FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366831460> ?p ?o ?g. }

• W4366831460 endingPage "282" @default.
• W4366831460 startingPage "273" @default.
• W4366831460 abstract "Saccharomyces cerevisiae is a robust cell factory to secrete or surface-display cellulase and amylase for the conversion of agricultural residues into valuable chemicals. Engineering the secretory pathway is a well-known strategy for overproducing these enzymes. Although cell wall biosynthesis can be tightly linked to the secretory pathway by regulation of all involved processes, the effect of its modifications on protein production has not been extensively studied. In this study, we systematically studied the effect of engineering cell wall biosynthesis on the activity of cellulolytic enzyme β-glucosidase (BGL1) by comparing seventy-nine gene knockout S. cerevisiae strains and newly identified that inactivation of DFG5, YPK1, FYV5, CCW12 and KRE1 obviously improved BGL1 secretion and surface-display. Combinatorial modifications of these genes, particularly double deletion of FVY5 and CCW12, along with the use of rich medium, increased the activity of secreted and surface-displayed BGL1 by 6.13-fold and 7.99-fold, respectively. Additionally, we applied this strategy to improve the activity of the cellulolytic cellobiohydrolase and amylolytic α-amylase. Through proteomic analysis coupled with reverse engineering, we found that in addition to the secretory pathway, regulation of translation processes may also involve in improving enzyme activity by engineering cell wall biosynthesis. Our work provides new insight into the construction of a yeast cell factory for efficient production of polysaccharide degrading enzymes." @default.
• W4366831460 created "2023-04-25" @default.
• W4366831460 creator A5005377453 @default.
• W4366831460 creator A5011051211 @default.
• W4366831460 creator A5012331413 @default.
• W4366831460 creator A5026594174 @default.
• W4366831460 creator A5033466926 @default.
• W4366831460 creator A5038787011 @default.
• W4366831460 creator A5080577104 @default.
• W4366831460 creator A5082143549 @default.
• W4366831460 creator A5084323384 @default.
• W4366831460 date "2023-05-01" @default.
• W4366831460 modified "2023-10-01" @default.
• W4366831460 title "Systematic genetic modifications of cell wall biosynthesis enhanced the secretion and surface-display of polysaccharide degrading enzymes in Saccharomyces cerevisiae" @default.
• W4366831460 cites W1904777810 @default.
• W4366831460 cites W1978429934 @default.
• W4366831460 cites W1978877135 @default.
• W4366831460 cites W1987358333 @default.
• W4366831460 cites W2011425644 @default.
• W4366831460 cites W2011470071 @default.
• W4366831460 cites W2013541506 @default.
• W4366831460 cites W2014122943 @default.
• W4366831460 cites W2033896965 @default.
• W4366831460 cites W2035618305 @default.
• W4366831460 cites W2038805308 @default.
• W4366831460 cites W2043456995 @default.
• W4366831460 cites W2045699047 @default.
• W4366831460 cites W2053195919 @default.
• W4366831460 cites W2053260983 @default.
• W4366831460 cites W2054851403 @default.
• W4366831460 cites W2059025873 @default.
• W4366831460 cites W2071046609 @default.
• W4366831460 cites W2078988344 @default.
• W4366831460 cites W2090849724 @default.
• W4366831460 cites W2099636105 @default.
• W4366831460 cites W2106188607 @default.
• W4366831460 cites W2110971717 @default.
• W4366831460 cites W2111311345 @default.
• W4366831460 cites W2121169956 @default.
• W4366831460 cites W2130494754 @default.
• W4366831460 cites W2150699248 @default.
• W4366831460 cites W2151244582 @default.
• W4366831460 cites W2157937554 @default.
• W4366831460 cites W2159139667 @default.
• W4366831460 cites W2167570426 @default.
• W4366831460 cites W2312226947 @default.
• W4366831460 cites W2396809607 @default.
• W4366831460 cites W2408236302 @default.
• W4366831460 cites W2549682468 @default.
• W4366831460 cites W2559716871 @default.
• W4366831460 cites W2594240134 @default.
• W4366831460 cites W2611287520 @default.
• W4366831460 cites W2765512061 @default.
• W4366831460 cites W2899734176 @default.
• W4366831460 cites W2947876672 @default.
• W4366831460 cites W2952923206 @default.
• W4366831460 cites W2973457491 @default.
• W4366831460 cites W3006134865 @default.
• W4366831460 cites W3037744967 @default.
• W4366831460 cites W3104537418 @default.
• W4366831460 cites W4200014812 @default.
• W4366831460 cites W4211120708 @default.
• W4366831460 cites W4223622447 @default.
• W4366831460 cites W4281774287 @default.
• W4366831460 cites W4295412555 @default.
• W4366831460 cites W4306180742 @default.
• W4366831460 cites W4306320806 @default.
• W4366831460 cites W4308421447 @default.
• W4366831460 doi "https://doi.org/10.1016/j.ymben.2023.04.011" @default.
• W4366831460 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37100192" @default.
• W4366831460 hasPublicationYear "2023" @default.
• W4366831460 type Work @default.
• W4366831460 citedByCount "2" @default.
• W4366831460 countsByYear W43668314602023 @default.
• W4366831460 crossrefType "journal-article" @default.
• W4366831460 hasAuthorship W4366831460A5005377453 @default.
• W4366831460 hasAuthorship W4366831460A5011051211 @default.
• W4366831460 hasAuthorship W4366831460A5012331413 @default.
• W4366831460 hasAuthorship W4366831460A5026594174 @default.
• W4366831460 hasAuthorship W4366831460A5033466926 @default.
• W4366831460 hasAuthorship W4366831460A5038787011 @default.
• W4366831460 hasAuthorship W4366831460A5080577104 @default.
• W4366831460 hasAuthorship W4366831460A5082143549 @default.
• W4366831460 hasAuthorship W4366831460A5084323384 @default.
• W4366831460 hasConcept C119062480 @default.
• W4366831460 hasConcept C125235067 @default.
• W4366831460 hasConcept C1491633281 @default.
• W4366831460 hasConcept C181199279 @default.
• W4366831460 hasConcept C2777576037 @default.
• W4366831460 hasConcept C2779222958 @default.
• W4366831460 hasConcept C49039625 @default.
• W4366831460 hasConcept C553450214 @default.
• W4366831460 hasConcept C55493867 @default.
• W4366831460 hasConcept C6350086 @default.
• W4366831460 hasConcept C6492254 @default.
• W4366831460 hasConcept C86803240 @default.
• W4366831460 hasConceptScore W4366831460C119062480 @default.
• W4366831460 hasConceptScore W4366831460C125235067 @default.

• next