Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366769727> ?p ?o ?g. }
- W4366769727 abstract "Abstract Understanding how the atrial and ventricular chambers of the heart maintain their distinct identity is a prerequisite for treating chamber-specific diseases. Here, we selectively inactivated the transcription factor Tbx5 in the atrial working myocardium of the neonatal mouse heart to show that it is required to maintain atrial identity. Atrial Tbx5 inactivation downregulated highly chamber specific genes such as Myl7 and Nppa , and conversely, increased the expression of ventricular identity genes including Myl2 . Using combined single nucleus transcriptome and open chromatin profiling, we assessed genomic accessibility changes underlying the altered atrial identity expression program, identifying 1846 genomic loci with greater accessibility in control atrial cardiomyocytes compared to KO aCMs. 69% of the control-enriched ATAC regions were bound by TBX5, demonstrating a role for TBX5 in maintaining atrial genomic accessibility. These regions were associated with genes that had higher expression in control aCMs compared to KO aCMs, suggesting they act as TBX5-dependent enhancers. We tested this hypothesis by analyzing enhancer chromatin looping using HiChIP and found 510 chromatin loops that were sensitive to TBX5 dosage. Of the loops enriched in control aCMs, 73.7% contained anchors in control-enriched ATAC regions. Together, these data demonstrate a genomic role for TBX5 in maintaining the atrial gene expression program by binding to atrial enhancers and preserving tissue-specific chromatin architecture of atrial enhancers." @default.
- W4366769727 created "2023-04-25" @default.
- W4366769727 creator A5002670202 @default.
- W4366769727 creator A5004151275 @default.
- W4366769727 creator A5005091829 @default.
- W4366769727 creator A5005371628 @default.
- W4366769727 creator A5010376539 @default.
- W4366769727 creator A5011341759 @default.
- W4366769727 creator A5017541072 @default.
- W4366769727 creator A5021544196 @default.
- W4366769727 creator A5029187572 @default.
- W4366769727 creator A5032725801 @default.
- W4366769727 creator A5050110771 @default.
- W4366769727 creator A5052311379 @default.
- W4366769727 creator A5055338544 @default.
- W4366769727 creator A5063742204 @default.
- W4366769727 creator A5070529138 @default.
- W4366769727 creator A5072707319 @default.
- W4366769727 creator A5076662333 @default.
- W4366769727 creator A5082289447 @default.
- W4366769727 creator A5083374356 @default.
- W4366769727 creator A5091051117 @default.
- W4366769727 date "2023-04-22" @default.
- W4366769727 modified "2023-10-04" @default.
- W4366769727 title "<i>Tbx5</i>maintains atrial identity by regulating an atrial enhancer network" @default.
- W4366769727 cites W1973431915 @default.
- W4366769727 cites W1979594866 @default.
- W4366769727 cites W1984883254 @default.
- W4366769727 cites W1998689916 @default.
- W4366769727 cites W2004966039 @default.
- W4366769727 cites W2010655878 @default.
- W4366769727 cites W2011190652 @default.
- W4366769727 cites W2011973215 @default.
- W4366769727 cites W2023538745 @default.
- W4366769727 cites W2027264255 @default.
- W4366769727 cites W2036398635 @default.
- W4366769727 cites W2043059554 @default.
- W4366769727 cites W2047838358 @default.
- W4366769727 cites W2052336762 @default.
- W4366769727 cites W2053348556 @default.
- W4366769727 cites W2070050178 @default.
- W4366769727 cites W2070175168 @default.
- W4366769727 cites W2070857930 @default.
- W4366769727 cites W2086282126 @default.
- W4366769727 cites W2100483634 @default.
- W4366769727 cites W2103441770 @default.
- W4366769727 cites W2103968215 @default.
- W4366769727 cites W2104010513 @default.
- W4366769727 cites W2112387318 @default.
- W4366769727 cites W2112567404 @default.
- W4366769727 cites W2114430445 @default.
- W4366769727 cites W2120328369 @default.
- W4366769727 cites W2123227077 @default.
- W4366769727 cites W2125263315 @default.
- W4366769727 cites W2127388725 @default.
- W4366769727 cites W2128259747 @default.
- W4366769727 cites W2130410032 @default.
- W4366769727 cites W2139456654 @default.
- W4366769727 cites W2152791321 @default.
- W4366769727 cites W2163669025 @default.
- W4366769727 cites W2169456326 @default.
- W4366769727 cites W2172291567 @default.
- W4366769727 cites W2185693210 @default.
- W4366769727 cites W2267370236 @default.
- W4366769727 cites W2481029670 @default.
- W4366769727 cites W2493763501 @default.
- W4366769727 cites W2509059566 @default.
- W4366769727 cites W2523285811 @default.
- W4366769727 cites W2590671119 @default.
- W4366769727 cites W2729948611 @default.
- W4366769727 cites W2742550673 @default.
- W4366769727 cites W2777574440 @default.
- W4366769727 cites W2789833802 @default.
- W4366769727 cites W2794480084 @default.
- W4366769727 cites W2900003149 @default.
- W4366769727 cites W2922997271 @default.
- W4366769727 cites W2949153281 @default.
- W4366769727 cites W2953151710 @default.
- W4366769727 cites W2980316483 @default.
- W4366769727 cites W2997280954 @default.
- W4366769727 cites W3011752560 @default.
- W4366769727 cites W3013105716 @default.
- W4366769727 cites W3034846478 @default.
- W4366769727 cites W3082866380 @default.
- W4366769727 cites W3093565971 @default.
- W4366769727 cites W3093858607 @default.
- W4366769727 cites W3112439613 @default.
- W4366769727 cites W3156113107 @default.
- W4366769727 cites W3164422518 @default.
- W4366769727 cites W3164692211 @default.
- W4366769727 cites W3180162419 @default.
- W4366769727 cites W3195278069 @default.
- W4366769727 cites W3199690223 @default.
- W4366769727 cites W3210860076 @default.
- W4366769727 cites W4224230881 @default.
- W4366769727 cites W4224255802 @default.
- W4366769727 cites W4225572348 @default.
- W4366769727 cites W4226185665 @default.
- W4366769727 cites W4280524211 @default.
- W4366769727 cites W4286203308 @default.