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- W4366602806 abstract "Simple, low-cost, and accurate nucleic acid assay platforms hold great promise for point-of-care (POC) pathogen detection, disease surveillance, and control. Plasmonic photothermal polymerase chain reaction (PPT-PCR) is a powerful and efficient nucleic acid amplification technique, but it lacks a simple and convenient analysis method for POC applications. Herein, we propose a novel plasmonic cross-linking colorimetric PCR (PPT-ccPCR) assay by integrating plasmonic magnetic nanoparticle (PMN)-based PPT-PCR with gold nanoparticle (AuNP)-based cross-linking colorimetry. AuNPs form assembled structures with the PMNs in the presence of amplicons and collect in a magnetic field, resulting in color changes to the supernatant. Target DNA with concentrations as low as 5 copies/μL can be visually detected within 40 min. The achieved limit of detection was 1.8 copies/μL based on the absorption signals. This simple and sensitive strategy needs no expensive instrumentation and demonstrates high potential for POC detection while enabling further applications in clinical diagnostics." @default.
- W4366602806 created "2023-04-23" @default.
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- W4366602806 date "2023-04-21" @default.
- W4366602806 modified "2023-10-18" @default.
- W4366602806 title "Plasmonic Cross-Linking Colorimetric PCR for Simple and Sensitive Nucleic Acid Detection" @default.
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- W4366602806 doi "https://doi.org/10.1021/acs.nanolett.3c00533" @default.
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- W4366602806 hasPublicationYear "2023" @default.
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