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- W4366815119 abstract "Visual processing in the retina depends on the collective activity of large ensembles of neurons organized in different layers. Current techniques for measuring activity of layer-specific neural ensembles rely on expensive pulsed infrared lasers to drive 2-photon activation of calcium-dependent fluorescent reporters. We present a 1-photon light-sheet imaging system that can measure the activity in hundreds of neurons in the ex vivo retina over a large field of view while presenting visual stimuli. This allows for a reliable functional classification of different retinal cell types. We also demonstrate that the system has sufficient resolution to image calcium entry at individual synaptic release sites across the axon terminals of dozens of simultaneously imaged bipolar cells. The simple design, large field of view, and fast image acquisition make this a powerful system for high-throughput and high-resolution measurements of retinal processing at a fraction of the cost of alternative approaches." @default.
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- W4366815119 date "2023-04-01" @default.
- W4366815119 modified "2023-10-14" @default.
- W4366815119 title "Large-scale interrogation of retinal cell functions by 1-photon light-sheet microscopy" @default.
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- W4366815119 doi "https://doi.org/10.1016/j.crmeth.2023.100453" @default.
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