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• W4366816589 abstract "RNA-protein interactions are key to many aspects of cellular homeostasis and their identification is important to understanding cellular function. Multiple strategies have been developed for the RNA-centric characterization of RNA-protein complexes. However, these studies have all been done in immortalized cell lines that do not capture the complexity of heterogeneous tissue samples. Here, we develop hybridization purification of RNA-protein complexes followed by mass spectrometry (HyPR-MS) for use in tissue samples. We isolated both polyadenylated RNA and the specific long noncoding RNA MALAT1 and characterized their protein interactomes. These results demonstrate the feasibility of HyPR-MS in tissue for the multiplexed characterization of specific RNA-protein complexes." @default.
• W4366816589 created "2023-04-25" @default.
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• W4366816589 date "2023-04-24" @default.
• W4366816589 modified "2023-10-16" @default.
• W4366816589 title "Elucidating the RNA–Protein Interactomes of Target RNAs in Tissue" @default.
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• W4366816589 doi "https://doi.org/10.1021/acs.analchem.2c05635" @default.
• W4366816589 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37093976" @default.
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