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- W4366979969 abstract "<i>Escherichia coli</i> and <i>Salmonella enterica</i> are significant causes of gastrointestinal disease globally. The Contamination Sanitization Inspection and Disinfection (CSI-D) device is a handheld fluorescence-based imaging system that disinfects food contact surfaces using ultraviolet-C (UVC) illumination. The goal of this study was to determine the optimal parameters for disinfection of E. coli and S. enterica using the CSI-D system. <i>E. coli</i> and <i>S. enterica</i> Enteritidis, Newport, Typhimurium, and Javiana were grown on selective media, followed by transfer to Luria Bertani broth. After overnight incubation, the cultures were diluted and spread-plated on L-agar. The plates were exposed to high-intensity (10 mW/cm2) or low-intensity (5 mW/cm2) UVC for 1 s, 3 s, or 5 s. Exposed and control plates were incubated at room temperature for 2-3 h, then overnight at 37°C. The resulting colonies were counted and compared to control plates. Three trials were conducted on separate days. The average of the trials showed that exposure times of 3-5 s at either intensity resulted in effective and consistent destruction of E. coli and S. enterica. The minimum reduction at 3 s exposure for both intensities was 96%, with a maximum of 100%. The 1 s exposure time showed inconsistent results, with a 0-61.5% survival rate. The results of this study show that exposure to UVC for at least 3 s is required to achieve consistent disinfection of 96-100% for generic <i>E. coli</i> and <i>S. enterica</i>." @default.
- W4366979969 created "2023-04-27" @default.
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- W4366979969 date "2023-06-13" @default.
- W4366979969 modified "2023-10-12" @default.
- W4366979969 title "Disinfection of Escherichia coli and Salmonella enterica using the Contamination Sanitization Inspection and Disinfection (CSI-D) system" @default.
- W4366979969 doi "https://doi.org/10.1117/12.2664049" @default.
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