Matches in SemOpenAlex for { <https://semopenalex.org/work/W4367050147> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W4367050147 abstract "Abstract Background: Mastitis is a major cause of economic loss for dairy farmers and industry. River buffalo play an economically significant role in Egypt. Buffaloes with mastitis have reduced milk yield and change in milk composition. Genetic variations in the TLR4 gene have been related to several diseases in farm animals and humans including mastitis. The present investigation aims to find the genotypic variations in the TLR4 gene and their relation to mastitis in the river buffalo, Egyptian breed. Results: Eighty-one buffaloes (Egyptian breed) were tested for mastitis using SCC. 45% of the investigated buffaloes had mastitis. DNA from 30 buffaloes' blood samples (15 healthy and 15 with mastitis) were extracted and the TLR4 gene was sequenced. Twenty-one SNPs were found from which four SNPs were associated with mastitis: one in 5'UTR (c.1-g27) and 3 SNPs in the coding region at c.87, c.575, and c.576. The nucleotide variations in SNPs c.1-g27(C>A) and c.87 (C>A) were only present in buffalo with mastitis, while buffaloes with genotype CC at both locations were healthy. The AA genotype at c.87 (P.29) results in a stop codon leading to an abnormally shortened protein. The nonsynonymous SNPs c.575 A>G, and c.576 T>G shared amino acid 192 resulting in three amino acids ( His 192 Arg/Gln ). The dominant genotypes AA at c.575 and TT at c.576 were associated with mastitis resistance (OR<1.00), while recessive genotype GG at c.575 was associated with mastitis susceptibility (OR> 1.00). These two SNPs may affect their role in ligand recognition since they were in the LRR4 domain (p.174-p.197) which is part of coreceptor binding region 1. Conclusion: The present study confirms the relation between TLR4 genotypes and mastitis resistance or mastitis susceptibility in river buffalo. The study suggested four SNPs c1-g27C>A, c.87 C>A, c.575A>G, c. 576T>G,) have the potential to be markers for assisted buffalo selection to improve milk production." @default.
- W4367050147 created "2023-04-27" @default.
- W4367050147 creator A5016587518 @default.
- W4367050147 creator A5050396597 @default.
- W4367050147 creator A5056964601 @default.
- W4367050147 creator A5070258753 @default.
- W4367050147 creator A5084452225 @default.
- W4367050147 date "2023-04-26" @default.
- W4367050147 modified "2023-09-30" @default.
- W4367050147 title "Sequence analysis of TLR4 gene in river buffalo (Egyptian breed) and SNPs association with Mastitis" @default.
- W4367050147 cites W1869362176 @default.
- W4367050147 cites W1963785530 @default.
- W4367050147 cites W1963827945 @default.
- W4367050147 cites W1986052203 @default.
- W4367050147 cites W1998142035 @default.
- W4367050147 cites W2010669735 @default.
- W4367050147 cites W2011968640 @default.
- W4367050147 cites W2036453390 @default.
- W4367050147 cites W2044905101 @default.
- W4367050147 cites W2062835892 @default.
- W4367050147 cites W2085024439 @default.
- W4367050147 cites W2090768116 @default.
- W4367050147 cites W2118094114 @default.
- W4367050147 cites W2119202998 @default.
- W4367050147 cites W2123577868 @default.
- W4367050147 cites W2124831299 @default.
- W4367050147 cites W2158589826 @default.
- W4367050147 cites W2210888365 @default.
- W4367050147 cites W2253334047 @default.
- W4367050147 cites W2280303199 @default.
- W4367050147 cites W2621730475 @default.
- W4367050147 cites W2772326780 @default.
- W4367050147 cites W3038009201 @default.
- W4367050147 cites W3092750669 @default.
- W4367050147 cites W3097809385 @default.
- W4367050147 cites W3927593 @default.
- W4367050147 doi "https://doi.org/10.21203/rs.3.rs-2780630/v1" @default.
- W4367050147 hasPublicationYear "2023" @default.
- W4367050147 type Work @default.
- W4367050147 citedByCount "0" @default.
- W4367050147 crossrefType "posted-content" @default.
- W4367050147 hasAuthorship W4367050147A5016587518 @default.
- W4367050147 hasAuthorship W4367050147A5050396597 @default.
- W4367050147 hasAuthorship W4367050147A5056964601 @default.
- W4367050147 hasAuthorship W4367050147A5070258753 @default.
- W4367050147 hasAuthorship W4367050147A5084452225 @default.
- W4367050147 hasBestOaLocation W43670501471 @default.
- W4367050147 hasConcept C104317684 @default.
- W4367050147 hasConcept C135763542 @default.
- W4367050147 hasConcept C140793950 @default.
- W4367050147 hasConcept C153209595 @default.
- W4367050147 hasConcept C2776344049 @default.
- W4367050147 hasConcept C2776482104 @default.
- W4367050147 hasConcept C42972112 @default.
- W4367050147 hasConcept C54355233 @default.
- W4367050147 hasConcept C71924100 @default.
- W4367050147 hasConcept C86803240 @default.
- W4367050147 hasConcept C89423630 @default.
- W4367050147 hasConceptScore W4367050147C104317684 @default.
- W4367050147 hasConceptScore W4367050147C135763542 @default.
- W4367050147 hasConceptScore W4367050147C140793950 @default.
- W4367050147 hasConceptScore W4367050147C153209595 @default.
- W4367050147 hasConceptScore W4367050147C2776344049 @default.
- W4367050147 hasConceptScore W4367050147C2776482104 @default.
- W4367050147 hasConceptScore W4367050147C42972112 @default.
- W4367050147 hasConceptScore W4367050147C54355233 @default.
- W4367050147 hasConceptScore W4367050147C71924100 @default.
- W4367050147 hasConceptScore W4367050147C86803240 @default.
- W4367050147 hasConceptScore W4367050147C89423630 @default.
- W4367050147 hasLocation W43670501471 @default.
- W4367050147 hasOpenAccess W4367050147 @default.
- W4367050147 hasPrimaryLocation W43670501471 @default.
- W4367050147 hasRelatedWork W1988426099 @default.
- W4367050147 hasRelatedWork W2028661089 @default.
- W4367050147 hasRelatedWork W2049974637 @default.
- W4367050147 hasRelatedWork W2091392712 @default.
- W4367050147 hasRelatedWork W2387630014 @default.
- W4367050147 hasRelatedWork W2413494476 @default.
- W4367050147 hasRelatedWork W2755277876 @default.
- W4367050147 hasRelatedWork W3088500223 @default.
- W4367050147 hasRelatedWork W4313494259 @default.
- W4367050147 hasRelatedWork W4376891785 @default.
- W4367050147 isParatext "false" @default.
- W4367050147 isRetracted "false" @default.
- W4367050147 workType "article" @default.