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• W4366996731 abstract "Isolated individual myofibers are valuable experimental models that can be used in various conditions to understand skeletal muscle physiology and pathophysiology at the tissue and cellular level. This report details a time- and cost-effective method for isolation of single myofibers from the flexor digitorum brevis (FDB) muscle in both young and aged mice. The FDB muscle was chosen for its documented history in single myofiber experiments. By modifying published methods for FDB myofiber isolation, we have optimized the protocol by first separating FDB muscle into individual bundles before the digestion, followed by optimizing the subsequent digestion medium conditions to ensure reproducibility. Morphological and functional assessments demonstrate a high yield of isolated FDB myofibers with sarcolemma integrity achieved in a shorter time frame than previous published procedures. This method could be also adapted to other types of skeletal muscle. Additionally, this highly reproducible method can greatly reduce the number of animals needed to yield adequate numbers of myofibers for experiments. Thus, this advanced method for myofiber isolation has the potential to accelerate research in skeletal muscle physiology and screening potential therapeutics “ex vivo” for muscle diseases and regeneration. Single myofiber isolation can be a challenging step for researchers conducting muscle electrophysiology, live cell imaging studies, and so on. We have developed a modified method to produce consistently higher yield of healthy and functional isolated myofibers with a time and cost saving procedure. The implementation of this method could significantly increase the capacities of ex vivo studies in the skeletal muscle research field. (Color images are available online)." @default.
• W4366996731 created "2023-04-27" @default.
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• W4366996731 date "2023-08-01" @default.
• W4366996731 modified "2023-09-30" @default.
• W4366996731 title "Advanced Methodology for Rapid Isolation of Single Myofibers from Flexor Digitorum Brevis Muscle" @default.
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• W4366996731 doi "https://doi.org/10.1089/ten.tec.2023.0012" @default.
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