Matches in SemOpenAlex for { <https://semopenalex.org/work/W4366996559> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W4366996559 endingPage "502" @default.
- W4366996559 startingPage "495" @default.
- W4366996559 abstract "To study the effect of gradient shear stress on platelet aggregation by microfluidic chip Technology.Microfluidic chip was used to simulate 80% fixed stenotic microchannel, and the hydrodynamic behavior of the stenotic microchannel model was analyzed by the finite element analysis module of sollidwork software. Microfluidic chip was used to analyze the adhesion and aggregation behavior of platelets in patients with different diseases, and flow cytometry was used to detect expression of the platelet activation marker CD62p. Aspirin, Tirofiban and protocatechuic acid were used to treat the blood, and the adhesion and aggregation of platelets were observed by fluorescence microscope.The gradient fluid shear rate produced by the stenosis model of microfluidic chip could induce platelet aggregation, and the degree of platelet adhesion and aggregation increased with the increase of shear rate within a certain range of shear rate. The effect of platelet aggregation in patients with arterial thrombotic diseases were significantly higher than normal group (P<0.05), and the effect of platelet aggregation in patients with myelodysplastic disease was lower than normal group (P<0.05).The microfluidic chip analysis technology can accurately analyze and evaluate the platelet adhesion and aggregation effects of various thrombotic diseases unde the environment of the shear rate, and is helpful for auxiliary diagnosis of clinical thrombotic diseases.微流控芯片技术研究梯度变化的剪切力诱导的血小板粘附、聚集效应.应用微流控芯片分析技术研究梯度变化的剪切力对血小板聚集的影响.应用微流控芯片模拟80%固定狭窄微通道，微通道狭窄模型利用三维建模软件sollidwork自带的有限元分析模块进行流体动力学行为分析。应用微流控芯片分析不同疾病患者血液的血小板粘附和聚集行为，以及采用流式细胞术检测血小板活化标志物CD62p的表达。同时使用抗血小板药物阿司匹林、替罗非班、原儿茶酸、CD42b处理血液，并通过荧光显微镜实时观察血小板粘附和聚集行为.微流控芯片狭窄模型产生的梯度变化的流体剪切率能够诱导血小板聚集，且在一定剪切率范围内血小板粘附聚集程度随着剪切率的增加而增加。动脉血栓性疾病患者的血小板聚集效应明显高于正常对照组(P<0.05)，骨髓增生异常患者血小板聚集效应低于正常对照组(P<0.05).在剪切力存在环境下微流控芯片分析技术能够准确分析评估各种血栓性疾病的血小板黏附聚集效应，有助于临床血栓类疾病的辅助诊断." @default.
- W4366996559 created "2023-04-27" @default.
- W4366996559 creator A5008008825 @default.
- W4366996559 creator A5046109113 @default.
- W4366996559 creator A5055021699 @default.
- W4366996559 creator A5074573104 @default.
- W4366996559 creator A5078387367 @default.
- W4366996559 creator A5082279294 @default.
- W4366996559 date "2023-04-01" @default.
- W4366996559 modified "2023-09-27" @default.
- W4366996559 title "[Study on Platelet Adhesion and Aggregation Induced by Gradient Shear Stress Using Microfluidic Chip Technology]." @default.
- W4366996559 doi "https://doi.org/10.19746/j.cnki.issn.1009-2137.2023.02.027" @default.
- W4366996559 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37096525" @default.
- W4366996559 hasPublicationYear "2023" @default.
- W4366996559 type Work @default.
- W4366996559 citedByCount "0" @default.
- W4366996559 crossrefType "journal-article" @default.
- W4366996559 hasAuthorship W4366996559A5008008825 @default.
- W4366996559 hasAuthorship W4366996559A5046109113 @default.
- W4366996559 hasAuthorship W4366996559A5055021699 @default.
- W4366996559 hasAuthorship W4366996559A5074573104 @default.
- W4366996559 hasAuthorship W4366996559A5078387367 @default.
- W4366996559 hasAuthorship W4366996559A5082279294 @default.
- W4366996559 hasConcept C126322002 @default.
- W4366996559 hasConcept C127172972 @default.
- W4366996559 hasConcept C136229726 @default.
- W4366996559 hasConcept C159985019 @default.
- W4366996559 hasConcept C165682214 @default.
- W4366996559 hasConcept C171250308 @default.
- W4366996559 hasConcept C185592680 @default.
- W4366996559 hasConcept C192562407 @default.
- W4366996559 hasConcept C21141959 @default.
- W4366996559 hasConcept C2988198817 @default.
- W4366996559 hasConcept C3018697912 @default.
- W4366996559 hasConcept C71924100 @default.
- W4366996559 hasConcept C84416704 @default.
- W4366996559 hasConcept C8673954 @default.
- W4366996559 hasConcept C89560881 @default.
- W4366996559 hasConceptScore W4366996559C126322002 @default.
- W4366996559 hasConceptScore W4366996559C127172972 @default.
- W4366996559 hasConceptScore W4366996559C136229726 @default.
- W4366996559 hasConceptScore W4366996559C159985019 @default.
- W4366996559 hasConceptScore W4366996559C165682214 @default.
- W4366996559 hasConceptScore W4366996559C171250308 @default.
- W4366996559 hasConceptScore W4366996559C185592680 @default.
- W4366996559 hasConceptScore W4366996559C192562407 @default.
- W4366996559 hasConceptScore W4366996559C21141959 @default.
- W4366996559 hasConceptScore W4366996559C2988198817 @default.
- W4366996559 hasConceptScore W4366996559C3018697912 @default.
- W4366996559 hasConceptScore W4366996559C71924100 @default.
- W4366996559 hasConceptScore W4366996559C84416704 @default.
- W4366996559 hasConceptScore W4366996559C8673954 @default.
- W4366996559 hasConceptScore W4366996559C89560881 @default.
- W4366996559 hasIssue "2" @default.
- W4366996559 hasLocation W43669965591 @default.
- W4366996559 hasOpenAccess W4366996559 @default.
- W4366996559 hasPrimaryLocation W43669965591 @default.
- W4366996559 hasRelatedWork W1768333654 @default.
- W4366996559 hasRelatedWork W1881826640 @default.
- W4366996559 hasRelatedWork W2077883450 @default.
- W4366996559 hasRelatedWork W2129617136 @default.
- W4366996559 hasRelatedWork W2562935373 @default.
- W4366996559 hasRelatedWork W2789027598 @default.
- W4366996559 hasRelatedWork W2803578821 @default.
- W4366996559 hasRelatedWork W2904944300 @default.
- W4366996559 hasRelatedWork W4366996559 @default.
- W4366996559 hasRelatedWork W95929880 @default.
- W4366996559 hasVolume "31" @default.
- W4366996559 isParatext "false" @default.
- W4366996559 isRetracted "false" @default.
- W4366996559 workType "article" @default.