FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366771931> ?p ?o ?g. }

• W4366771931 abstract "Single-cell detection of multiple target analytes is an important goal in cell biology. However, due to the spectral overlap of common fluorophores, multiplexed fluorescence imaging beyond two-to-three targets inside living cells remains a technical challenge. Herein, we introduce a multiplexed imaging strategy that enables live-cell target detection via sequential rounds of imaging-and-stripping process, which is named as sequential Fluorogenic RNA Imaging-Enabled Sensor (seqFRIES). In seqFRIES, multiple orthogonal fluorogenic RNA aptamers are genetically encoded inside cells, and then the corresponding cell membrane permeable dye molecules are added, imaged, and rapidly removed in consecutive detection cycles. As a proof-of-concept, we have identified in this study five in vitro orthogonal fluorogenic RNA aptamer/dye pairs (>10-fold higher fluorescence signals), four of which can be used for highly orthogonal and multiplexed imaging in living bacterial and mammalian cells. After further optimizing the cellular fluorescence activation and deactivation kinetics of these RNA/dye pairs, the whole four-color semi-quantitative seqFRIES process can now be completed in ∼20 min. Meanwhile, seqFRIES-mediated simultaneous detection of two critical signaling molecules, guanosine tetraphosphate and cyclic diguanylate, was also achieved within individual living cells. We expect our validation of this new seqFRIES concept here will facilitate the further development and potential broad usage of these orthogonal fluorogenic RNA/dye pairs for highly multiplexed and dynamic cellular imaging and cell biology studies." @default.
• W4366771931 created "2023-04-25" @default.
• W4366771931 creator A5005394149 @default.
• W4366771931 creator A5010021316 @default.
• W4366771931 creator A5025495733 @default.
• W4366771931 creator A5025531775 @default.
• W4366771931 creator A5029978080 @default.
• W4366771931 creator A5046075766 @default.
• W4366771931 creator A5056442083 @default.
• W4366771931 creator A5076763057 @default.
• W4366771931 creator A5078269576 @default.
• W4366771931 creator A5079073545 @default.
• W4366771931 creator A5082844023 @default.
• W4366771931 creator A5083832032 @default.
• W4366771931 date "2023-04-21" @default.
• W4366771931 modified "2023-10-02" @default.
• W4366771931 title "Multiplexed Sequential Imaging in Living Cells with Orthogonal Fluorogenic RNA Aptamer/Dye Pairs" @default.
• W4366771931 cites W1512470862 @default.
• W4366771931 cites W1600206056 @default.
• W4366771931 cites W1670928643 @default.
• W4366771931 cites W1788709549 @default.
• W4366771931 cites W1817033456 @default.
• W4366771931 cites W1965163468 @default.
• W4366771931 cites W1967652154 @default.
• W4366771931 cites W1976135176 @default.
• W4366771931 cites W1986842298 @default.
• W4366771931 cites W1992182416 @default.
• W4366771931 cites W2004252769 @default.
• W4366771931 cites W2042789810 @default.
• W4366771931 cites W2048055667 @default.
• W4366771931 cites W2073824449 @default.
• W4366771931 cites W2074789242 @default.
• W4366771931 cites W2104525153 @default.
• W4366771931 cites W2114783261 @default.
• W4366771931 cites W2126537932 @default.
• W4366771931 cites W2132665880 @default.
• W4366771931 cites W2137232836 @default.
• W4366771931 cites W2148315081 @default.
• W4366771931 cites W2152256166 @default.
• W4366771931 cites W2332645767 @default.
• W4366771931 cites W2586995851 @default.
• W4366771931 cites W2605479282 @default.
• W4366771931 cites W2617667835 @default.
• W4366771931 cites W2760454171 @default.
• W4366771931 cites W2776613732 @default.
• W4366771931 cites W2803572544 @default.
• W4366771931 cites W2886822966 @default.
• W4366771931 cites W2890458481 @default.
• W4366771931 cites W2902010092 @default.
• W4366771931 cites W2912366427 @default.
• W4366771931 cites W2923300855 @default.
• W4366771931 cites W2935696812 @default.
• W4366771931 cites W2936259264 @default.
• W4366771931 cites W2973830670 @default.
• W4366771931 cites W2980295181 @default.
• W4366771931 cites W2980692434 @default.
• W4366771931 cites W2981059853 @default.
• W4366771931 cites W3003369484 @default.
• W4366771931 cites W3008734037 @default.
• W4366771931 cites W3040326917 @default.
• W4366771931 cites W3101640959 @default.
• W4366771931 cites W3177310535 @default.
• W4366771931 cites W3196582334 @default.
• W4366771931 cites W4228997517 @default.
• W4366771931 cites W4281838146 @default.
• W4366771931 doi "https://doi.org/10.1101/2023.04.20.537750" @default.
• W4366771931 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37131625" @default.
• W4366771931 hasPublicationYear "2023" @default.
• W4366771931 type Work @default.
• W4366771931 citedByCount "1" @default.
• W4366771931 countsByYear W43667719312023 @default.
• W4366771931 crossrefType "posted-content" @default.
• W4366771931 hasAuthorship W4366771931A5005394149 @default.
• W4366771931 hasAuthorship W4366771931A5010021316 @default.
• W4366771931 hasAuthorship W4366771931A5025495733 @default.
• W4366771931 hasAuthorship W4366771931A5025531775 @default.
• W4366771931 hasAuthorship W4366771931A5029978080 @default.
• W4366771931 hasAuthorship W4366771931A5046075766 @default.
• W4366771931 hasAuthorship W4366771931A5056442083 @default.
• W4366771931 hasAuthorship W4366771931A5076763057 @default.
• W4366771931 hasAuthorship W4366771931A5078269576 @default.
• W4366771931 hasAuthorship W4366771931A5079073545 @default.
• W4366771931 hasAuthorship W4366771931A5082844023 @default.
• W4366771931 hasAuthorship W4366771931A5083832032 @default.
• W4366771931 hasBestOaLocation W43667719311 @default.
• W4366771931 hasConcept C10390740 @default.
• W4366771931 hasConcept C104317684 @default.
• W4366771931 hasConcept C121332964 @default.
• W4366771931 hasConcept C12554922 @default.
• W4366771931 hasConcept C128972844 @default.
• W4366771931 hasConcept C1491633281 @default.
• W4366771931 hasConcept C153911025 @default.
• W4366771931 hasConcept C15526883 @default.
• W4366771931 hasConcept C185592680 @default.
• W4366771931 hasConcept C19275194 @default.
• W4366771931 hasConcept C41008148 @default.
• W4366771931 hasConcept C43617362 @default.
• W4366771931 hasConcept C55493867 @default.
• W4366771931 hasConcept C57477423 @default.
• W4366771931 hasConcept C62520636 @default.

• next