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• W4367042159 abstract "The composition of voltage-gated Ca 2+ channel (Ca v ) subtypes that gate action potential (AP)-evoked release changes during the development of mammalian CNS synapses. Ca v 2.2 and Ca v 2.3 lose their function in gating-evoked release during postnatal synapse maturation. In mature boutons, Ca v 2.1 currents provide the almost exclusive trigger for evoked release, and Ca v 2.3 currents are required for the induction of presynaptic long-term potentiation. However, the functional significance of Ca v 2.2 remained elusive in mature boutons, although they remain present at active zones and continue contributing significantly to presynaptic Ca 2+ influx. Here, we addressed the functional significance of Ca v 2.2 and Ca v 2.3 at mature parallel-fiber (PF) to Purkinje neuron synapses of mice of either sex. These synapses are known to exhibit the corresponding developmental Ca v subtype changes in gating release. We addressed two hypotheses, namely that Ca v 2.2 and Ca v 2.3 are involved in triggering spontaneous glutamate release and that they are engaged in vesicle recruitment during repetitive evoked release. We found that spontaneous miniature release is Ca 2+ dependent. However, experiments with Ca v subtype-specific blockers excluded the spontaneous opening of Ca v s as the Ca 2+ source for spontaneous glutamate release. Thus, neither Ca v 2.2 nor Ca v 2.3 controls spontaneous release from PF boutons. Furthermore, vesicle recruitment during brief bursts of APs was also independent of Ca 2+ influx through Ca v 2.2 and Ca v 2.3. However, Ca v 2.2, but not Ca v 2.3, currents significantly boosted vesicle recruitment during sustained high-frequency synaptic transmission. Thus, in mature PF boutons Ca v 2.2 channels are specifically required to sustain synaptic transmission during prolonged neuronal activity. SIGNIFICANCE STATEMENT At young CNS synapses, action potential-evoked release is gated via three subtypes of voltage-gated Ca 2+ channels: Ca v 2.1, Ca v 2.2, and Ca v 2.3. During postnatal maturation, Ca v 2.2 and Ca v 2.3 lose their function in gating evoked release, such that at mature synapses Ca v 2.1 provides the almost exclusive source for triggering evoked release. Ca v 2.3 currents are required for the induction of presynaptic long-term potentiation. However, the function of the still abundant Ca v 2.2 in mature boutons remained largely elusive. Here, we studied mature cerebellar parallel-fiber synapses and found that Ca v 2.2 does not control spontaneous release. However, Ca 2+ influx through Ca v 2.2 significantly boosted vesicle recruitment during trains of action potentials. Thus, Ca v 2.2 in mature parallel-fiber boutons participate in sustaining synaptic transmission during prolonged activity." @default.
• W4367042159 created "2023-04-27" @default.
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• W4367042159 date "2023-04-26" @default.
• W4367042159 modified "2023-10-14" @default.
• W4367042159 title "Ca_v2.2 Channels Sustain Vesicle Recruitment at a Mature Glutamatergic Synapse" @default.
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• W4367042159 cites W1966667764 @default.
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• W4367042159 cites W2008456025 @default.
• W4367042159 cites W2010046528 @default.
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• W4367042159 cites W2011057572 @default.
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• W4367042159 cites W2072747721 @default.
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• W4367042159 doi "https://doi.org/10.1523/jneurosci.1279-22.2023" @default.
• W4367042159 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37185239" @default.
• W4367042159 hasPublicationYear "2023" @default.
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