FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366602215> ?p ?o ?g. }

• W4366602215 endingPage "115323" @default.
• W4366602215 startingPage "115323" @default.
• W4366602215 abstract "Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) proteins are an innovative tool in molecular diagnostics owing to their high specificity and modularity for target nucleic acid sequences. However, the sequence-indiscriminate trans-cleavage activity of the Cas protein renders multiplex detection challenging. In this study, we developed a Cas12a-based multiplex detection system by designing blocker DNA complementary to reporter DNA, which enables the simultaneous detection of two genes with a single Cas protein in a single reaction. As a proof of concept, we chose high-risk human papillomavirus (HPV) 16 and 18 as the model targets and incorporated recombinase polymerase amplification (RPA) and transcription reactions to achieve high accuracy and sensitivity. Using the proposed system, we detected the genes of both HPV 16 and 18 down to 1 aM within 80 min under isothermal conditions. We validated the performance of the system in detecting genomic DNA from various cell lines and clinical samples from cervical cancer patients with high specificity. The proposed system facilitated rapid multiplex detection of high-risk HPVs in a single reaction tube with only Cas12a, thus representing a more user-friendly and economical alternative to previous Cas protein-based multiplex detection assays. The proposed system has considerable potential for point-of-care testing and could be expanded to detect various nucleic acid biomarkers." @default.
• W4366602215 created "2023-04-23" @default.
• W4366602215 creator A5016279934 @default.
• W4366602215 creator A5062434800 @default.
• W4366602215 creator A5079289762 @default.
• W4366602215 creator A5082192714 @default.
• W4366602215 creator A5082880112 @default.
• W4366602215 creator A5083737579 @default.
• W4366602215 creator A5085491969 @default.
• W4366602215 creator A5088927197 @default.
• W4366602215 date "2023-07-01" @default.
• W4366602215 modified "2023-10-16" @default.
• W4366602215 title "Cas12a/blocker DNA-based multiplex nucleic acid detection system for diagnosis of high-risk human papillomavirus infection" @default.
• W4366602215 cites W1976391205 @default.
• W4366602215 cites W2018196842 @default.
• W4366602215 cites W2050470199 @default.
• W4366602215 cites W2050480410 @default.
• W4366602215 cites W2117444879 @default.
• W4366602215 cites W2120932131 @default.
• W4366602215 cites W2131255017 @default.
• W4366602215 cites W2131527797 @default.
• W4366602215 cites W2169244146 @default.
• W4366602215 cites W2169257864 @default.
• W4366602215 cites W2346028445 @default.
• W4366602215 cites W2579254897 @default.
• W4366602215 cites W2606915822 @default.
• W4366602215 cites W2608617863 @default.
• W4366602215 cites W2741037975 @default.
• W4366602215 cites W2785904295 @default.
• W4366602215 cites W2787024379 @default.
• W4366602215 cites W2921110444 @default.
• W4366602215 cites W2973445868 @default.
• W4366602215 cites W2974267223 @default.
• W4366602215 cites W2981675356 @default.
• W4366602215 cites W2998321846 @default.
• W4366602215 cites W2998639792 @default.
• W4366602215 cites W3012071400 @default.
• W4366602215 cites W3015659943 @default.
• W4366602215 cites W3017334101 @default.
• W4366602215 cites W3028883492 @default.
• W4366602215 cites W3036593363 @default.
• W4366602215 cites W3087905712 @default.
• W4366602215 cites W3088014076 @default.
• W4366602215 cites W3112638533 @default.
• W4366602215 cites W3112640323 @default.
• W4366602215 cites W3115403348 @default.
• W4366602215 cites W3119666167 @default.
• W4366602215 cites W3186756286 @default.
• W4366602215 cites W3190958421 @default.
• W4366602215 cites W3195217732 @default.
• W4366602215 cites W3196730997 @default.
• W4366602215 cites W3198740632 @default.
• W4366602215 cites W3199668877 @default.
• W4366602215 cites W3206154855 @default.
• W4366602215 cites W4200024803 @default.
• W4366602215 cites W4213214232 @default.
• W4366602215 cites W4280546633 @default.
• W4366602215 cites W4283698466 @default.
• W4366602215 cites W4317528890 @default.
• W4366602215 cites W2985532529 @default.
• W4366602215 doi "https://doi.org/10.1016/j.bios.2023.115323" @default.
• W4366602215 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37079992" @default.
• W4366602215 hasPublicationYear "2023" @default.
• W4366602215 type Work @default.
• W4366602215 citedByCount "3" @default.
• W4366602215 countsByYear W43666022152023 @default.
• W4366602215 crossrefType "journal-article" @default.
• W4366602215 hasAuthorship W4366602215A5016279934 @default.
• W4366602215 hasAuthorship W4366602215A5062434800 @default.
• W4366602215 hasAuthorship W4366602215A5079289762 @default.
• W4366602215 hasAuthorship W4366602215A5082192714 @default.
• W4366602215 hasAuthorship W4366602215A5082880112 @default.
• W4366602215 hasAuthorship W4366602215A5083737579 @default.
• W4366602215 hasAuthorship W4366602215A5085491969 @default.
• W4366602215 hasAuthorship W4366602215A5088927197 @default.
• W4366602215 hasConcept C104317684 @default.
• W4366602215 hasConcept C126322002 @default.
• W4366602215 hasConcept C132455925 @default.
• W4366602215 hasConcept C139713532 @default.
• W4366602215 hasConcept C153911025 @default.
• W4366602215 hasConcept C24107716 @default.
• W4366602215 hasConcept C2781188995 @default.
• W4366602215 hasConcept C2994532551 @default.
• W4366602215 hasConcept C49105822 @default.
• W4366602215 hasConcept C54355233 @default.
• W4366602215 hasConcept C552990157 @default.
• W4366602215 hasConcept C70721500 @default.
• W4366602215 hasConcept C71924100 @default.
• W4366602215 hasConcept C84356384 @default.
• W4366602215 hasConcept C86803240 @default.
• W4366602215 hasConcept C90583042 @default.
• W4366602215 hasConcept C98108389 @default.
• W4366602215 hasConceptScore W4366602215C104317684 @default.
• W4366602215 hasConceptScore W4366602215C126322002 @default.
• W4366602215 hasConceptScore W4366602215C132455925 @default.
• W4366602215 hasConceptScore W4366602215C139713532 @default.
• W4366602215 hasConceptScore W4366602215C153911025 @default.
• W4366602215 hasConceptScore W4366602215C24107716 @default.

• next