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- W4366996556 abstract "To explore the effects of Ena/VASP gene family on the expression of glycoprotein (GP) Ib-IX complex in human megakaryoblastic leukemia Dami cells.SiRNAs targeting Ena/VASP gene family were designed and synthesized to interfere Enah, EVL and VASP gene expression. When the siRNAs were transfected into Dami cells by using LipofectamineTM 2000 for 48 h, the expression of GPIb-IX complex was detected by quantitative real-time PCR, Western blot and flow cytometry.We successfully established siVASP , siEVL and si Enah Dami cell lines. And it was found that the expression of GPIb-IX complex had no evident reduction in siEVL or siVASP Dami cells at both mRNA and protein level, while the total protein and membrane protein of GPIb-IX complex were obviously reduced when Enah was knocked down.Enah could affect the expression of GPIb-IX complex in human megakaryoblastic leukemia Dami cells, but the underlying mechanism still needs to be further explored.Ena/VASP 家族对人巨核细胞白血病Dami细胞中GPIb-IX复合物表达的影响.探索Ena/VASP 家族对人巨核细胞白血病Dami细胞中GPIb-IX复合物表达的影响。.设计合成针对Ena/VASP 家族的 Enah、EVL 及VASP 3个基因的干扰siRNA，通过Lipofectamine TM 2000转染人巨核细胞白血病Dami细胞株，培养48 h后收取细胞，利用qRT-PCR、Western blot和流式细胞术等方法检测巨核细胞中GPIb-IX复合物的表达情况。.构建了Ena/VASP 家族基因干扰效果较佳的Dami细胞株。在敲减 Enah、EVL 及VASP 基因表 达后，在mRNA水平上，巨核细胞中GPIb-IX复合物的表达无明显变化；在总蛋白和膜表面蛋白水平上，敲减 Enah后，巨核细胞中GPIb-IX复合物表达明显降低。.Enah基因可能对人巨核细胞白血病Dami细胞中GPIb-IX复合物的表达有影响，但是具体机制仍需要进一步探索。." @default.
- W4366996556 created "2023-04-27" @default.
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- W4366996556 date "2023-04-01" @default.
- W4366996556 modified "2023-10-18" @default.
- W4366996556 title "[The Effect of Ena/VASP Family on the Expression of GPIb-IX Complex in Human Megakaryoblastic Leukemia Dami Cells]." @default.
- W4366996556 doi "https://doi.org/10.19746/j.cnki.issn.1009-2137.2023.02.025" @default.
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