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- W4366550106 abstract "Abstract Mapping wheat genes, in the centromeric and pericentromeric regions (~2/3 rd of a given chromosome), poses a formidable challenge due to highly suppressed recombination. Using an example of compact spike locus ( C-locus) , this study provides an approach to precisely map wheat genes in the pericentromeric and centromeric regions that house ~30% of wheat genes. In Club-wheat, spike compactness is controlled by the dominant C-locus, but previous efforts have failed to localize it , on a particular arm of chromosome 2D. We integrated radiation hybrid (RH) and high-resolution genetic mapping to locate C-locus on the short arm of chromosome 2D. Flanking markers of the C-locus span a physical distance of 11.0 Mb (231.0-242 Mb interval) and contain only 11 high-confidence annotated genes. This work demonstrates the value of this integrated strategy in mapping dominant genes in the low-recombination regions of the wheat genome. A comparison of the mapping resolutions of the RH and genetic maps using common anchored markers indicated that the RH map provides ~9 times better resolution that the genetic map even with much smaller population size. This study provides a broadly applicable approach to fine-map wheat genes in regions of suppressed recombination." @default.
- W4366550106 created "2023-04-22" @default.
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- W4366550106 date "2023-04-20" @default.
- W4366550106 modified "2023-09-23" @default.
- W4366550106 title "Physical mapping of the wheat genes in low recombination regions: Radiation Hybrid mapping of the C-locus" @default.
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- W4366550106 doi "https://doi.org/10.21203/rs.3.rs-2786288/v1" @default.
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