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- W4366452053 abstract "Development of rapid in vitro cloning through axillary shoot proliferation from mature clump for the production of qualityplanting material has been described. Explants from 12-year old field grown clump of Bambusa vulgaris cv. Wamin (BVW) fromInstitute of Wood Science and Technology, Bangalore were used for initiation of cultures. Axillary bud break was achieved 95%of cultures in BVW on MS (Murashige and Skoog) liquid medium supplemented with additives and various combination of auxinsIAA, NAA(0.25mgl-1) and cytokinins viz., Kinetin, BAP (0.5-2.5mgl-1), TDZ (0.05-0.25mgl-1 ) . Additives ascorbic acid (50mgl-1),citric acid (25mgl-1) and cysteine (25mgl-1) were added during shoot initiation and shoot multiplication. The proliferated shoots (2-3 weeks old) showed rapid multiplication with 27-28 shoots/clump at fifth passage on MS liquid medium supplemented withadditives and BAP (5.0mgl-1). A clump of atleast 3 shoots were used for in vitro root induction in half- strength MS medium withNAA (1.0mgl-1). Field transfer of in vitro rooted plantlets were carried out by transferring the plantlets to pots containing sand:soil: FYM (farmyard manure) in 1:1:1 ratio, maintained in nursery at 30±2°C and relative humidity of 80±5%. Micropropagatedplants achieved a height of 30-35 cm with 3-4 tillers (shoots) with miniature rhizome within four months." @default.
- W4366452053 created "2023-04-22" @default.
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- W4366452053 date "2014-12-30" @default.
- W4366452053 modified "2023-10-07" @default.
- W4366452053 title "In vitro propagation of Bambusa vulgaris cv. Wamin by axillary shoot proliferation" @default.
- W4366452053 doi "https://doi.org/10.59415/ijfas.v3i4.64" @default.
- W4366452053 hasPublicationYear "2014" @default.
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