca2+]e as the concentration was lowered below 2.0 mM (Figure 1B and C). The magnitude of difference between the expected and experimentally measured Δ[Ca2+]i was greater at [Ca2+]e 0.8 mM compared to 1.2 mM, highlighting that this discrepancy manifests most prominently below the set point (Figure 1C). A linear extrapolation from the mean Δ[Ca2+]i measurements relative to [Ca2+]e predicts the cessation of Ca2+ entry into nerve terminals at [Ca2+]e of 0.47 mM (Figure 1C). Experiments carried out using [Ca2+]e = 0.4 mM confirm that there is almost no measurable residual Ca2+ influx under these conditions (estimated Δ[Ca2+]~1.6 nM; Figure 1—figure supplement 1), far below the value expected (10.4 nM) if Ca2+ influx obeyed a simple linear proportionality. Figure 1 with 1 supplement see all Download asset Open asset Unexpected sub-proportionality in dependence of presynaptic calcium (Ca2+) influx on [Ca2+]e. (A) Schematic of the experimental protocol. Neurons are stimulated with a brief action potential (AP) train (20 AP in 1 s) at three different [Ca2+]e centered on the physiologic set point of 1.2 mM. Ionomycin, a Ca2+ ionophore, is administered at the end of the experiment to convert fluorescence into absolute [Ca2+]i. (B) An axon from a neuron expressing GCaMP6f localized to nerve terminals by synaptophysin (physin-GCaMP) at rest (top) and a difference image showing the response to 20 AP (bottom). The difference image is the mean peak (five frames) subtracted by the mean baseline (49 frames) in [Ca2+]e 2 mM. For display, a representative subset of terminals was highlighted. Scale bar is 10 µm. (C) Traces of responses to 20 AP from the neuron shown in B, with [Ca2+]e color-coded as in A. Expected Δ[Ca2+]i at 0.8 and 1.2 mM is represented by dashed lines and calculated as a change in influx relative to 2.0 mM proportional to the ratios of [Ca2+]e. Arrow bars show the difference between expected and measured Δ[Ca2+]i. Traces are mean, with error (SEM) represented by gray lines in only pre-stimulus and peak frames for clarity (n=211 nerve terminals). (D) Summary of Δ[Ca2+]i as a function of [Ca2+]e (mean ± 95%CI, n=9 neurons). The x-axis intercept for measured changes in Δ[Ca2+]i is 0.47 mM. *p<0.05, one-sample t-test compared to the expected value. Modest changes of [Ca2+]e near the physiologic set point controls the proportion of silent nerve terminals The above measurements represent ensemble averages of all terminals with a measurable signal following the saturation of physin-GCaMP. To further probe the apparent non-zero threshold of [Ca2+]e that enables Ca2+ entry, we assessed physin-GCaMP responses at individual nerve terminals with varying [Ca2+]e (Figure 2). Surprisingly, we found that a subset of terminals exhibited an all-or-none response to lowering [Ca2+]e below the physiologic set point such that Δ[Ca2+]i is selectively abolished (Figure 2B). To rigorously quantify this behavior, we employed a modest cutoff of one standard deviation of the pre-stimulus noise above the mean fluorescence to separate responding terminals from those without measurable synaptic activity, termed silent (Moulder et al., 2008; Altrock et al., 2003; Kim and Ryan, 2010). This definition of presynaptic silencing has been used previously in neurons expressing GCaMP3, a less sensitive indicator than GCaMP6f, and compared to the low-affinity, synthetic Ca2+ dye Magnesium Green with an excellent agreement, indicating that GCaMP sensors can reliably distinguish responding and silent terminals despite their non-linear Ca2+ dependence (Kim and Ryan, 2013). To evaluate the effectiveness of our thresholding, we compared cumulative frequency curves of ΔF for terminals separated into silent and responding populations (Figure 2—figure supplement 1). Responding terminals exhibit a clear shift towards higher ΔF with increasing [Ca2+]e, as expected, but the distribution of silent terminals was invariant with respect to [Ca2+]e. This analysis demonstrates that silent terminals are appropriately classified as incapable of generating detectable Ca2+ influx that would otherwise scale with [Ca2+]e. We observed that silencing occurred despite robust Δ[Ca2+]i at higher [Ca2+]e and even if synaptic neighbors of a terminal exhibited persistent Ca2+ entry at a lower concentration (Figure 2A and B), suggesting the impact of [Ca2+]e on neuronal function is exerted at the single synapse level. To further evaluate whether silencing may be driven by changes in the excitability of individual branches of neurons, we expressed GCaMP6f in the cytosol to clearly label the axon and marked terminals by co-expression of mRuby-synapsin (Figure 2—figure supplement 1A, B). We limited our analysis to regions unambiguously representing a single branch that had at least 10 terminals to provide a robust sample. These experiments showed that individual axonal branches have both silent and responding terminals (Figure 2—figure supplement 1C, D), indicating that branch point failure cannot account for [Ca2+]e mediated silencing. Figure 2 with 2 supplements see all Download asset Open asset Selective nerve terminal silencing is caused by changing [Ca2+]e around the physiologic set point. (A) Pre-stimulus average (left) and difference images (right) of AP stimulation (20 APs in 1 s) of a neuron expressing physin-GCaMP. The difference image is the mean peak (five frames) subtracted by the mean baseline (49 frames) at [Ca2+]e 2.0 and 0.8 mM. For display, a subset of representative terminals was selected and a Gaussian convolution with the radius of 1 pixel was applied. (B) Traces of the terminals indicated in A, shown as ΔF, demonstrate selective silencing (upper row compared to lower row) of a subset of terminals. Dotted line is the pre-stimulus mean fluorescence for each terminal. Across a population of neurons (Figure 3), we found substantial heterogeneity in the proportion of silent terminals (coefficient of variation was 38%, 56%, and 57% for [Ca2+]e 0.8, 1.2, and 2.0 mM, respectively) but a strikingly disproportionate increase in the mean proportion of silencing below 1.2 mM (Figure 3C). The change in percentage of silencing versus change in [Ca2+]e was 48.9% mM–1 transitioning from 1.2 to 0.8 mM compared to 19.0% mM–1 from 2.0 to 1.2 mM (Figure 3C). The change in percentage of silencing versus change in [Ca2+]e was 48.9% mM–1 transitioning from 1.2 to 0.8 mM compared to 19.0% mM–1 from 2.0 to 1.2 mM (Figure 3C). We separately tested the proportion of silencing when transitioning from 1.2 mM to 0.4 mM, observing that nearly all terminals (93%) are silent in [Ca2+]e 0.4 mM (Figure 3—figure supplement 1). Here, the coefficient of variation, 5%, was substantially lower (32% in paired measurements in [Ca2+]e 1.2 mM), demonstrating this level of [Ca2+]e potently and consistently shuts down presynaptic function. The change in percentage of silencing versus change in [Ca2+]e transition between 0.8 mM to 0.4 mM is 73.3% mM–1, again highlighting the steep dependence of silencing on [Ca2+]e below the physiologic [Ca2+]e. As expected, responding terminals demonstrated Δ[Ca2+]i that scaled with [Ca2+]e (Figure 3D). Nerve terminal silencing was reversible as the proportion of silent terminals before and after switching [Ca2+]e from 2.0 mM to 0.8 mM was unchanged (Figure 3—figure supplement 2A, B). Figure 3 with 2 supplements see all Download asset Open asset Silencing of Ca2+ influx into nerve terminals is potently modulated by changes in [Ca2+]e about the physiologic set point. (A) Diagram of the experimental protocol. (B) Example traces of single nerve terminals expressing physin-GCaMP, demonstrating persistent responsiveness (1) or silencing (2) with lowering of Ca2+ below physiologic extracellular levels. Dotted line is pre-stimulus mean fluorescence (ΔF=0). (C) The percentage of silent nerve terminals. Black dots are mean, error bars are SEM, and gray dots are individual cells. (D) Summary of Δ[Ca2+]i of responding terminals. Dots are individual cells, bars are mean, and error bars are SEM. (C) and (D) were analyzed with one-way ANOVA and Tukey’s post-test for multiple comparisons, *p<0.05, **p<0.01, ***p<0.001, n=9. The impact of [Ca2+]e on synaptic silencing is exerted through the global reduction in Ca2+ entry irrespective of VGCC subtype Because silencing of Ca2+ influx occurred in a variable proportion of terminals in each neuron, we next investigated whether this selectivity is mediated by the relative expression of VGCC subtypes. Ca2+ influx at hippocampal nerve terminals occurs predominantly via N- and P/Q-type VGCCs, with substantial heterogeneity between neurons in their proportional contribution to Ca2+ influx (Ariel et al., 2012; Ermolyuk et al., 2013; Hoppa et al., 2012). Previously, cyclin-dependent kinase 5 (CDK5) was shown to silence a subpopulation of nerve terminals, an effect mediated by N-type channels only (Kim and Ryan, 2013). We, therefore, hypothesized that N-type channels may exhibit a greater propensity to [Ca2+]e-driven silencing as compared to P/Q-type channels, accounting for neuronal variability in the proportion of silent terminals. To examine whether [Ca2+]e-mediated silencing exhibits subtype selectivity, we utilized ω-conotoxin-GVIA and ω-agatoxin IVA, potent toxins that specifically inhibit N- and P/Q-type channels, respectively (Figure 4A). The R-type toxin, SNX-482, was employed in these experiments to exclude the minor but possibly confounding contribution of these channels to overall Ca2+ influx (Ermolyuk et al., 2013). As previously demonstrated, the contribution of presynaptic VGCC subtypes to Δ[Ca2+]i is variable between hippocampal neurons (Figure 4B and C; Ariel et al., 2012). A subset of terminals is silenced with acute inhibition of either subtype, but the isolation of N-type channels leads to greater proportions of silent terminals (Figure 4B and D). To further investigate whether a difference exists between subtypes in the likelihood of silencing, we compared Ca2+ entry in responding terminals to the proportion of silent terminals at each tested [Ca2+]e (Figure 4C and D). This analysis reveals a non-linear relationship, such that lowering of absolute Ca2+ influx steeply increases the fraction of silent terminals. The comparison of Δ[Ca2+]i to silencing was well described with a Hill equation (r2 = 0.99, Kd = 25 nM, n = –1.79) and closely matched the relationship observed without toxin application (r2 = 0.77, Kd = 23 nM, n = –1.46; Figure 4E). This analysis indicates that the proportion of silent nerve terminals is critically related to global absolute Ca2+ influx and that differences between N- and P/Q-type channels are driven primarily by the impact that acute inhibition has on Ca2+ entry. Figure 4 Download asset Open asset The proportion of nerve terminal silencing in neurons is related to absolute calcium (Ca2+) influx irrespective of CaV2 subtype. (A) Diagram of the experimental protocol applied to neurons expressing physin-GCaMP. (B) The percentage of silent nerve terminals before and after toxin application to isolate a CaV2 subtype at [Ca2+]e 2 mM. (C) Summary of Δ[Ca2+]i in responding terminals after toxicologic isolation of N-type or P/Q-type channels. (D) Comparison of the effects of toxin application to isolate CaV2 subtypes on silencing (upper graph, shown on the left axis) and Ca2+ influx (lower graph, shown on the right axis). For B-D, symbols are the mean of individual neurons, lines and bars are the mean of all cells, and error bars are SEM. (B) and (C) were analyzed with a paired t-test, and D was analyzed with unpaired t-test. *p<0.05, ***p<0.001, n=6 for N-type and n=7 for P/Q-type. (E) The percentage of silent terminals plotted against Δ[Ca2+]i in responding terminals in control (black symbols) and following toxin application at varying [Ca2+]e. Symbols are mean and error bars are SEM. Data are fit with a Hill model, with the maximum at Δ[Ca2+]i = 0 constrained to 100%. (F) Relationship of the percentage of silent terminals to Δ[Ca2+]i in responding terminals with varying [Ca2+]e in neurons not treated with CaV2 toxins. Dots are the mean of individual cells from neurons presented in Figure 2. Data are fit as in E. Changing [Ca2+]e near the physiologic set point potently controls the silencing of SV recycling and glutamate exocytosis at neurotransmitter release sites Our observation of steep changes in the proportion of nerve terminals in which Ca2+ influx was silenced by lowering [Ca2+]e below the physiologic set point led us to investigate the impact of [Ca2+]e on neurotransmitter release. We first addressed silencing of SV recycling with pHluorin, a pH-sensitive fluorescent protein (Miesenböck et al., 1998; Sankaranarayanan et al., 2000), tagged to vGLUT1 (vGpH), which is quenched in the acidic lumen of synaptic vesicles but fluoresces with exocytosis and exposure to the relatively alkaline extracellular environment (Voglmaier et al., 2006; Figure 5A–C). We employed a robust stimulus of 200 AP (20 Hz) to distinguish responding from silent terminals (Kim and Ryan, 2013; Kim and Ryan, 2009; Figure 5A and C). Similar to synaptic Ca2+ influx, silencing of SV recycling occurred at a substantial subset of nerve terminals (~25 to 55%, Figure 5D). The proportion of silent terminals was heterogeneous across neurons but the mean and range at each [Ca2+]e were similar to the degree of silencing observed with Δ[Ca2+]i (Figure 3C). As expected, the increase in SV recycling in responding terminals scaled with [Ca2+]e (Figure 5E). Thus, as with Ca2+ influx, SV recycling is modulated in an all-or-none manner by modest changes in [Ca2+]e around the physiological set point ([Ca2+]e = 1.2 mM). Figure 5 Download asset Open asset Exocytosis of SVs exhibits [Ca2+]e-driven silencing. (A) Diagram of the experimental protocol. (B) Segment of an axon expressing vGpH, showing a difference in fluorescence in nerve terminals revealed by the application of NH4Cl 50 mM. Scale bar is 10 µm, and the calibration is maximum-normalized fluorescence intensity. (C) SV recycling of individual nerve terminals indicated in B demonstrating selective silencing at [Ca2+]e 0.8 mM in (1) compared to the persistent response in (2). Traces of different [Ca2+]e are color-coded as in A. (D) Percentage of silent nerve terminals. Black dots are mean, error bars are SEM, and gray dots are individual cells. (E) SV exocytosis of responding terminals in different [Ca2+]e. Dots are individual neurons, bars are the mean, and error bars are SEM. (D) and (E) were analyzed with one-way ANOVA and Tukey’s post-test for multiple comparisons, *p<0.05, **p<0.01, ***p<0.01, n=9. We next sought to directly assess how [Ca2+]e regulates the silencing of glutamate release in the regime of stimulation with a single AP. To do so, we utilized iGluSnFR3 v857 (iGluSnFR), a genetically-encoded, fluorescent biosensor of extracellular glutamate concentrations with an excellent signal-to-noise ratio and temporal resolution enabling quantification of glutamate release from one AP at individual nerve terminals (Aggarwal et al., 2022). Co-expression of mRuby-synapsin allowed measurements to be localized to neurotransmitter release sites (Figure 6B); however, because iGluSnFR is anchored by glycophosphatidylinositol (GPI) to the cell surface, the indicator does not necessarily discriminate between glutamate released by the transfected cell or neighboring terminals from non-transfected neurons (Aggarwal et al., 2022). Accordingly, while fluorescent signals at mRuby-synapsin puncta may receive contributions from additional terminals, silent terminals are definitively assigned as inactive. With this approach, we found glutamate exocytosis evoked by a single AP exhibited higher proportions of silencing at neurotransmitter release sites as [Ca2+]e was lowered, again demonstrating a marked increase in silencing as the concentration decreased below the physiologic set point (Figure 6C and D). Thus, using these two fluorescent biosensors quantifying neurotransmitter handling under different stimulation regimes demonstrates that [Ca2+]e is an important regulator of presynaptic function, differentially dictating across synapses and neurons whether neurotransmitter will be released. Figure 6 Download asset Open asset Glutamate release elicited by 1 AP demonstrates [Ca2+]e-driven silencing at neurotransmitter release sites. (A) Diagram of the experimental protocol. Responses of iGluSnFR-expressing neurons were averaged over 15 single AP trials delivered in ~20 s intervals. (B) Difference image (peak minus baseline fluorescence) of an axon expressing iGluSnFR (cyan, upper) stimulated with 1 AP in [Ca2+]e 1.2 mM, with terminals marked by mRuby-synapsin (magenta, middle; merge lower). Scale bar 20 µm. (B) Traces of iGluSnFR measured at nerve terminals indicated in B, illustrating differential silencing as [Ca2+]e is lowered. Traces of different [Ca2+]e are color-coded as in A. (D) Percentage of silent nerve terminals. Black dots are mean, error bars are SEM, and gray dots are individual cells. (E) Glutamate release, quantified as ΔF/Fmax, in responding terminals as [Ca2+]e is decreased. Dots are the mean of individual cells, bars are mean, and error bars ± SEM. (D) and (E) were analyzed with one-way ANOVA and Tukey’s post-test for multiple comparisons. *p<0.05, **p<0.01, ***p<0.001, n=8. GPCRs that lower synaptic Ca2+ currents similarly modulate the proportion of silent nerve terminals The selective silencing of Ca2+ entry and neurotransmitter release in subpopulations of nerve terminals by globally reducing Ca2+ influx may be an important mechanism determining the behavior of synapses to biological processes affecting Ca2+ currents (ICa). For instance, a subset of important presynaptic GPCRs affects neurotransmission by lowering synaptic Ca2+ influx (Dolphin, 2003). One such canonical mechanism is the agonism of the GABAB receptor (GABABR), which lowers ICa by approximately 50% via interactions of the Gβγ subunit with presynaptic VGCCs (Mintz and Bean, 1993; Herlitze et al., 1996; Isaacson, 1998; Laviv et al., 2011). Thus, we hypothesized that GABABR-mediated decreases in ICa will significantly increase the degree of nerve terminal silencing at the physiologic set point of [Ca2+]e. We compared the effect of baclofen, a GABABR agonist, on the silencing of Ca2+ influx and SV recycling using physin-GCaMP and vGpH, respectively (Figure 7A–C). Consistent with our proposed mechanism, agonizing GABABR at [Ca2+]e 1.2 mM caused an additional ~20% of terminals to become silent in both Ca2+ influx and SV recycling (Figure 7D), similar to the proportional increase we observed decreasing [Ca2+]e to 0.8 mM. However, agonism with baclofen in [Ca2+]e 2.0 mM led to a substantially lower increase in the proportion of silencing (7.6 ± 3.4% vs 20.5 ± 4.8%, p=0.017; Figure 7—figure supplement 1A, B). Thus, GPCR-mediated silencing was dependent on [Ca2+]e such that the impact was far greater operating at the physiologic [Ca2+]e. These results confirm that GPCR-mediated modulation acting presynaptically to decrease ICa can silence a substantial proportion of nerve terminals. Figure 7 with 1 supplement see all Download asset Open asset Agonism of GABABR with baclofen leads to silencing of calcium (Ca2+) influx and SV exocytosis. (A) Diagram of the experimental protocol. (B) Representative traces of silencing of Ca2+ influx and SV exocytosis resulting from agonism of GABABR with baclofen in single nerve terminals expressing physin-GCaMP or vGpH, respectively. (C) Δ[Ca2+]i and ΔF/ΔFNH4Cl before and following the application of baclofen in responding terminals. (D) The percentage of silent terminals before and following treatment with baclofen was measured in physin-GCaMP and vGpH-expressing neurons (see labels above). For (C) and (D), dots are individual neurons, bars are mean of all neurons, and error bars are SEM. (C) and (D) were analyzed with a paired t-test, *p<0.05, **p<0.01, n=8 for physin-GCaMP, and n=9 for vGpH. Discussion The flux of Ca2+ in neurons plays a central role in neurotransmission by triggering the exocytosis of SVs, thereby releasing neurotransmitters into the synaptic cleft (Dittman and Ryan, 2019). Uncovering the mechanisms regulating Ca2+ entry into nerve terminals is therefore critical to understanding the molecular underpinnings of CNS function. The relationship between Ca2+ influx and the exocytotic rate is highly cooperative (Heidelberger et al., 1994; Schneggenburger and Neher, 2000; Bollmann et al., 2000; Ariel and Ryan, 2010), highlighting the amplification of neurotransmitter release caused by changes in Δ[Ca2+]i. Fewer studies have sought to determine the effect of [Ca2+]e on Δ[Ca2+]i and, to our knowledge, none have examined this in the range encompassing the physiological set point for [Ca2+]e at physiological temperature. In general, the narrowing of the AP at warmer temperatures (Hodgkin and Katz, 1949; Yu et al., 2012) results in lowering of Ca2+ influx during AP firing, so additionally studying lower [Ca2+]e results in a much lower regime of Δ[Ca2+]i. Previous attempts (carried out at sub-physiological temperatures) modeled this relationship with Michaelis-Menten kinetics, where Δ[Ca2+]i obeyed a simple saturation of VGCCs, but predicted a simple 1:1 relationship extrapolating to lower [Ca2+]e (Ariel and Ryan, 2010; Schneggenburger et al., 1999). Here, we show an unexpected and impactful behavior of Ca2+ influx in the lower Δ[Ca2+]i regime, which is that small modulation of Ca2+ influx leads to dramatic silencing of individual nerve terminals. We discovered that a substantial subset of terminals (~40%) exhibited silencing of Ca2+ influx and SV exocytosis at physiologic [Ca2+]e, and this proportion increased steeply as [Ca2+]e was lowered to 0.8 mM and 0.4 mM (to ~60% and >90%, respectively). In comparison, the change in the proportion of silent terminals in the transition from [Ca2+]e 2.0 mM to 1.2 mM, a difference of ~15%, was smaller despite a larger step in [Ca2+]e. Previous estimates of synaptic silencing were drawn from experiments conducted at [Ca2+]e 2.0 mM and agree with our findings at this concentration (Altrock et al., 2003; Kim and Ryan, 2013; Moulder et al., 2004; Moulder et al., 2006). However, our results highlight that the proportion of silent terminals in cultured neurons is substantially higher under conditions of physiologic [Ca2+]e. Thus, [Ca2+]e is a crucial variable to consider in future studies examining synaptic silencing. The agreement of our findings in the silencing of both Δ[Ca2+]i and SV exocytosis indicate that silencing of Δ[Ca2+]i is driving the effect as opposed to an independent mechanism operating on SV exocytosis alone. The proportion of silencing across different neurons was steeply related to absolute Δ[Ca2+]i, such that lowering of Δ[Ca2+]i was associated with increased proportions of silent terminals. Interestingly, this relationship persisted despite the selective blockade of VGCC subtypes by potent toxins, demonstrating the propensity for silencing is not related to their relative distribution at nerve terminals. Due to this" @default.
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• W4366442958 title "Editor's evaluation: GABABR silencing of nerve terminals" @default.
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