FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366985995> ?p ?o ?g. }

• W4366985995 abstract "Abstract Human mesenchymal stem cells (hMSCs) are multipotent cells that can differentiate into adipocytes, chondrocytes and osteoblasts. Due to their differentiation potential, hMSCs are among the most frequently used cells for therapeutic applications in tissue engineering and regenerative medicine. However, the number of cells obtained through isolation alone is insufficient for hMSC-based therapies and basic research, necessitating their in-vitro expansion. Conventionally, this is often carried out on rigid surfaces such as tissue culture petriplates (TCPs). However, during in-vitro expansion, hMSCs lose their proliferative ability and multilineage differentiation potential, making them unsuitable for clinical use. Although multiple approaches have been tried to maintain hMSC stemness over prolonged expansion, finding a suitable culture system to achieve this remains an unmet need. Recently, few research groups including ours have shown that hMSCs maintain their stemness over long passages when cultured on soft substrate. In addition, it has been shown that hMSCs cultured on soft substrates have more condensed chromatin and lower levels of histone acetylation compared to those cultured on stiff substrates. It has also been shown that condensing/decondensing chromatin by deacetylation/acetylation can delay/hasten replicative senescence in hMSCs during long-term expansion on TCPs. However, how chromatin condensation/decondensation influences nuclear morphology and DNA damage - which are strongly related to the onset of senescence and cancer - is still not known. To answer this question, here we cultured hMSCs for long duration (P4-P11) in presence of epigenetic modifiers histone acetyltransferase inhibitor (HATi) which promotes chromatin condensation by preventing histone acetylation and histone deacetylase inhibitor (HDACi) which promotes chromatin decondensation and investigated their effect on various nuclear markers related to senescence and cancer. We have found that consistent acetylation causes severe nuclear abnormalities whereas chromatin condensation by deacetylation helps in safeguarding nucleus from damages caused by in-vitro expansion." @default.
• W4366985995 created "2023-04-27" @default.
• W4366985995 creator A5014905005 @default.
• W4366985995 creator A5017211248 @default.
• W4366985995 creator A5039517027 @default.
• W4366985995 creator A5043547092 @default.
• W4366985995 creator A5052503810 @default.
• W4366985995 date "2023-04-25" @default.
• W4366985995 modified "2023-09-29" @default.
• W4366985995 title "Chromatin condensation delays human mesenchymal stem cells senescence by safeguarding nuclear damages during long term<i>in vitro</i>expansion" @default.
• W4366985995 cites W1521707994 @default.
• W4366985995 cites W1600024584 @default.
• W4366985995 cites W1804670053 @default.
• W4366985995 cites W1978468353 @default.
• W4366985995 cites W2002661570 @default.
• W4366985995 cites W2012222058 @default.
• W4366985995 cites W2079983476 @default.
• W4366985995 cites W2095048053 @default.
• W4366985995 cites W2109127788 @default.
• W4366985995 cites W2140115841 @default.
• W4366985995 cites W2221267861 @default.
• W4366985995 cites W2329076138 @default.
• W4366985995 cites W2494515430 @default.
• W4366985995 cites W2517275410 @default.
• W4366985995 cites W2547269836 @default.
• W4366985995 cites W2628954376 @default.
• W4366985995 cites W2759661759 @default.
• W4366985995 cites W2765510117 @default.
• W4366985995 cites W2769613435 @default.
• W4366985995 cites W2887443702 @default.
• W4366985995 cites W2892182392 @default.
• W4366985995 cites W2892331902 @default.
• W4366985995 cites W2904858662 @default.
• W4366985995 cites W2935637356 @default.
• W4366985995 cites W2942131830 @default.
• W4366985995 cites W3128059144 @default.
• W4366985995 cites W3144561945 @default.
• W4366985995 cites W3158830853 @default.
• W4366985995 cites W4205487118 @default.
• W4366985995 cites W4213310651 @default.
• W4366985995 cites W4244784488 @default.
• W4366985995 cites W4291793058 @default.
• W4366985995 doi "https://doi.org/10.1101/2023.04.22.537784" @default.
• W4366985995 hasPublicationYear "2023" @default.
• W4366985995 type Work @default.
• W4366985995 citedByCount "0" @default.
• W4366985995 crossrefType "posted-content" @default.
• W4366985995 hasAuthorship W4366985995A5014905005 @default.
• W4366985995 hasAuthorship W4366985995A5017211248 @default.
• W4366985995 hasAuthorship W4366985995A5039517027 @default.
• W4366985995 hasAuthorship W4366985995A5043547092 @default.
• W4366985995 hasAuthorship W4366985995A5052503810 @default.
• W4366985995 hasBestOaLocation W43669859951 @default.
• W4366985995 hasConcept C104317684 @default.
• W4366985995 hasConcept C119157956 @default.
• W4366985995 hasConcept C185592680 @default.
• W4366985995 hasConcept C198826908 @default.
• W4366985995 hasConcept C41091548 @default.
• W4366985995 hasConcept C502942594 @default.
• W4366985995 hasConcept C522857546 @default.
• W4366985995 hasConcept C54355233 @default.
• W4366985995 hasConcept C552990157 @default.
• W4366985995 hasConcept C64927066 @default.
• W4366985995 hasConcept C83640560 @default.
• W4366985995 hasConcept C86803240 @default.
• W4366985995 hasConcept C95444343 @default.
• W4366985995 hasConceptScore W4366985995C104317684 @default.
• W4366985995 hasConceptScore W4366985995C119157956 @default.
• W4366985995 hasConceptScore W4366985995C185592680 @default.
• W4366985995 hasConceptScore W4366985995C198826908 @default.
• W4366985995 hasConceptScore W4366985995C41091548 @default.
• W4366985995 hasConceptScore W4366985995C502942594 @default.
• W4366985995 hasConceptScore W4366985995C522857546 @default.
• W4366985995 hasConceptScore W4366985995C54355233 @default.
• W4366985995 hasConceptScore W4366985995C552990157 @default.
• W4366985995 hasConceptScore W4366985995C64927066 @default.
• W4366985995 hasConceptScore W4366985995C83640560 @default.
• W4366985995 hasConceptScore W4366985995C86803240 @default.
• W4366985995 hasConceptScore W4366985995C95444343 @default.
• W4366985995 hasLocation W43669859951 @default.
• W4366985995 hasOpenAccess W4366985995 @default.
• W4366985995 hasPrimaryLocation W43669859951 @default.
• W4366985995 hasRelatedWork W11940275 @default.
• W4366985995 hasRelatedWork W2056537646 @default.
• W4366985995 hasRelatedWork W2060997207 @default.
• W4366985995 hasRelatedWork W2103827219 @default.
• W4366985995 hasRelatedWork W2140331030 @default.
• W4366985995 hasRelatedWork W2254854198 @default.
• W4366985995 hasRelatedWork W2405260757 @default.
• W4366985995 hasRelatedWork W2540150361 @default.
• W4366985995 hasRelatedWork W283994129 @default.
• W4366985995 hasRelatedWork W4213310651 @default.
• W4366985995 isParatext "false" @default.
• W4366985995 isRetracted "false" @default.
• W4366985995 workType "article" @default.