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• W4366993055 abstract "Osteoarticular infections (OAI) are a significant cause of morbidity and mortality. Cultures and serology are some of the gold standards for identifying infection but are often unable to provide a timely diagnosis or a diagnosis at all. Genetic testing offers capabilities that other modalities lack. Polymerase chain reaction has multiple versions with various costs and turnaround times. This technology has become implemented in multiple pediatric center OAI diagnostic protocols. There is sufficient literature documenting effectiveness in certain clinical situations, especially with fastidious organism diagnosis, but significant limitation still exists. Metagenomic next-generation sequencing is an unbiased or hypothesis-free modality with the capability to detect the genetic material of bacteria, viruses, parasites, fungi, and humans from a single sample. Potential benefits include pathogen identification unaffected by antimicrobial administration, detection of fastidious organisms more quickly, delineation of pathogens in polymicrobial infections, antimicrobial susceptibility, and avoidance of invasive procedures. It is a resource-intensive modality with little standardization of the complex processes. Appropriate use and definitive clinical impact have yet to be determined." @default.
• W4366993055 created "2023-04-27" @default.
• W4366993055 creator A5005572045 @default.
• W4366993055 creator A5012636781 @default.
• W4366993055 date "2023-04-01" @default.
• W4366993055 modified "2023-10-14" @default.
• W4366993055 title "Metagenomic next-generation sequencing in osteoarticular infections with a focus on pediatrics: current concepts and clinical applications" @default.
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