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- W4367047650 abstract "Strategies for neuroprotection are the main targets of glaucoma research. The neuroprotective properties of SRT2104 administration have been proven in central nervous system degeneration diseases through the activation of nicotinamide adenine dinucleotide-dependent deacetylase-silence information regulator 1 (Sirt1). Here, we investigated whether SRT2104 could protect the retina from ischemia/reperfusion (I/R) injury and the underlying mechanisms.SRT2104 was intravitreally injected immediately after I/R induction. RNA and protein expression were detected by quantitative real-time PCR and Western blot. Protein expression and distribution were examined by immunofluorescence staining. Retinal structure and function were analyzed by hematoxylin and eosin staining, optical coherence tomography, and electroretinogram. Optic nerve axons were quantified using toluidine blue staining. Cellular apoptosis and senescence were evaluated by TUNEL assay and SA-β-gal staining.The protein expression of Sirt1 decreased dramatically after I/R injury and SRT2104 administration effectively enhanced the stability of Sirt1 protein without significantly influencing Sirt1 mRNA synthesis. SRT2104 administration alone exerted no influence on the structure and function of normal retinas. However, SRT2104 intervention significantly protected the inner retinal structure and neurons; partially restored retinal function after I/R injury. I/R-induced cellular apoptosis and senescence were effectively alleviated by SRT2104 administration. Additionally, SRT2104 intervention markedly reduced neuroinflammation, including reactive gliosis, retinal vascular inflammation, and the overexpression of pro-inflammatory cytokines after I/R injury. Mechanistically, I/R-induced acetylation of p53, NF-κB p65, and STAT3 was significantly reversed by SRT2104 intervention.We demonstrated that SRT2104 exerted potent protective effects against I/R injury by enhancing Sirt1-mediated deacetylation and suppressing apoptosis, senescence, and neuroinflammation-related pathways." @default.
- W4367047650 created "2023-04-27" @default.
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- W4367047650 date "2023-04-26" @default.
- W4367047650 modified "2023-10-04" @default.
- W4367047650 title "Neuroprotection of SRT2104 in Murine Ischemia/Reperfusion Injury Through the Enhancement of Sirt1-Mediated Deacetylation" @default.
- W4367047650 cites W1736016875 @default.
- W4367047650 cites W1843851062 @default.
- W4367047650 cites W1938436444 @default.
- W4367047650 cites W1970688878 @default.
- W4367047650 cites W1973035180 @default.
- W4367047650 cites W1979504051 @default.
- W4367047650 cites W1996609490 @default.
- W4367047650 cites W2009668465 @default.
- W4367047650 cites W2034742711 @default.
- W4367047650 cites W2036229348 @default.
- W4367047650 cites W2038666430 @default.
- W4367047650 cites W2058407555 @default.
- W4367047650 cites W2070831723 @default.
- W4367047650 cites W2084726394 @default.
- W4367047650 cites W2086641231 @default.
- W4367047650 cites W2093055679 @default.
- W4367047650 cites W2095145874 @default.
- W4367047650 cites W2097126488 @default.
- W4367047650 cites W2115554047 @default.
- W4367047650 cites W2122563776 @default.
- W4367047650 cites W2135387645 @default.
- W4367047650 cites W2141327052 @default.
- W4367047650 cites W2146819974 @default.
- W4367047650 cites W2164738822 @default.
- W4367047650 cites W2165368743 @default.
- W4367047650 cites W2166529463 @default.
- W4367047650 cites W2170932556 @default.
- W4367047650 cites W226663039 @default.
- W4367047650 cites W2288150943 @default.
- W4367047650 cites W2319041743 @default.
- W4367047650 cites W2323333461 @default.
- W4367047650 cites W2387443869 @default.
- W4367047650 cites W2471832018 @default.
- W4367047650 cites W2515158101 @default.
- W4367047650 cites W2536646728 @default.
- W4367047650 cites W2560232547 @default.
- W4367047650 cites W2566969499 @default.
- W4367047650 cites W2610789056 @default.
- W4367047650 cites W2702840193 @default.
- W4367047650 cites W2730055111 @default.
- W4367047650 cites W2761604622 @default.
- W4367047650 cites W2800360805 @default.
- W4367047650 cites W2807277851 @default.
- W4367047650 cites W2886053196 @default.
- W4367047650 cites W2894569443 @default.
- W4367047650 cites W2900782291 @default.
- W4367047650 cites W2910084553 @default.
- W4367047650 cites W2913193276 @default.
- W4367047650 cites W2921835848 @default.
- W4367047650 cites W2944342612 @default.
- W4367047650 cites W2946801120 @default.
- W4367047650 cites W2957659820 @default.
- W4367047650 cites W2966646702 @default.
- W4367047650 cites W2979917512 @default.
- W4367047650 cites W2990476750 @default.
- W4367047650 cites W2999529756 @default.
- W4367047650 cites W3010215457 @default.
- W4367047650 cites W3011798321 @default.
- W4367047650 cites W3027536595 @default.
- W4367047650 cites W3047294486 @default.
- W4367047650 cites W3049029268 @default.
- W4367047650 cites W3088453717 @default.
- W4367047650 cites W3090224263 @default.
- W4367047650 cites W3090668231 @default.
- W4367047650 cites W3091877836 @default.
- W4367047650 cites W3093260385 @default.
- W4367047650 cites W3094587651 @default.
- W4367047650 cites W3104721145 @default.
- W4367047650 cites W3121830935 @default.
- W4367047650 cites W3136762451 @default.
- W4367047650 cites W3165780670 @default.
- W4367047650 cites W3187045755 @default.
- W4367047650 cites W3209655457 @default.
- W4367047650 cites W4207021552 @default.
- W4367047650 cites W4221072662 @default.
- W4367047650 cites W4224081637 @default.
- W4367047650 cites W4229489493 @default.
- W4367047650 cites W4229554506 @default.
- W4367047650 cites W4230825363 @default.