FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366605421> ?p ?o ?g. }

• W4366605421 abstract "Background and Purpose Receptor activity‐modifying proteins (RAMPs) and melanocortin receptor accessory proteins (MRAPs) modulate expression and signalling of calcitonin and melanocortin GPCRs. Interactions with other GPCRs have also been reported. The cannabinoid receptors, CB 1 and CB 2 , and two putative cannabinoid receptors, GPR18 and GPR55, exhibit substantial intracellular expression and there are discrepancies in ligand responsiveness between studies. We investigated whether interactions with RAMPs or MRAPs could explain these phenomena. Experimental Approach Receptors and accessory proteins were co‐expressed in HEK‐293 cells. Selected receptors were studied at basal expression levels and also with enhanced expression produced by incorporation of a preprolactin signal sequence/peptide (pplss). Cell surface and total expression of receptors and accessory proteins were quantified using immunocytochemistry. Signalling was measured using cAMP (CAMYEL) and G protein dissociation (TRUPATH Gα 13 ) biosensors. Key Results MRAP2 enhanced surface and total expression of GPR18. Pplss‐GPR18 increased detection of cell surface MRAP2. MRAP1α and MRAP2 reduced GPR55 surface and total expression, correlating with reduced constitutive, but not agonist‐induced, signalling. GPR55, pplss‐CB 1 and CB 2 reduced detection of MRAP1α at the cell surface. Pplss‐CB 1 agonist potency was reduced by MRAP2 in Gα 13 but not cAMP assays, consistent with MRAP2 reducing pplss‐CB 1 expression. Some cannabinoid receptors increased RAMP2 or RAMP3 total expression without influencing surface expression. Conclusions and Implications Mutual influences on expression and/or function for specific accessory protein‐receptor pairings raises the strong potential for physiological and disease‐relevant consequences. Sequestration and/or hetero‐oligomerisation of cannabinoid receptors with accessory proteins is a possible novel mechanism for receptor crosstalk." @default.
• W4366605421 created "2023-04-23" @default.
• W4366605421 creator A5019310189 @default.
• W4366605421 creator A5029174750 @default.
• W4366605421 creator A5034461105 @default.
• W4366605421 creator A5035621970 @default.
• W4366605421 creator A5053643224 @default.
• W4366605421 creator A5053915349 @default.
• W4366605421 date "2023-06-01" @default.
• W4366605421 modified "2023-09-27" @default.
• W4366605421 title "RAMP and MRAP accessory proteins have selective effects on expression and signalling of the CB₁, CB₂, GPR18 and GPR55 cannabinoid receptors" @default.
• W4366605421 cites W1491382381 @default.
• W4366605421 cites W1504900766 @default.
• W4366605421 cites W1534915247 @default.
• W4366605421 cites W1535107724 @default.
• W4366605421 cites W153797087 @default.
• W4366605421 cites W1596388264 @default.
• W4366605421 cites W1819429514 @default.
• W4366605421 cites W1836729582 @default.
• W4366605421 cites W1975778365 @default.
• W4366605421 cites W1979569217 @default.
• W4366605421 cites W1989814102 @default.
• W4366605421 cites W1995622297 @default.
• W4366605421 cites W2002309357 @default.
• W4366605421 cites W2003063658 @default.
• W4366605421 cites W2006687559 @default.
• W4366605421 cites W2006707076 @default.
• W4366605421 cites W2010797522 @default.
• W4366605421 cites W2012034410 @default.
• W4366605421 cites W2015256174 @default.
• W4366605421 cites W2029003083 @default.
• W4366605421 cites W2031545914 @default.
• W4366605421 cites W2037768649 @default.
• W4366605421 cites W2037811574 @default.
• W4366605421 cites W2064056368 @default.
• W4366605421 cites W2071384116 @default.
• W4366605421 cites W2077049377 @default.
• W4366605421 cites W2079173661 @default.
• W4366605421 cites W2083214820 @default.
• W4366605421 cites W2084213487 @default.
• W4366605421 cites W2088979187 @default.
• W4366605421 cites W2098925719 @default.
• W4366605421 cites W2115824704 @default.
• W4366605421 cites W2123276616 @default.
• W4366605421 cites W2139870433 @default.
• W4366605421 cites W2140436003 @default.
• W4366605421 cites W2148639041 @default.
• W4366605421 cites W2155244384 @default.
• W4366605421 cites W2162312300 @default.
• W4366605421 cites W2177303033 @default.
• W4366605421 cites W2234854174 @default.
• W4366605421 cites W2298859064 @default.
• W4366605421 cites W2412327581 @default.
• W4366605421 cites W2412596082 @default.
• W4366605421 cites W2501047946 @default.
• W4366605421 cites W2501170245 @default.
• W4366605421 cites W2505162004 @default.
• W4366605421 cites W2516240197 @default.
• W4366605421 cites W2522224926 @default.
• W4366605421 cites W2566514911 @default.
• W4366605421 cites W2597703765 @default.
• W4366605421 cites W2614187876 @default.
• W4366605421 cites W2765091532 @default.
• W4366605421 cites W2765472374 @default.
• W4366605421 cites W2901738386 @default.
• W4366605421 cites W2907376444 @default.
• W4366605421 cites W2912011779 @default.
• W4366605421 cites W2913367166 @default.
• W4366605421 cites W2935826300 @default.
• W4366605421 cites W2936463751 @default.
• W4366605421 cites W2937393835 @default.
• W4366605421 cites W2978528228 @default.
• W4366605421 cites W2982702417 @default.
• W4366605421 cites W3008004932 @default.
• W4366605421 cites W3022812876 @default.
• W4366605421 cites W3043266434 @default.
• W4366605421 cites W3086755283 @default.
• W4366605421 cites W3087307869 @default.
• W4366605421 cites W3127619671 @default.
• W4366605421 cites W3163484416 @default.
• W4366605421 cites W3184729641 @default.
• W4366605421 cites W3199710005 @default.
• W4366605421 cites W3209576972 @default.
• W4366605421 cites W3215103241 @default.
• W4366605421 cites W4220763721 @default.
• W4366605421 cites W4282941439 @default.
• W4366605421 cites W4288046259 @default.
• W4366605421 cites W4361305713 @default.
• W4366605421 doi "https://doi.org/10.1111/bph.16095" @default.
• W4366605421 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37085333" @default.
• W4366605421 hasPublicationYear "2023" @default.
• W4366605421 type Work @default.
• W4366605421 citedByCount "0" @default.
• W4366605421 crossrefType "journal-article" @default.
• W4366605421 hasAuthorship W4366605421A5019310189 @default.
• W4366605421 hasAuthorship W4366605421A5029174750 @default.
• W4366605421 hasAuthorship W4366605421A5034461105 @default.
• W4366605421 hasAuthorship W4366605421A5035621970 @default.
• W4366605421 hasAuthorship W4366605421A5053643224 @default.
• W4366605421 hasAuthorship W4366605421A5053915349 @default.

• next