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- W4366988345 abstract "Abstract Aim Candida auris, fast evolving drug-resistant fungus, poses an imminent global health threat. Alternative drug-resistance nonevoking treatment options are necessary. This study explored the antifungal and antibiofilm efficacies of Withania somnifera seed oil extracted using super critical CO2 (WSSO) against clinically isolated Fluconazole-resistant C. auris and its putative mode-of-action. Methods and Results Effects of WSSO on C. auris were tested by broth microdilution method, with observed IC50 at 5.96 mg ml−1. Time-kill assay revealed that WSSO is fungistatic. Mechanistically, ergosterol binding and sorbitol protection assays showed that C. auris cell membrane and cell wall are the targets for WSSO. Lactophenol: Cotton-Blue: Trypan-Blue staining confirmed loss of intracellular contents by WSSO treatment. Candida auris biofilm formation was disrupted by WSSO (BIC50: 8.52 mg ml−1). Additionally, WSSO exhibited dose and time-dependent mature biofilm eradication property with 50% efficacies at 23.27, 19.28, 18.18, and 7.22 mg ml−1 over 24, 48, 72, and 96 h, respectively. Biofilm eradication by WSSO was further substantiated through scanning electron microscopy. Standard-of-Care Amphotericin B, at its break-point concentration, (2 μg ml−1) was found to be inefficient as an antibiofilm agent. Conclusions WSSO is a potent antifungal agent effective against planktonic C. auris and its biofilm." @default.
- W4366988345 created "2023-04-27" @default.
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- W4366988345 date "2023-04-24" @default.
- W4366988345 modified "2023-09-26" @default.
- W4366988345 title "<i>Withania somnifera</i> Seed Oil Exhibits Anti-biofilm Properties Against Drug-resistant <i>Candida auris</i> Clinical Isolate through Modulation in Cell Permeability" @default.
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- W4366988345 doi "https://doi.org/10.1093/jambio/lxad087" @default.
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