Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366587716> ?p ?o ?g. }
- W4366587716 abstract "d-Allulose has many health-benefiting properties and therefore sustainably applied in food, pharmaceutical, and nutrition industries. The aldol reaction-based route is a very promising alternative to Izumoring strategy in d-allulose production. Remarkable studies reported in the past cannot get rid of by-product formation and costly purified enzyme usage. In the present study, we explored the glycerol assimilation by modularly assembling the d-allulose synthetic cascade in Escherichia coli envelop. We achieved an efficient whole-cell catalyst that produces only d-allulose from cheap glycerol feedstock, eliminating the involvement of purified enzymes. Detailed process optimization improved the d-allulose titer by 1500.00%. Finally, the production was validated in 3-L scale using a 5-L fermenter, and 5.67 g L−1 d-allulose was produced with a molar yield of 31.43%." @default.
- W4366587716 created "2023-04-23" @default.
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- W4366587716 date "2023-04-27" @default.
- W4366587716 modified "2023-09-24" @default.
- W4366587716 title "Engineering an efficient whole‐cell catalyst for <scp>d</scp>‐allulose production from glycerol" @default.
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- W4366587716 doi "https://doi.org/10.1002/biot.202200600" @default.
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- W4366587716 hasPublicationYear "2023" @default.
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