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- W4366819360 abstract "In this paper, simple imine-based organic fluorophore 4-amino-3-(anthracene-9 yl methyleneamino) phenyl (phenyl) methanone (APM) has been synthesized via a greener approach and the same was used to construct a fluorescent immunoassay for the detection of Listeria monocytogenes (LM). A monoclonal antibody of LM was tagged with APM via the conjugation of the amine group in APM and the acid group of anti-LM through EDC/NHS coupling. The designed immunoassay was optimized for the specific detection of LM in the presence of other interfering pathogens based on the aggregation-induced emission mechanism and the formation of aggregates and their morphology was confirmed with the help of scanning electron microscopy. Density functional theory studies were done to further support the sensing mechanism-based changes in the energy level distribution. All photophysical parameters were measured by using fluorescence spectroscopy techniques. Specific and competitive recognition of LM was done in the presence of other relevant pathogens. The immunoassay shows a linear appreciable range from 1.6 × 106-2.7024 × 108 cfu/mL using the standard plate count method. The LOD has been calculated from the linear equation and the value is found as 3.2 cfu/mL, and this is the lowest LOD value reported for the detection of LM so far. The practical applications of the immunoassay were demonstrated in various food samples, and their accuracy obtained was highly comparable with the standard existing ELISA method." @default.
- W4366819360 created "2023-04-25" @default.
- W4366819360 creator A5017899780 @default.
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- W4366819360 date "2023-04-24" @default.
- W4366819360 modified "2023-10-14" @default.
- W4366819360 title "Optical Immunosensor for the Detection of <i>Listeria monocytogenes</i> in Food Matrixes" @default.
- W4366819360 cites W1597031200 @default.
- W4366819360 cites W1674147318 @default.
- W4366819360 cites W1736731718 @default.
- W4366819360 cites W1841386883 @default.
- W4366819360 cites W1904022102 @default.
- W4366819360 cites W1963644338 @default.
- W4366819360 cites W1963907519 @default.
- W4366819360 cites W1965042557 @default.
- W4366819360 cites W1968187330 @default.
- W4366819360 cites W1980694079 @default.
- W4366819360 cites W1987272549 @default.
- W4366819360 cites W1990271344 @default.
- W4366819360 cites W1997176679 @default.
- W4366819360 cites W1999210935 @default.
- W4366819360 cites W2004167046 @default.
- W4366819360 cites W2016172749 @default.
- W4366819360 cites W2017012092 @default.
- W4366819360 cites W2018539647 @default.
- W4366819360 cites W2023271753 @default.
- W4366819360 cites W2024793618 @default.
- W4366819360 cites W2027060308 @default.
- W4366819360 cites W2039607756 @default.
- W4366819360 cites W2041390593 @default.
- W4366819360 cites W2047909468 @default.
- W4366819360 cites W2048651370 @default.
- W4366819360 cites W2048736135 @default.
- W4366819360 cites W2057616332 @default.
- W4366819360 cites W2058101641 @default.
- W4366819360 cites W2058363175 @default.
- W4366819360 cites W2062253375 @default.
- W4366819360 cites W2075330115 @default.
- W4366819360 cites W2079507386 @default.
- W4366819360 cites W2092156539 @default.
- W4366819360 cites W2093045455 @default.
- W4366819360 cites W2101473165 @default.
- W4366819360 cites W2118910851 @default.
- W4366819360 cites W2120630118 @default.
- W4366819360 cites W2138983601 @default.
- W4366819360 cites W2141126994 @default.
- W4366819360 cites W2143981217 @default.
- W4366819360 cites W2156824859 @default.
- W4366819360 cites W2163639044 @default.
- W4366819360 cites W2170141161 @default.
- W4366819360 cites W2171182365 @default.
- W4366819360 cites W2201255477 @default.
- W4366819360 cites W2233213627 @default.
- W4366819360 cites W2324263672 @default.
- W4366819360 cites W2345574335 @default.
- W4366819360 cites W2409428719 @default.
- W4366819360 cites W2462404684 @default.
- W4366819360 cites W2472144529 @default.
- W4366819360 cites W2560679651 @default.
- W4366819360 cites W2564762764 @default.
- W4366819360 cites W2571116994 @default.
- W4366819360 cites W2572861445 @default.
- W4366819360 cites W2585936553 @default.
- W4366819360 cites W2590156219 @default.
- W4366819360 cites W2591867446 @default.
- W4366819360 cites W2606359489 @default.
- W4366819360 cites W2669771208 @default.
- W4366819360 cites W2759911647 @default.
- W4366819360 cites W2803292899 @default.
- W4366819360 cites W2805268276 @default.
- W4366819360 cites W2898351467 @default.
- W4366819360 cites W2909350202 @default.
- W4366819360 cites W2912709072 @default.
- W4366819360 cites W2920682198 @default.
- W4366819360 cites W2928553707 @default.
- W4366819360 cites W2943263871 @default.
- W4366819360 cites W2945624233 @default.
- W4366819360 cites W2948070903 @default.
- W4366819360 cites W2964189826 @default.
- W4366819360 cites W2965761395 @default.
- W4366819360 cites W2978742357 @default.
- W4366819360 cites W2991065675 @default.
- W4366819360 cites W2999328467 @default.
- W4366819360 cites W3013522775 @default.
- W4366819360 cites W3109156361 @default.
- W4366819360 cites W3112960276 @default.
- W4366819360 cites W3187138206 @default.
- W4366819360 cites W3207819975 @default.
- W4366819360 cites W4214610912 @default.
- W4366819360 cites W4239350813 @default.
- W4366819360 cites W4244248235 @default.
- W4366819360 doi "https://doi.org/10.1021/acsomega.2c07848" @default.
- W4366819360 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37179640" @default.